Capture and release of viable CTCs in microfluidic channel

Date

2017-10-04

Author

Ateş, Hatice Ceren
Şen Doğan, Begüm
Özgür, Ebru
Külah, Haluk

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The number of circulating tumor cells (CTCs) in blood is associated with prognosis in several types of cancer. Isolation and characterization of CTCs have important clinical significance in terms of prognosis and early detection of response to treatment. Moreover, downstream characterization of CTCs may help better patient stratification and therapy guidance. However, CTCs are extremely rare and highly sensitive and specific technology is required to isolate viable CTCs from blood cells. In this study, a surface modification strategy is developed for (i) on-chip detection of CTCs by using immunostaining and (ii) viable cell release for downstream analysis. In the first application, selective CTC capture is demonstrated using breast cancer cells (MCF-7) spiked in buffer containing background leukocytes with cell concentration ratio of 1:100. Cells captured by anti-EpCAM coated microchannel are stained with immunofluorescence conjugated anti-pan cytokeratin antibody for epithelial cells and anti-CD45 antibody for hematologic cells, and by DAPI for nuclear staining. Cytokeratin and DAPI stained cells are scored as CTCs whereas CD45+ cells are scored as leukocytes. In the second application, captured cells are released with the combination of enzymatic treatment and high flow rate washing. The viability of released cells is confirmed by observing cell growth in culture.

URI

https://hdl.handle.net/11511/86857

Conference Name

9th Annual Lab-on-a-Chip and Microfluidics World Congress, 2017

Collections

Department of Electrical and Electronics Engineering, Conference / Seminar

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Citation Formats

H. C. Ateş, B. Şen Doğan, E. Özgür, and H. Külah, “Capture and release of viable CTCs in microfluidic channel,” presented at the 9th Annual Lab-on-a-Chip and Microfluidics World Congress, 2017, California, USA, 2017, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/86857.