NANOSIZED ZEOLITES AS A PERSPECTIVE MATERIAL FOR DEVELOPMENT OF ELECTROCHEMICAL ENZYME BIOSENSORS

Date

2015-09-15

Author

Kucherenko, Ivan
Soldatkin, Oleksandr
Saiapina, Olga
Soy, Esin
Ozansoy, Berna
Kirdeciler, Salih Kaan
Öztürk, Seçkin
Akata Kurç, Burcu
Dzyadevych, Sergei

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In this work, the method of enzyme adsorption on different zeolites and mesoporous silica spheres (MSS) was investigated for the creation of conductometric biosensors. The conductometric transducers consisted of gold interdigitated electrodes were placed on the ceramic support. The transducers were modified with zeolites and MSS, and then the enzymes were adsorbed on the transducer surface. Different methods of zeolite attachment to the transducer surface were used; drop coating with heating to 200°C turned out to be the best one. Nanozeolites beta and L, zeolite L, MSS, and silicalite-1 (80 to 450 nm) were tested as the adsorbents for enzyme urease. The biosensors with all tested particles except zeolite L had good analytical characteristics. Silicalite-1 (450 nm) was also used for adsorption of glucose oxidase, acetylcholinesterase, and butyrylcholinesterase. The glucose and acetylcholine biosensors were successfully created, whereas butyrylcholinesterase was not adsorbed on silicalite-1. The enzyme adsorption on zeolites and MSS is simple, quick, well reproducible, does not require use of toxic compounds, and therefore can be recommended for the development of biosensors when these advantages are especially important.

Subject Keywords

Biosensor, Conductometric transducer, Acetylcholinesterase, Urease, Enzyme adsorption, Mesoporous silica spheres, Zeolite, Silicalite-1

URI

https://hdl.handle.net/11511/79823
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429111/

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Unverified, Conference / Seminar

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Citation Formats

I. Kucherenko et al., “NANOSIZED ZEOLITES AS A PERSPECTIVE MATERIAL FOR DEVELOPMENT OF ELECTROCHEMICAL ENZYME BIOSENSORS,” 2015, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/79823.