Selecting 2-FY RNA Aptamers Against SARS-CoV-2 Particle

Öztürk, Meriç
Corona Virus Disease – 19 (COVID-19) is caused by infection of SARS-CoV-2 from other humans and animals, which results in clinical symptoms like fever, cough, breathing difficulties headache, muscle pain, and diarrhea. Further symptoms can be life threatening clinical conditions such as pneumonia, cardiovascular and rarely neurological complexities. According to studies, estimated fatality rate of the disease is about 4 %; reported cases-fatality rate is about 2.3 %. Early diagnosis is crucial to prevent further fatal symptoms. Although PCR tests are widely used, their success rate is about 60% and getting results of the test can take hours to days. Thus, more effective, and faster diagnostic tool is required. Aptamers are small nucleic acids that can be selected in vitro bind to their cognate targets with a high specificity and affinity. They can have either ssDNA or RNA backbones and they fold naturally into their unique three-dimensional shape. Aptamers can also be further modified to enhance their structural stability without any loss of affinity. These properties make them useful for both therapeutic and diagnosis applications. In this study, we successfully generated SARS-CoV-2 binding aptamers that will be further optimized for COVID-19 diagnosis.


ARDIÇ, FUNDA ZEKİYE; Gözen, Ayşe Gül; İlgü, Müslüm; Department of Molecular Biology and Genetics (2022-2-11)
Crimean-Congo hemorrhagic fever (CCHF) is potentially lethal and mostly infected by ticks. CCHF virus (CCHFV), is a Nairoviridae family member of the Orthonairovirus genus and in general, cases are reported from Asia, Africa, the Middle East, and Eastern Europe. The fatality rate ranges from 30 to 50% worldwide but it is reported as 5% by the Turkish Ministry of Health and it varies between 1.7 and 26.5% for children. ELISA kits used for the detection of infection are time-consuming, expensive, and labor-in...
Characterization of leishmania RNA virus infected leishmania major exosomes and evaluation of the impact of cytosolic DNA sensing related pathways in L. Major infection
Yılmaz, İsmail Cem; Gürsel, Mayda; Department of Biology (2020)
Leishmaniasis is a neglected infectious disease caused by Leishmaniaparasite. The disease represents a significant health problem, necessitating urgent development of effective treatment strategies. New World Leishmaniaspecies harboringLeishmania RNA virus (LRV) causesevere,metastatic disease. In this thesis, we compared LRV2-1 deficient and proficient Old World L. majorstrains in terms of their exosomal content and infectivity. PCR and mass spectrometry based analysis revealed that virus infectedLeishm...
A Novel Therapeutic Peptide Blocks SARS-CoV-2 Spike Protein Binding with Host Cell ACE2 Receptor
Rajpoot, Sajjan; Ohishi, Tomokazu; Kumar, Ashutosh; Pan, Qiuwei; Banerjee, Sreeparna; Zhang, Kam Y. J.; Baig, Mirza S. (2021-07-01)
Background and Objective Coronavirus disease 2019 is a novel disease caused by the severe acute respiratory syndrome coronavirus (SARS-CoV)-2 virus. It was first detected in December 2019 and has since been declared a pandemic causing millions of deaths worldwide. Therefore, there is an urgent need to develop effective therapeutics against coronavirus disease 2019. A critical step in the crosstalk between the virus and the host cell is the binding of the receptor-binding domain (RBD) of the SARS-CoV-2 spike...
Expanding the Clinical and Immunological Phenotypes and Natural History of MALT1 Deficiency
Sefer, Asena Pinar; et. al. (2022-01-01)
Purpose MALT1 deficiency is a combined immune deficiency characterized by recurrent infections, eczema, chronic diarrhea, and failure to thrive. Clinical and immunological characterizations of the disease have not been previously reported in large cohorts. We sought to determine the clinical, immunological, genetic features, and the natural history of MALT-1 deficiency. Methods The clinical findings and treatment outcomes were evaluated in nine new MALT1-deficient patients. Peripheral lymphocyte subset anal...
Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis
Koc, Dilara; Begentaş, Onur Can; Yurtogullari, Sukran; Temel, Musa; Akcali, Kamil Can; Demirkaya, Seref; Kiriş, Erkan (2021-05-01)
Multiple sclerosis is a chronic disease characterized by inflammation, demyelination, and axonal damage in the central nervous system. Here, we established an induced pluripotent stem cell (iPSC) line METUi001-A from the peripheral blood mononuclear cells of a 25-year-old male individual with clinically diagnosed Relapsing-Remitting Multiple Sclerosis (RRMS) using the integration-free Sendai reprogramming method. We demonstrated that the iPSCs are free of exogenous Sendai reprogramming vectors, have a norma...
Citation Formats
M. Öztürk, “Selecting 2-FY RNA Aptamers Against SARS-CoV-2 Particle,” M.S. - Master of Science, Middle East Technical University, 2021.