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Silver nanowire loaded poly(ε-caprolactone) nanocomposite fibers as electroactive scaffolds for skeletal muscle regeneration
Date
2021-01-01
Author
Basturkmen, Berk
Ergene, Emre
Doğanay, Doğa
HURİ, PINAR
Ünalan, Hüsnü Emrah
AKSOY, EDA AYŞE
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Volumetric muscle loss (VML) due to trauma and tumor removal operations affects millions of people every year. Although skeletal muscle has a natural repair mechanism, it cannot provide self-healing above a critical level of VML. In this study, nanocomposite aligned fiber scaffolds as support materials were developed for volumetric skeletal muscle regeneration. For this purpose, silver nanowire (Ag NW) loaded poly(ε-caprolactone) (PCL) nanocomposite fiber scaffolds (PCL-Ag NW) were prepared to mimic the aligned electroactive structure of skeletal muscle and provide topographic and conductive environment to modulate cellular behavior and orientation. A computer-aided rotational wet spinning (RWS) system was designed to produce high-yield fiber scaffolds. Nanocomposite fiber bundles with lengths of 50 cm were fabricated via this computer-aided RWS system. The morphological, chemical, thermal properties and biodegradation profiles of PCL and PCL-Ag NW nanocomposite fibers were characterized in detail. The proliferation behavior and morphology of C2C12 mouse myoblasts were investigated on PCL and PCL-Ag NW nanocomposite fibrous scaffolds with and without electrical stimulation. Significantly enhanced cell proliferation was observed on PCL-Ag NW nanocomposite fibers compared to neat PCL fibers with electrical stimulations of 1.5 V, 3 V and without electrical stimulation.
Subject Keywords
Electrical stimulation
,
Nanocomposite fiber
,
Poly(ε-caprolactone)
,
Rotational wet spinning
,
Silver nanowire
,
Skeletal muscle tissue engineering
URI
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85120648139&origin=inward
https://hdl.handle.net/11511/95276
Journal
Materials Science and Engineering C
DOI
https://doi.org/10.1016/j.msec.2021.112567
Collections
Department of Metallurgical and Materials Engineering, Article
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B. Basturkmen, E. Ergene, D. Doğanay, P. HURİ, H. E. Ünalan, and E. A. AKSOY, “Silver nanowire loaded poly(ε-caprolactone) nanocomposite fibers as electroactive scaffolds for skeletal muscle regeneration,”
Materials Science and Engineering C
, pp. 0–0, 2021, Accessed: 00, 2022. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85120648139&origin=inward.