Hydrogen gas production by combined systems of Rhodobacter sphaeroides OU001 and Halobacterium salinarum in a photobioreactor

Date

2006-09-01

Author

Zabut, Baker
EI-Kahlout, Kamal
Yucel, Meral
Gündüz, Ufuk
Turker, Lemi
Eroglu, Inci

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Rhodobacter sphaeroides O.U.001 is a photosynthetic non-sulfur bacterium which produces hydrogen from organic compounds under anaerobic conditions. Halobacterium salinarum is an archaeon and lives under extremely halophilic conditions (4 M NaCl). H. salinarum contains a retinal protein bacteriorhodopsin in its purple membrane which acts as a light-driven proton pump. In this study the Rhodobacter sphaeroides O.U.001 culture was combined with different amounts of packed cells of H. salinarum S9 or isolated purple membrane fragments in order to increase the photofermentative hydrogen gas production. The packed cells of H. salinarum have the ability to pump protons upon illumination due to the presence of bacteriorhodopsin. The proton gradient produced may be used for the formation of ATP or protons may be used for H-2 production by R. sphaeroides. Similar to intact cells purple membrane fragments may also form vesicles around certain ions and may act like closed systems.

Subject Keywords

Rhodobacter sphaeroides, Halobacterium salinarum, Biohydrogen, Bacteriorhodopsin, Photobioreactor, Combined systems

URI

https://hdl.handle.net/11511/31014

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2006.06.023

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

B. Zabut, K. EI-Kahlout, M. Yucel, U. Gündüz, L. Turker, and I. Eroglu, “Hydrogen gas production by combined systems of Rhodobacter sphaeroides OU001 and Halobacterium salinarum in a photobioreactor,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 1553–1562, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31014.