Hydrogen gas production using coupled enzyme systems from E-coli and Halobacterium halobium

Kaya, B
Gündüz, Ufuk
Eroğlu, İnci
Turker, L
Yucel, M
A photobioreactor containing enzyme systems from E.coli and Halobacterium halobium for hydrogen gas production was constructed. Bacteriorhodopsin is a retinal protein present in the purple membrane fragment of photosynthetic bacteria H. halobium which behaves as a light driven proton pump upon illumination. The H+ ion gradient produced between interior and exterior of the cell membrane utilized by the bacterial cell for synthesis of ATP as an energy source for its growth and survival. Under anaerobic growth conditions, the salt resistant E.coli DS 1576 induces the over expression of hydrogenase isoenzymes which catalyzes the reduction H+ ion to H-2 gas.


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Guler, Melih; Doğu, Timur; VARIŞLI, DİLEK (2017-12-15)
Ammonia decomposition reaction to produce COx free hydrogen over molybdenum incorporated mesoporous carbon based catalysts was studied in a microwave heated reactor system. Mesoporous carbon is acting as the catalyst support as well as the microwave receptor in the reaction medium. While the highest conversion value was 49% in conventionally heated reaction system at 600 degrees C, total conversion value was achieved over the same catalyst at 400 degrees C in microwave heated system with a GHSV of 36,000 ml...
Hydrogen gas production by electrohydrolysis of volatile fatty acid (VFA) containing dark fermentation effluent
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Hydrogen gas production by combined systems of Rhodobacter sphaeroides OU001 and Halobacterium salinarum in a photobioreactor
Zabut, Baker; EI-Kahlout, Kamal; Yucel, Meral; Gündüz, Ufuk; Turker, Lemi; Eroglu, Inci (2006-09-01)
Rhodobacter sphaeroides O.U.001 is a photosynthetic non-sulfur bacterium which produces hydrogen from organic compounds under anaerobic conditions. Halobacterium salinarum is an archaeon and lives under extremely halophilic conditions (4 M NaCl). H. salinarum contains a retinal protein bacteriorhodopsin in its purple membrane which acts as a light-driven proton pump. In this study the Rhodobacter sphaeroides O.U.001 culture was combined with different amounts of packed cells of H. salinarum S9 or isolated p...
Microwave-assisted ammonia decomposition reaction over iron incorporated mesoporous carbon catalysts
VARIŞLI, DİLEK; Korkusuz, Cansu; Doğu, Timur (2017-02-01)
Microwave-assisted ammonia decomposition reaction was investigated to produce COx free hydrogen, for fuel cell applications. Iron incorporated mesoporous carbon catalysts were prepared at different metal loadings, following an impregnation procedure. Mesoporous carbon acted as the catalyst support, as well as the microwave receptor. Complete conversion of ammonia was achieved at 450 degrees C over the catalyst having 7.7 wt% Fe, when the reaction was carried out in the microwave reactor system, using pure a...
Photoelectrochemical hydrogen production by Halobacterium halobium
Sediroglu, V; Yucel, M; Gunduz, L; Turker, L; Aydemir, A; Eroğlu, İnci (1996-06-28)
A bio-photoelectrochemical reactor has been designed and constructed in order to understand the biocatalytic effect of Halobacterium halobium on the electrochemical production of hydrogen in salt solution. Photoelectrochemical studies were carried out at 25 degrees C in 20 % NaCl solution containing 0.68 mu M bacteriorhodopsin. The reactor was illuminated by a 1000 W projector lamp which was 50 cm apart.
Citation Formats
B. Kaya, U. Gündüz, İ. Eroğlu, L. Turker, and M. Yucel, “Hydrogen gas production using coupled enzyme systems from E-coli and Halobacterium halobium,” 1996, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/52653.