Genetic differentiation of Abies equi-trojani (Asch. & Sint. ex Boiss) Mattf. populations from Kazdaǧι, Turkey and the genetic relationship between Turkish firs belonging to the Abies nordmanniana Spach complex

Kaya, Zeki
Neale, David Brian
The present study aimed to test the utility of RAPD (randomly amplified polymorphic DNA) and cpSSR (simple sequence repeats) markers for in situ gene conservation programs for fir species, as well as for determining the genetic similarities between the Abies nordmanniana Spach species complex (A. nordmanniana, A. bornmuelleriana Matff., A. equi-trojani (Asch. & Sint. ex Boiss) Mattf.) and between populations of A. equi-trojani, which is a narrow-endemic to Turkey. For this purpose, DNA was extracted and pooled from 15 seed megagametophytes (megs) of the Ortaköy population of A. nordmanniana and the Muratdere population of A. bornmuelleriana species, and from two 7-meg subsamples each from of the Kazdağı and Çan populations of A. equi-trojani. Template DNA was screened with the DNA markers to reveal the amount of genetic variation in each species. It appeared that template DNA pooling for screening the fir populations with RAPD or cp-SSR markers could be effectively used to speed up gene conservation and taxonomic studies. It is suggested that DNA pooling for template DNA in the PCR (polymerase chain reaction) mixture should be limited to 7 megs, but at least 5 replications that sample different sets of families each time. Based on genetic similarity and distance values, it seems that the Turkish fir species studied, as well as A. equi-trojani populations, have genetically been well differentiated. Fragmented distribution of 3 fir species in northern Turkey, belonging to the nordmanniana fir complex, also supports the possible existence of an ancestral species. However, to test this further studies that include fir species from the region, such as A. cephalonica Loud., A. borisii-regis Mattf., and A. cilicica Ant. & Kotschy, are needed.
Turkish Journal of Botany


Genetic structure of black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled from the Bolkar Mountains
Tolun, AA; Velioğlu, E; Çengel, Burcu N; Kaya, Zeki (2000-01-01)
In order to determine the magnitude and pattern of genetic diversity among Anatolian Black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled in Bolkar Mountains and to recommend the potential populations which may be suitable for in situ conservation of genetic resources in this species, isoenzymes from 14 enzyme systems were investigated by starch gel electrophoresis. For this reason, open pollinated seed megagametophytes of half-sib families originated from the four populations (Camliyay...
Genetic control of rooting ability of lodgepole pine cuttings
Fries, A; Kaya, Zeki (1997-11-01)
This study evaluates the genetic control of rooting ability ex vitro for cuttings of lodgepole pine (Pinus contorta var. latifolia Engelm.). More than 200 clones from 21 full-sib families were studied during 4 yr in the greenhouse. Clonal averages in rooting frequency, root size score, shoot extension during rooting, and vitality score were calculated, together with variance components and phenotypic and genetic correlations among variables. Years, families within latitude of origin, clones within families ...
Yilmaz, Remziye; CABİ, EVREN; Doğan, Musa (2014-06-01)
RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase Chain Reaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some species in the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used in the study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments which have been used as a measure of the degree of relationship b...
Genotyping of Anatolian doubled-haploid durum lines with SSR markers
Hakki, EE; Savaskan, C; Akkaya, Mahinur (2001-01-01)
In this study, doubled haploid lines generated from the durum wheat varieties, selections from Middle Anatolian landraces, 'Cakmak-79', 'Berkmen-469' and 'Kunduru-1149' (Savaskan et al., 1997), were analyzed using ten highly polymorphic microsatellite markers for genotyping and evaluation of genetic relationships between and within the doubled-haploid (DH) lines. The average PIC value was found to be 0.531. Populations of doubled-haploid lines of landrace selected cultivars 'Cakmak-79' and 'Kunduru-1149', w...
Ivanova, Evgeniya N.; Petrov, Plamen; Bouga, Maria; Emmanouel, Nicholas G.; TUNCA, RAHŞAN İVGİN; Kence, Meral (2010-01-01)
Genetic variation of honey bee populations from 9 different locations of Bulgaria was studied using 4 enzymic systems (MDH, ME, EST and ALP) corresponding to 4 genetic loci and PCR-RFLP's analysis of 16s rDNA, COI, and ND5 gene segments of mtDNA. Allozyme analysis revealed that all loci were polymorphic in almost all studied populations. The observed heterozygosity was found to range from 0.146 to 0.258, and Nei's genetic distance between 0.006 and 0.057 among the populations. Bulgarian honey bees are clust...
Citation Formats
Z. Kaya and D. B. Neale, “Genetic differentiation of Abies equi-trojani (Asch. & Sint. ex Boiss) Mattf. populations from Kazdaǧι, Turkey and the genetic relationship between Turkish firs belonging to the Abies nordmanniana Spach complex,” Turkish Journal of Botany, pp. 1–10, 2008, Accessed: 00, 2021. [Online]. Available: