Changes in gene profile of human hepatocellular carcinoma cell line (HepG2) induced by boric acid at half mazimal inhibitory concentration (IC50).

2017-06-30
Tombuloğlu, Ayşegül
Çöpoğlu, Hülya
Güray, Nülüfer Tülün
Aydın Son, Yeşim
Boron is an essential micronutrient for many biological processes in plants and animals. In humans, adverse health outcomes are reported for both boron deprivation and excessive boron intake. Humans might get exposed to high doses of boron by consuming boron containing products or by working at boron enriched workplaces. In this study, the aim is to find out about the cytotoxicity mechanisms of boric acid, the most abundant form of boron containing compounds in human blood, on human hepatocellular carcinoma (HepG2) cell line. XTT cell proliferation assay was carried out to assess the effect of boric acid treatment at varying concentrations on the viability of HepG2 cells and to determine the half maximal inhibitory concentration (IC50) of boric acid. Comet assay and microarray experiments were performed at IC50 concentration to examine boric acid induced DNA damage and changes in gene expression profile. Boric acid inhibited cell growth in a dose dependent manner with an IC50 value of 24 mM. Microarray analysis revealed a total of 785 gene as differentially regulated in response to boric acid treatment. Analyses of differentially expressed transcripts for enriched gene ontologies and pathways using Database for Annotation, Visualization and Integrated Discovery (DAVID) Functional Annotation Tool suggest cell cycle arrest at mitotic phase and controlled cell death as potential mechanisms of inhibited cell growth. Our overall findings suggest, at IC50 concentration boric acid might be inhibiting DNA replication and possibly interfering with lipid and amino acid metabolism.
Citation Formats
A. Tombuloğlu, H. Çöpoğlu, N. T. Güray, and Y. Aydın Son, “Changes in gene profile of human hepatocellular carcinoma cell line (HepG2) induced by boric acid at half mazimal inhibitory concentration (IC50).,” presented at the HIBIT 2017, 28 - 30 Haziran 2017, Güzelyurt, Turkish Republic of Northern Cyprus (KKTC), 2017, Accessed: 00, 2021. [Online]. Available: http://hibit2017.ii.metu.edu.tr/wordpress/wp-content/uploads/HIBIT2017_Conference_Book.pdf.