Ozonation of sewage sludge prior to anaerobic digestion led to Methanosaeta dominated biomethanation

Tuncay, Sera
Akcakaya, Merve
İçgen, Bülent
Anaerobic digestion (AD) of sewage sludge is commonly employed for sludge stabilization and energy generation at wastewater treatment plants. Of pretreatment techniques used to accelerate rate-limiting hydrolysis step in AD, economically feasible ozonation is prevalent and permits sludge reduction via oxidation. The stability of AD process during ozonation is crucial for successful energy recovery and needs optimal dosage that encourages methanogenic consortium efficient in methane production. This study, therefore, aimed at investigating the impact of 0.03, 0.06 and 0.09 g O3/g TSS ozone doses on the productivity of mesophilic semi-batch lab-scale 5 L AD operated at a hydraulic retention time of 15 d with organic loading rates of 1.45–1.80 g VS/L.d and on its methanogenic consortium. Measurements of biogas, methane and quantification of methanogenic consortium were done by water displacement, gas chromatography, and fluorescence in situ hybridization techniques, respectively. Although all applied ozone doses improved sludge characteristics, the highest average daily removal efficiencies with 35% COD, 32% TS, 42% VS, 60% TSS and 69% VSS were achieved through ozonation with 0.06 g O3/g TSS ozone dose. The same ozone dose also yielded 48% more methane in the biogas recovered and this biomethanation was correlated to the abundance of acetotrophic Methanosaeta species.


Behavior of sulfate-reducing bacteria in single-stage anaerobic digesters pretreated with ozone
Tuncay, Sera; Akcakaya, Merve; İçgen, Bülent (null; 2019-10-28)
Anaerobic digestion is a solid stabilization process commonly used at both municipal and industrial wastewater and sludge treatment. Anaerobic digesters convert the microbiological cells and other solids generated during the treatment process to a stable end product and also produce methane-rich biogas as an energy source through the degradation of organic matter. Microbial population dynamics involved in anaerobic digestion are not well-studied yet due to the requirement of an extensive investigation of mi...
Effect of ozone pretreatment on methane production and microbial community structure of single-stage anaerobic digesters
Tuncay, Sera; İçgen, Bülent; Department of Environmental Engineering (2021-2-09)
Increasing amount of sludge produced by wastewater treatment plants poses many problems in environmental, public health and economic aspects. Anaerobic digestion (AD), the most common sludge stabilization method, is used to reveal energy potential of sludge as methane-rich biogas. Various pretreatment methods are applied to enhance AD efficiency by facilitating the rate limiting hydrolysis step. Ozone pretreatment is a remarkable method for improving methane production, sludge reduction and pathogen removal...
YURTERI, C; GUROL, MD (1990-06-01)
This paper is focused on the use of ozone for the elimination of manmade organic micropollutants from drinking waters and waste effluents requiring advanced treatment. A mathematical model was developed to simulate the physical transport and chemical oxidation phenomena prevailing during the process of ozonation. A packed column reactor was constructed in order to test the aptness of the process model. After determining the fluid-dynamic and mass transfer properties of the reactor, stock water solutions spi...
Disintegration of Sludge by Sonication and Improvement of Methane Production Rates in Batch Anaerobic Digesters
Zorba, Gozde T.; Sanin, Faika Dilek (2013-04-01)
Sludge pretreatment prior to anaerobic digestion has been found to reduce sludge production in wastewater treatment. Sludge disintegration using physical, chemical, biological, or mechanical methods can increase biogas production and reduce sludge quantities. Ultrasonication is one of the most effective means of mechanical disintegration. This study aims to investigate ultrasonication as a means for solubilizing waste activated sludge (WAS) to enhance its digestability. Sonication was applied by the use of ...
Two-stage anaerobic digestion of ozonated sewage sludge predominantly took over by acetotrophic methanogens with increased biogas and methane production
Akcakaya, Merve; Tuncay, Sera; İçgen, Bülent (2022-06-01)
Ozonation of sewage sludge prior to single-stage anaerobic digestion (SAD) has been reported to increase biogas and/or methane production. However, no studies have evaluated the impact of ozone pre-treatment on two-stage anaerobic digestion (TAD) of sewage sludge over the production of these renewable fuels. Present study, therefore, aimed at investigating the impact of 0.06 g O3/g TSS ozone-pretreated sewage sludge on the productivity of mesophilic semi-batch lab-scale TAD and on its methanogenic consortiu...
Citation Formats
S. Tuncay, M. Akcakaya, and B. İçgen, “Ozonation of sewage sludge prior to anaerobic digestion led to Methanosaeta dominated biomethanation,” Fuel, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/94945.