Site Specific Mutations of Highly Conserved Residues in APH 3 II Phenotypic and Structural Analysis of Mutant Enzymes

Date

1992-05-26

Author

Kocabıyık, Semra

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https://hdl.handle.net/11511/80477

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Site - Specific Mutations of Conserved C - Terminal Residues in Aminoglycoside 3'-Phosphotransferase II [APH(3')-II]: Phenotypic and Structural Analysis of the Mutant Enzymes Kocabıyık, Semra (1992-06-01) In order to test the biological importance of amino acids in the C-terminal quarter of aminoglycoside 3′-phosphotransferase II enzyme, seven of the highly conserved residues in this region, His-188, Asp-190, Asp-208, Gly-210, Arg-211, Asp-216 and Asp-220, were changed via site-directed mutagenesis. The phenotype of each mutant was compared to wildtype in terms of antibiotic susceptibilities and enzymatic activities. All of the substitutions either abolished or significantly reduced the resistance of the res...
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Sequence specific inhibition of human chemokine gene, cxcl7, by small interfering RNAS in lewis lung carcinoma cells Kızışar, Dilay; Akkaya, Mahinur S.; Department of Chemistry (2013) In this study, we aimed to design small interfering RNAs to silence the expression of a gene that is a member of chemokines playing crucial roles on cancer. Small interfering RNAs (siRNAs) are generated by ribonuclease III cleavage of 20-25nt in length dsRNAs which mediate RNAi-induced specific mRNA degradation and they are known to be an important part of the gene regulation mechanism in all Eukaryotes. Recently, siRNAs are being utilized as promising treatment approaches. In this study, we targeted a gene...
Stepwise Reversal of Immune Dysregulation Due to STAT1 Gain-of-Function Mutation Following Ruxolitinib Bridge Therapy and Transplantation Kayaoğlu, Başak; Yilmaz, Naz Surucu; Charbonnier, Louis Marie; Geckin, Busranur; Akcay, Arzu; Eltan, Sevgi Bilgic; Ozturk, Gulyuz; ÖZEN, AHMET OĞUZHAN; Karakoc-Aydiner, Elif; Chatila, Talal A.; Gürsel, Mayda (2021-01-01) Purpose Patients with heterozygous gain-of-function (GOF) mutations in STAT1 frequently exhibit chronic mucocutaneous candidiasis (CMC), immunodeficiency and autoimmune manifestations. Several treatment options including targeted therapies and hematopoietic stem cell transplantation (HSCT) are available for STAT1 GOF patients but modalities and outcomes are not well established. Herein, we aimed to unravel the effect of ruxolitinib as a bridge therapy in a patient with sporadic STAT1 T385M mutation to manag...

Citation Formats

S. Kocabıyık, “Site Specific Mutations of Highly Conserved Residues in APH 3 II Phenotypic and Structural Analysis of Mutant Enzymes,” 1992, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/80477.