Deposition of a Sorbent into a Recession on a Solid Support Provides a New, MechanicallyRobust Solid Phase Micro-Extraction Device

Date

2017-03-05

Author

Poole, Justen J
Bojko, Barbara
Boyacı, Ezel
Gómez-ríos, German A
Vander Heide, Harmen
Pawliszyn, Janusz
Grandy, Jonathan J
Reyes-garcés, Nathaly

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Traditional solid-phase microextraction fibers have been limited in their ease of use during in-vivo samplings due to the requirement for a sheathing needle to pre-puncture sample matrices. To address this limitation, a new SPME device is herein proposed which incorporates a coated region which is recessed into a solid support. As the coating surface is flush with, or lies below that of the solid support, the SPME coating does not directly experience the forces associated with the puncture of a sample matrix. The implementation of a recessed extractive coating has been verified as mechanically robust after being incorporated into a projectile which was fired from an air gun into whole fish resulting in no mechanical damage to the coating. In addition, the act of puncturing pre-slit septa in order to access a sample solution was verified to not affect extraction of analyte in a targeted study of pharmaceuticals in water at 15ppb. Next, the recessed SPME device was used for an in-vivo application where raw whole salmon steaks were directly sampled by pushing the recessed SPME device through the protective outer skin in order to sample the underlying muscle tissue for poly-unsaturated fatty acids. Method responses for these compounds were 3-4 times greater than commercially available mixed mode SPME fibers when a hydrophilic-lipophilic balanced coating was used. Certainly, the implementation of the presented device which requires no sheathing needle while being able to directly puncture tissue will provide minimally invasive in-vivo sampling opportunities for a wide range of applications.

Subject Keywords

Bioanalytical, Liquid Chromatography/Mass Spectroscopy, Sampling, SPME

URI

https://pittcon.org/2017/Pittcon-2017-Technical-Program.pdf

Conference Name

Pittcon 2017 (05 Mart 2017)

Collections

Department of Chemistry, Conference / Seminar

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Deposition of a Sorbent into a Recession on a Solid Support To Provide a New, Mechanically Robust Solid-Phase Microextraction Device Poole, Justen J.; Grandy, Jonathan J.; Yu, Miao; Boyacı, Ezel; Gomez-Rios, German A.; Reyes-Garces, Nathaly; Bojko, Barbara; Heide, Harmen Vander; Pawliszyn, Janusz (American Chemical Society (ACS), 2017-08-01) To date, solid-phase microextraction (SPME) fibers used for in vivo bioanalysis can be too fragile and flexible, which limits suitability for direct tissue sampling. As a result, these devices often require a sheathing needle to prepuncture robust sample matrixes and protect the extraction phase from mechanical damage. To address this limitation, a new SPME device is herein presented which incorporates an extraction phase recessed into the body of a solid needle. This device requires no additional support o...
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Citation Formats

J. J. Poole et al., “Deposition of a Sorbent into a Recession on a Solid Support Provides a New, MechanicallyRobust Solid Phase Micro-Extraction Device,” presented at the Pittcon 2017 (05 Mart 2017), Chicago, Illinois, USA, 2017, Accessed: 00, 2021. [Online]. Available: https://pittcon.org/2017/Pittcon-2017-Technical-Program.pdf.