Changes in gene profile of human hepatocellular carcinoma cell line (HepG2) induced by boric acid at half mazimal inhibitory concentration (IC50).

2017-06-30
Tombuloğlu, Ayşegül
Çöpoğlu, Hülya
Güray, Nülüfer Tülün
Aydın Son, Yeşim
Show full item record
228
views
77
downloads
Boron is an essential micronutrient for many biological processes in plants and animals. In humans, adverse health outcomes are reported for both boron deprivation and excessive boron intake. Humans might get exposed to high doses of boron by consuming boron containing products or by working at boron enriched workplaces. In this study, the aim is to find out about the cytotoxicity mechanisms of boric acid, the most abundant form of boron containing compounds in human blood, on human hepatocellular carcinoma (HepG2) cell line. XTT cell proliferation assay was carried out to assess the effect of boric acid treatment at varying concentrations on the viability of HepG2 cells and to determine the half maximal inhibitory concentration (IC50) of boric acid. Comet assay and microarray experiments were performed at IC50 concentration to examine boric acid induced DNA damage and changes in gene expression profile. Boric acid inhibited cell growth in a dose dependent manner with an IC50 value of 24 mM. Microarray analysis revealed a total of 785 gene as differentially regulated in response to boric acid treatment. Analyses of differentially expressed transcripts for enriched gene ontologies and pathways using Database for Annotation, Visualization and Integrated Discovery (DAVID) Functional Annotation Tool suggest cell cycle arrest at mitotic phase and controlled cell death as potential mechanisms of inhibited cell growth. Our overall findings suggest, at IC50 concentration boric acid might be inhibiting DNA replication and possibly interfering with lipid and amino acid metabolism.
http://hibit2017.ii.metu.edu.tr/wordpress/wp-content/uploads/HIBIT2017_Conference_Book.pdf
https://hdl.handle.net/11511/81521
HIBIT 2017, 28 - 30 Haziran 2017
Department of Biology, Conference / Seminar

Suggestions

Changes in the chemical composition of an acidic soil treated with marble quarry and marble cutting wastes
TOZSİN, Gülşen; ÖZTAŞ, Taşkın; Arol, Ali İhsan; KALKAN, Ekrem (Elsevier BV, 2015-11-01)
Soil acidity greatly affects the availability of plant nutrients. The level of soil acidity can be adjusted by treating the soil with certain additives. The objective of this study was to determine the effect of marble quarry waste (MQW) and marble cutting waste (MCW) on the chemical composition and the acidity of a soil. Marble wastes at different rates were applied to an acid soil. Their effectiveness in neutralizing the soil pH was compared with that of agricultural lime. The changes in the chemical comp...
Monitoring of Tryptophan as a Biomarker for Cancerous Cells in Terahertz (THz) Sensing
Altan, Hakan; Gok, Seher; Ozyurt, Ipek; Severcan, Feride (2016-02-17)
Tryptophan is an extremely important amino acid for a variety of biological functions in living organisms. Changes in the concentration of this amino acid can point to identification of cancerous tissues or even confirm symptoms of depression in patients. Therefore it is extremely important to identify and quantify tryptophan concentrations in human blood as well as in in-vivo diagnostic studies. Here a reflection based terahertz pulsed spectroscopy system was used to study the interaction of THz pulses wit...
Extension of flower longevity in transgenic plants via antisense blockage of ethylene bıosynthesis
Deçani Yol, Betul; Öktem, Hüseyin Avni; Department of Biotechnology (2004)
Ethylene (C2H4) is a very simple molecule, a gas, and has numerous effects on the growth, development and storage life of many fruits, vegetables and ornamental crops. In higher plants, ethylene is produced from L-methionine in essentially all tissues and ACC Synthase and ACC Oxidase are the two key enzymes in the biosynthesis of ethylene. The objective of the present study was to transform tobacco (Nicotiana tabacum L. cv. Samsun) plant with partial sequence of torenia acc oxidase gene in antisense and sen...
Distinct patterns of sedimentary phosphorus fractionation and mobilization in the seafloor of the Black Sea, Marmara Sea and Mediterranean Sea
Akçay, İsmail; Yücel, Mustafa (2023-03-10)
Phosphorus (P) is a key element to all life that is used for structural and functional component of all organisms. The cycling of sedimentary P may differ depending on the redox-conditions of the overlying waters affecting the dynamics, and distribution of P-fractions and the elements that are highly coupled to P cycle. Though biogeochemistry of water column in the three interconnected marine basins of Black, Marmara and Mediterranean Seas have been studied extensively, few studies were carried out to under...
Changes of Eutrofication Indicator Parameters (TP, DIN, Chl-a and TRIX) in the Cilician Basin (Northeast Mediterranean)
TUĞRUL, SÜLEYMAN; Uysal, Zahit; Erdogan, Emine; Yucel, Nebil (2011-01-01)
Eutrophication processes and changes in coastal ecosystem are determined by the coastal/open sea interactions and rates of nutrient pollution. The Cilician Basin shelf waters are affected not only by intense industrial activities and increased population, but also by complex atmosphere-sea-land interactions. There is a recognizable difference between water qualities of Mersin bay coastal and the Cilician open waters depleted in nutrients. Eutrophic conditions have developed in the eastern bay shallow waters...
Citation Formats
A. Tombuloğlu, H. Çöpoğlu, N. T. Güray, and Y. Aydın Son, “Changes in gene profile of human hepatocellular carcinoma cell line (HepG2) induced by boric acid at half mazimal inhibitory concentration (IC50).,” presented at the HIBIT 2017, 28 - 30 Haziran 2017, Güzelyurt, Turkish Republic of Northern Cyprus (KKTC), 2017, Accessed: 00, 2021. [Online]. Available: http://hibit2017.ii.metu.edu.tr/wordpress/wp-content/uploads/HIBIT2017_Conference_Book.pdf.
METU IIS - Integrated Information Service open@metu.edu.tr