SELECTION OF 2'FY RNA APTAMERS AGAINST CRIMEAN CONGO HAEMORRHAGIC FEVER VIRUS

Download
2022-2-11
ARDIÇ, FUNDA ZEKİYE
Crimean-Congo hemorrhagic fever (CCHF) is potentially lethal and mostly infected by ticks. CCHF virus (CCHFV), is a Nairoviridae family member of the Orthonairovirus genus and in general, cases are reported from Asia, Africa, the Middle East, and Eastern Europe. The fatality rate ranges from 30 to 50% worldwide but it is reported as 5% by the Turkish Ministry of Health and it varies between 1.7 and 26.5% for children. ELISA kits used for the detection of infection are time-consuming, expensive, and labor-intensive; and because ELISAs detecting IgG and IgM antibodies are produced against virus, and don’t specifically detect virus presence; these techniques may cause some inaccurate diagnosis and also they have no reusability. The diagnosis cost will be a lot higher when considering the cost of extra microbiological tests for infectious diseases (Viral hepatitis, typhoid, leptospirosis and Hantavirus infectious, etc.) that are misdiagnosed with CCHF. As a result, a more effective and faster diagnostic technology is required. Aptamers are tiny nucleic acids which can be selected in vitro and have a high affinity and selectivity for their relevant targets. They can contain ssDNA or RNA backbones, and they fold into their three-dimensional structure naturally. Aptamers can also be changed to improve their structural stability while maintaining their affinity. Because of these characteristics, they can be used for both therapeutic and diagnostic purposes. CCHFV binding aptamers were successfully selected in this study, and they will be further optimized for CCHF diagnosis.

Suggestions

Selecting 2-FY RNA Aptamers Against SARS-CoV-2 Particle
Öztürk, Meriç; Gözen, Ayşe Gül; İlgü, Müslüm; Department of Molecular Biology and Genetics (2021-6-22)
Corona Virus Disease – 19 (COVID-19) is caused by infection of SARS-CoV-2 from other humans and animals, which results in clinical symptoms like fever, cough, breathing difficulties headache, muscle pain, and diarrhea. Further symptoms can be life threatening clinical conditions such as pneumonia, cardiovascular and rarely neurological complexities. According to studies, estimated fatality rate of the disease is about 4 %; reported cases-fatality rate is about 2.3 %. Early diagnosis is crucial to prevent fu...
Systematic evolution of novel 2′F-PY RNA aptamers targeting the membrane protein l-arginine/agmatine antiporter purified in mild detergent
Ayoub, Nooraldeen; Son, Çağdaş Devrim; İlgü, Müslüm; Department of Biochemistry (2021-2-15)
Bacterial microorganisms have developed numerous and diverse systems to handle potentially detrimental acidic conditionsintheir external environment. In particular, some pathogenic and other nonpathogenic enteric bacteriahave a number of exceptionaland well-organized acid resistance (AR) mechanismsthat work together to counter intracellular acidification and damage and enable survival under the extreme acidic conditions of the mammalian stomach. The common Escherichia coliwith both its virulent ...
Inhibition of the TIRAP-c-Jun interaction as a therapeutic strategy for AP1-mediated inflammatory responses
Srivastava, Mansi; Saqib, Uzma; Banerjee, Sreeparna; Wary, Kishore; Kizil, Burak; Muthu, Kannan; Baig, Mirza S. (Elsevier BV, 2019-06-01)
Bacterial endotoxin-induced sepsis causes 30-40% of the deaths in the intensive care unit (ICU) globally, for which there is no pharmacotherapy. Lipopolysaccharide (LPS), a bacterial endotoxin, stimulates the Toll-like receptor (TLR)-4 signalling pathways to upregulate the expression of various inflammatory mediators. Here, we show that the TIRAP and c-Jun protein signalling complex forms in macrophages in response to LPS stimulation, which increases the AP1 transcriptional activity, thereby amplifying the ...
Reconstruction of the temporal signaling network in Salmonella-infected human cells
Budak, Gungor; Ozsoy, Oyku Eren; Aydın Son, Yeşim; Can, Tolga; Tunçbağ, Nurcan (2015-07-20)
Salmonella enterica is a bacterial pathogen that usually infects its host through food sources. Translocation of the pathogen proteins into the host cells leads to changes in the signaling mechanism either by activating or inhibiting the host proteins. Given that the bacterial infection modifies the response network of the host, a more coherent view of the underlying biological processes and the signaling networks can be obtained by using a network modeling approach based on the reverse engineering principl...
Characterization and genomic analysis of a novel bacteriophage against methicillin-resistant staphylococcus aureus
Çotak, Medine; Akkaya, Mahinur S.; Karahan, Zeynep Ceren; Department of Biotechnology (2019)
Methicillin resistant Staphylococcus aureus (MRSA) is one of the most frequent hospital and community acquired infectious agents causing severe diseases. Bacteriophages may offer a solution to treat the bacterial infections that are not responding to classical antibiotics. In this context, a new lytic phage, named as vB_SauM-MikSA913, was isolated from sewage treatment center in Samsun (Turkey). MRSA clinical strains obtained from a local hospital were used as propagating hosts while searching for the lytic...
Citation Formats
F. Z. ARDIÇ, “SELECTION OF 2’FY RNA APTAMERS AGAINST CRIMEAN CONGO HAEMORRHAGIC FEVER VIRUS,” M.S. - Master of Science, Middle East Technical University, 2022.