Effect of ozone pretreatment on methane production and microbial community structure of single-stage anaerobic digesters

Tuncay, Sera
Increasing amount of sludge produced by wastewater treatment plants poses many problems in environmental, public health and economic aspects. Anaerobic digestion (AD), the most common sludge stabilization method, is used to reveal energy potential of sludge as methane-rich biogas. Various pretreatment methods are applied to enhance AD efficiency by facilitating the rate limiting hydrolysis step. Ozone pretreatment is a remarkable method for improving methane production, sludge reduction and pathogen removal. Therefore, this study investigated the effect of ozone pretreatment with varying doses of 0.03, 0.06 and 0.09 g O3/g TSS on methane production and microbial communities of mesophilic single-stage anaerobic digesters operated as semi-batch. Anaerobic digesters were monitored in terms of pH, temperature, chemical oxygen demand, total nitrogen, solids content, total volatile fatty acids, methane production and microbial community structure. Changes in methane and microbial community structure were determined by gas chromatography and fluorescence in situ hybridization (FISH) methods, respectively. Pretreatment with 0.06 g O3/g TSS resulted in 47% methane increase by achieving the highest methane content of 78%. FISH analyses revealed that Methanosaeta spp. were the most dominant methanogens in this pretreated digester.


Effect of ozone pretreatment on biogas production and microbial community structure of two-stage anaerobic digesters
Akçakaya, Merve; İçgen, Bülent; Department of Environmental Engineering (2021-2-09)
The amount of sludge produced in treatment plants is increasing depending on the population growth and water consumption. Anaerobic digestion (AD) is a widely used sludge stabilization method in wastewater treatment plants, as it provides volume reduction and methane-rich biogas production. In single-stage anaerobic digestions, the process fails due to volatile fatty acid accumulation and pH changes. To solve this problem, two-stage anaerobic digestion (TSAD) process has emerged t...
Effects of anaerobic digestion on the combustion of sewage sludge
Çelebi, Emrehan Berkay; Aksoy, Ayşegül; Sanin, Faika Dilek (2016-06-10)
Around the world, especially in the developing countries, sludge management is a topic gaining increasing importance, as the number of treatment plants and the people connected to these plants are on the rise. Old approaches seeing sludge as a waste, including landfilling, leave its place for new applications where sludge acts as an energy source or a resource as fertilizer. Combustion or co-combustion of sludge exploits its energy potential while providing stabilization ...
Behavior of sulfate-reducing bacteria in single-stage anaerobic digesters pretreated with ozone
Tuncay, Sera; Akcakaya, Merve; İçgen, Bülent (null; 2019-10-28)
Anaerobic digestion is a solid stabilization process commonly used at both municipal and industrial wastewater and sludge treatment. Anaerobic digesters convert the microbiological cells and other solids generated during the treatment process to a stable end product and also produce methane-rich biogas as an energy source through the degradation of organic matter. Microbial population dynamics involved in anaerobic digestion are not well-studied yet due to the requirement of an extensive investigation of mi...
Influence of Farming Intensity and Climate on Lowland Stream Nitrogen
Goyenola, Guillermo; Graeber, Daniel; Meerhof, Mariana; Jeppesen, Erik; Teixeira-de Mello, Franco; Vidal, Nicolás; Fosalba, Claudia; Ovesen, Niels Bering; Gelbrecht, Joerg; Mazzeo, Néstor; Kronvang, Brian (MDPI AG, 2020-4-2)
Nitrogen lost from agriculture has altered the geochemistry of the biosphere, with pronounced impacts on aquatic ecosystems. We aim to elucidate the patterns and driving factors behind the N fluxes in lowland stream ecosystems differing about land-use and climatic-hydrological conditions. The climate-hydrology areas represented humid cold temperate/stable discharge conditions, and humid subtropical climate/flashy conditions. Three complementary monitoring sampling characteristics were selected, including a ...
Formation of nitrogen functionalities in biochar materials and their role in the mitigation of hazardous emerging organic pollutants from wastewater
Başer, Begüm; Yousaf, Balal; Yetiş, Ülkü; Abbas, Qumber; Kwon, Eilhann E.; Wang, Shengsen; Bolan, Nanthi S.; Rinklebe, Jörg (2021-08-15)
Emerging organic pollutants (EOPs) are serious environmental concerns known for their prominent adverse and hazardous ecological effects, and persistence in nature. Their detrimental impacts have inspired researchers to develop the strategic tools that reduce and overcome the challenges caused by EOPs' rising concentration. As such, biochar becomes as a promising class of biomass-derived functional materials that can be used as low-cost and environmentally-friendly emerging catalysts to remove EOPs. Herein,...
Citation Formats
S. Tuncay, “Effect of ozone pretreatment on methane production and microbial community structure of single-stage anaerobic digesters,” M.S. - Master of Science, Middle East Technical University, 2021.