Deposition of a Sorbent into a Recession on a Solid Support To Provide a New, Mechanically Robust Solid-Phase Microextraction Device

Date

2017-08-01

Author

Poole, Justen J.
Grandy, Jonathan J.
Yu, Miao
Boyacı, Ezel
Gomez-Rios, German A.
Reyes-Garces, Nathaly
Bojko, Barbara
Heide, Harmen Vander
Pawliszyn, Janusz

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

163
views

0
downloads

To date, solid-phase microextraction (SPME) fibers used for in vivo bioanalysis can be too fragile and flexible, which limits suitability for direct tissue sampling. As a result, these devices often require a sheathing needle to prepuncture robust sample matrixes and protect the extraction phase from mechanical damage. To address this limitation, a new SPME device is herein presented which incorporates an extraction phase recessed into the body of a solid needle. This device requires no additional support or shielding during puncture events through protective tissue. The presented device was, thoroughly tested, being fired at 90 m.s(-1) through fish scales, forced through vial septa, and employed in a targeted study of polyunsaturated fatty acids in salmon where the protective outer skin was repetitively punctured during sampling. Finally, the recessed SPME device was applied to an on-site application for the tissue analysis of wild muskellunge. With this advancement, rapid, minimally invasive, and easily executed in vivo SPME is now possible opening the door to near endless sampling opportunities.

Subject Keywords

Analytical Chemistry

URI

https://hdl.handle.net/11511/43851

Journal

ANALYTICAL CHEMISTRY

DOI

https://doi.org/10.1021/acs.analchem.7b01382

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Separation and quantitation of some platinum group metals by rp-hplc Alshana, Usama; Aygün, Rüveyde Sezer; Department of Chemistry (2004) In this study, a reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed to separate and determine Pt and Pd after formation of their chelates with N,N-diethyl-N'-benzoylthiourea (DEBT). With the aim of reducing the number of steps in treating the samples, the method developed does not require the elimination of excess chelating reagent before the analysis of metal chelates. The different physical and chemical parameters affecting separation were examined in details. The wh...
Immobilization studies utilizing solid supports for the determination of fructose by dansylaminophenyl boronic acid (DAPB acid) and chromate by diphenylcarbazide (DPC) Bulut, Mukadder; Volkan, Mürvet; Department of Chemistry (2006) Immobilization of fluorescent chemosensors and chromogenic reagents on solid supports for developing optical sensors result in improved analytical performance characteristics such as continuous read-out, increased sensitivity, lower reagent consumption and possibility of using the sensor in solvents where the free molecule displays low solubility. The aim of this study is to immobilize dansylaminophenyl boronic acid (DAPB acid) and diphenylcarbazide (DPC) into various solid supports for the determination of...
Determination of Arsenobetaine in Fish Tissue by Species Specific Isotope Dilution LC-LTQ-Orbitrap-MS and Standard Addition LC-ICPMS Yang, Lu; Ding, Jianfu; Maxwell, Paulette; McCooeye, Margaret; Windust, Anthony; Ouerdane, Laurent; Bakirdere, Sezgin; Willie, Scott; Mester, Zoltan (American Chemical Society (ACS), 2011-05-01) An accurate and precise method for the determination of arsenobetaine (AsB, (CH(3))(3)(+)AsCH(2)COO(-)) in fish samples using exact matching species specific isotope dilution (ID) liquid chromatography LTQ:Orbitrap mass spectrometry (LC-LTQ-Orbitrap-MS) and standard addition LC inductively coupled plasma mass spectrometry (LC-ICPMS) is described. Samples were extracted by sonication for 30 min with high purity deionized water. An in-house synthesized (13)C enriched AsB spike was used for species specific ID...
Preconcentration of volatile elements on quartz surface prior to determination by atomic spectrometry Korkmaz, Deniz; Ataman, Osman Yavuz; Department of Chemistry (2004) Hydride generation technique is frequently used for the detection of elements as As, Se, Sb, Sn, Bi, Ge, Te and Pb that form volatile hydrides in solution using a reductant. In this study, a novel quartz trap for on-line preconcentration of volatile analyte species was designed. Pb, Sb and Cd were selected as analyte elements and chemical vapour generation technique was employed for generation of their volatile species in flow systems. The trapping medium was formed by external heating of either the inlet a...
Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer Yildiz, Huseyin Bekir; Castillo, Jaime; Guschin, Dmitrii A.; Toppare, Levent Kamil; Schuhmann, Wolfgang (Springer Science and Business Media LLC, 2007-06-01) An amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most import...

Citation Formats

J. J. Poole et al., “Deposition of a Sorbent into a Recession on a Solid Support To Provide a New, Mechanically Robust Solid-Phase Microextraction Device,” ANALYTICAL CHEMISTRY, pp. 8021–8026, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43851.