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Development of Calcitriol Doxorubicin Co loaded Liposomes and İnvestigation of in vitro Cytotoxicity on Namalwa Cells
Date
2015-10-24
Author
Dalgıç, Ali Deniz
Tezcaner, Ayşen
Elçi, Pınar
Sarper, Meral
Arpacı, Fikret
Avcu, Ferit
Keskin, Dilek
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https://hdl.handle.net/11511/87834
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Dalgıç, Ali Deniz; Tezcaner, Ayşen; Elçi, Pınar; Sarper, Meral; Arpacı, Fikret; Avcu, Ferit; Keskin, Dilek (null; 2015-10-24)
Development of ACE2 loaded decoy liposomes and their effect on SARS-CoV-2 for Covid-19 treatment
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SARS-CoV-2 is a novel coronavirus with a positively oriented single-stranded RNA that first appeared in December 2019. In this study, Angiotensin Converting Enzyme 2 (ACE2) loaded decoy liposomes were developed and characterized. ACE2 protein was loaded onto a liposomal carrier system and its toxicity and effectiveness were evaluated in cell culture and in vitro virus neutralization studies. Liposomes were prepared with the film hydration method and adjusted for size with the dialysis membrane method or the...
Development of random amplified polymorphic DNA markers for genetic mapping in Pacific yew (Taxus brevifolia)
Gocmen, B; Jermstad, KD; Neale, DB; Kaya, Zeki (Canadian Science Publishing, 1996-03-01)
A genetic linkage map was constructed for Pacific yew (Taxus brevifolia Nutt.) based on random amplified polymorphic DNA (RAPD) markers. A series of optimization experiments were conducted to develop a highly repeatable protocol for Pacific yew. In these experiments, a high MgCl2 concentration (5.5 mM) together with a low primer concentration (0.2 mu m) in the polymerase chain reaction (PCR) mixture yielded the best amplification products. PCR amplification products were further improved by treating the tem...
Development and investigation of etoposide resistance in MCF-7 Breast cancer cell line
Kaplan, Esra; Gündüz, Ufuk; Department of Biology (2010)
Failure of chemotherapy in cancer patients because of development of drug resistance is a major problem. Alterations of DNA repair mechanisms and drug targets are among the important resistance mechanisms which are developed against topoisomerase II inhibitors etoposide and doxorubicin. Modifications in the expression levels of mismatch repair (MMR) genes due to resistance to topoisomerase II inhibitors are involved in breast cancer. In this study, etoposide resistant sublines were developed from MCF7 breas...
Development and validation of a resistance and virulence gene microarray targeting Escherichia coli and Salmonella enterica
Davis, Margaret A.; Lim, Ji Youn; Soyer, Yeşim; Harbottle, Heather; Chang, Yung-Fu; New, Daniel; Orfe, Lisa H.; Besser, Thomas E.; Call, Douglas R. (Elsevier BV, 2010-07-01)
A microarray was developed to simultaneously screen Escherichia coli and Salmonella enterica for multiple genetic traits. The final array included 203 60-mer oligonucleotide probes, including 117 for resistance genes, 16 for virulence genes, 25 for replicon markers, and 45 other markers. Validity of the array was tested by assessing inter-laboratory agreement among four collaborating groups using a blinded study design. Internal validation indicated that the assay was reliable (area under the receiver-opera...
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A. D. Dalgıç et al., “Development of Calcitriol Doxorubicin Co loaded Liposomes and İnvestigation of in vitro Cytotoxicity on Namalwa Cells,” 2015, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/87834.