Hydrogen gas production by combined systems of Rhodobacter sphaeroides OU001 and Halobacterium salinarum in a photobioreactor

Zabut, Baker
EI-Kahlout, Kamal
Yucel, Meral
Gündüz, Ufuk
Turker, Lemi
Eroglu, Inci
Rhodobacter sphaeroides O.U.001 is a photosynthetic non-sulfur bacterium which produces hydrogen from organic compounds under anaerobic conditions. Halobacterium salinarum is an archaeon and lives under extremely halophilic conditions (4 M NaCl). H. salinarum contains a retinal protein bacteriorhodopsin in its purple membrane which acts as a light-driven proton pump. In this study the Rhodobacter sphaeroides O.U.001 culture was combined with different amounts of packed cells of H. salinarum S9 or isolated purple membrane fragments in order to increase the photofermentative hydrogen gas production. The packed cells of H. salinarum have the ability to pump protons upon illumination due to the presence of bacteriorhodopsin. The proton gradient produced may be used for the formation of ATP or protons may be used for H-2 production by R. sphaeroides. Similar to intact cells purple membrane fragments may also form vesicles around certain ions and may act like closed systems.


Hydrogen production by Rhodobacter sphaeroides OU001 in a flat plate solar bioreactor
Eroglu, Inci; Tabanoglu, Altan; Gündüz, Ufuk; Eroglu, Ela; Yucel, Meral (2008-01-01)
Rhodobacter sphaeroides O.U.001 can produce hydrogen under anaerobic conditions and illumination. The objective of this study was to investigate the performance of an 81 flat plate solar bioreactor operating in outdoor conditions. Different organic acids were used as carbon sources (malate, lactate and acetate) and olive mill waste water was used as a sole substrate source. The consumption and the production of the organic acids were determined by HPLC. The accumulation of by-products, such as poly-beta-hyd...
Kinetic analysis of photosynthetic growth, hydrogen production and dual substrate utilization by Rhodobacter capsulatus
Sevinc, Pelin; Gündüz, Ufuk; EROĞLU, İNCİ; Yucel, Meral (2012-11-01)
Rhodobacter capsulatus is purple non-sulfur (PNS) bacterium which can produce hydrogen and CO2 by utilizing volatile organic acids in presence of light under anaerobic conditions. Photofermentation by PNS bacteria is strongly affected by temperature and light intensity. In the present study we present the kinetic analysis of growth, hydrogen production, and dual consumption of acetic acid and lactic acid at different temperatures (20, 30 and 38 degrees C) and light intensities (1500, 2000, 3000, 4000 and 50...
Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides
Ipekoglu, Emre M.; Gocmen, Koray; Oz, Mehmet T.; Gurgan, Muazzez; Yucel, Meral (2017-03-01)
Rhodobacter sphaeroides is a purple non-sulfur bacterium which photoheterotrophically produces hydrogen from organic acids under anaerobic conditions. A gene coding for putative chlorophyll a synthase (chlG) from cyanobacterium Prochlorococcus marinus was amplified by nested polymerase chain reaction and cloned into an inducible-expression plasmid which was subsequently transferred to R. sphaeroides for heterologous expression. Induced expression of chlG in R. sphaeroides led to changes in light absorption ...
Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors
Uyar, Basar; EROĞLU, İNCİ; Yucel, Meral; Gündüz, Ufuk; Turker, Lemi (2007-12-01)
Rhodobacter sphaeroides O.U. 001 is a purple non-sulfur bacterium which evolves hydrogen from the breakdown of organic acids under illumination and anaerobic conditions. In this study, the effect of light intensity, light wavelength and illumination protocol on the growth and hydrogen production of R. sphaeroides O.U. 001 was investigated in gas-tight glass photobioreactors with defined medium. The results showed Also it was found that the rate of hydrogen production increased with increasing light intensit...
Hydrogen productivity of photosynthetic bacteria on dark fermenter effluent of potato steam peels hydrolysate
Afsar, Nilufer; Ozgur, Ebru; Gurgan, Muazzez; Akkose, Sevilay; Yucel, Meral; Gündüz, Ufuk; Eroglu, Inci (2011-01-01)
Hydrogen productivities of different photosynthetic bacteria have been searched on real thermophilic dark fermentation effluents (DFE). The results obtained with potato steam peels hydrolysate (PSP) DFE were compared to glucose DFE. Photobiological hydrogen production has been carried out in indoor, batch photobioreactors using several strains of purple non-sulfur (PNS) bacteria such as Rhodobacter capsulatus (DSM1710), Rhodobacter capsulatus hup- (YO3), Rhodobacter sphaeroides O.U.001 (DSM5864), Rb. sphaer...
Citation Formats
B. Zabut, K. EI-Kahlout, M. Yucel, U. Gündüz, L. Turker, and I. Eroglu, “Hydrogen gas production by combined systems of Rhodobacter sphaeroides OU001 and Halobacterium salinarum in a photobioreactor,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 1553–1562, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31014.