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Hydrogen gas production by halobacterium halobium coupled to escherichia coli in a photobioreactor
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050992.pdf
Date
1995
Author
Kaya, Banu
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https://hdl.handle.net/11511/8872
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Graduate School of Natural and Applied Sciences, Thesis
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Rhodobacter sphaeroides O.U.001 is a photosynthetic non-sulfur bacterium which produces hydrogen from organic compounds under anaerobic conditions. Halobacterium salinarum is an archaeon and lives under extremely halophilic conditions (4 M NaCl). H. salinarum contains a retinal protein bacteriorhodopsin in its purple membrane which acts as a light-driven proton pump. In this study the Rhodobacter sphaeroides O.U.001 culture was combined with different amounts of packed cells of H. salinarum S9 or isolated p...
Hydrogen gas production using coupled enzyme systems from E-coli and Halobacterium halobium
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A photobioreactor containing enzyme systems from E.coli and Halobacterium halobium for hydrogen gas production was constructed. Bacteriorhodopsin is a retinal protein present in the purple membrane fragment of photosynthetic bacteria H. halobium which behaves as a light driven proton pump upon illumination. The H+ ion gradient produced between interior and exterior of the cell membrane utilized by the bacterial cell for synthesis of ATP as an energy source for its growth and survival. Under anaerobic growth...
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Rhodobacter sphaeroides O.U.001 can produce hydrogen under anaerobic conditions and illumination. The objective of this study was to investigate the performance of an 81 flat plate solar bioreactor operating in outdoor conditions. Different organic acids were used as carbon sources (malate, lactate and acetate) and olive mill waste water was used as a sole substrate source. The consumption and the production of the organic acids were determined by HPLC. The accumulation of by-products, such as poly-beta-hyd...
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B. Kaya, “Hydrogen gas production by halobacterium halobium coupled to escherichia coli in a photobioreactor,” Middle East Technical University, 1995.