Evaluating Oxygen Tensions Related to Bone Marrow and Matrix for MSC Differentiation in 2D and 3D Biomimetic Lamellar Scaffolds

Sayin, Esen
Baran, Erkan Turker
Elsheikh, Ahmed
Mudera, Vivek
Cheema, Umber
Hasırcı, Vasıf Nejat
The physiological O-2 microenvironment of mesenchymal stem cells (MSCs) and osteoblasts and the dimensionality of a substrate are known to be important in regulating cell phenotype and function. By providing the physiologically normoxic environments of bone marrow (5%) and matrix (12%), we assessed their potential to maintain stemness, induce osteogenic differentiation, and enhance the material properties in the micropatterned collagen/silk fibroin scaffolds that were produced in 2D or 3D. Expression of osterix (OSX) and vascular endothelial growth factor A (VEGFA) was significantly enhanced in the 3D scaffold in all oxygen environments. At 21% O-2, OSX and VEGFA expressions in the 3D scaffold were respectively 13,200 and 270 times higher than those of the 2D scaffold. Markers for assessing stemness were significantly more pronounced on tissue culture polystyrene and 2D scaffold incubated at 5% O-2. At 21% O-2, we measured significant increases in ultimate tensile strength (p < 0.0001) and Young's modulus (p = 0.003) of the 3D scaffold compared to the 2D scaffold, whilst 5% O-2 hindered the positive effect of cell seeding on tensile strength. In conclusion, we demonstrated that the 3D culture of MSCs in collagen/silk fibroin scaffolds provided biomimetic cues for bone progenitor cells toward differentiation and enhanced the tensile mechanical properties.


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Cell and substrate interactions are important in tissue engineering products especially on the behavior of the cells such as adhesion, migration, proliferation, and differentiation. These have been widely studied using substrates with different physical, chemical, and mechanical properties and form. In this study, elastin-like recombinamers (ELRs) were used blended with collagen or only collagen as the surface material. The ELR used in this study has Valine-Proline-Glycine-X-Glycine aminoacid sequences in i...
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Mesenchymal stem cells (MSCs) are promising cell sources for tissue engineering applications as they can differentiate into a variety of adult cells types including osteoblasts. In vivo microenvironment of stem cells is known to provide both biochemical signals and micro- and nanoscale physical cues that influence the behavior and fate of stem cells. The use of soluble chemical factors is the most common strategy to guide the commitment of MSCs to specific lineages, but it is a cause of concern such as unsa...
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Various physiological and pathological processes, such as cell differentiation, migration, attachment, and metastasis are highly dependent on nuclear elasticity. Nuclear morphology directly reflects the elasticity of the nucleus. We propose that quantification of changes in nuclear morphology on surfaces with defined topography will enable us to assess nuclear elasticity and deformability. Here, we used soft lithography techniques to produce 3 dimensional (3-D) cell culture substrates decorated with micron ...
Citation Formats
E. Sayin, E. T. Baran, A. Elsheikh, V. Mudera, U. Cheema, and V. N. Hasırcı, “Evaluating Oxygen Tensions Related to Bone Marrow and Matrix for MSC Differentiation in 2D and 3D Biomimetic Lamellar Scaffolds,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/90365.