3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering

Download

10424195_BuşraYedekçi_PhD_tez.pdf

Date

2021-9-6

Author

Yedekçi, Buşra

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

436
views

302
downloads

Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped hydroxyapatite (HA) that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HAs were synthesized by microwave irradiation method and samples were sintered at 700, 900 and 1100°C. Microstructural, mechanical and biological characterization results were evaluated and 4 different groups sintered at 1100°C were selected for scaffold construction. Then, PCL-PEG-PCL copolymer was successfully synthesized with ring opening polymerization method and characterized by FTIR, 1H NMR and GPC analyses. PCL-PEG-PCL composite scaffolds containing different amounts of hydroxyapatite (HA) (10% and 20 wt%) were produced with the desired porosity (50% and 60%) by compression-molding/particulate leaching method. Presence of HA in the scaffolds improved the mechanical properties. Compressive strength of the scaffolds was between 9.32-24.27 MPa and 20% 2Sr0.5BHA scaffolds were found to have the maximum compressive strength. In the relative cell viability (%) test, the highest viability was observed on the scaffolds with HA and 2Sr0.5BHA. The specific ALP activity level of the cells on the scaffolds containing 2Sr0.5BHA was significantly higher (2.6 times) than that of the control group. It was concluded that PCL-PEG-PCL composite scaffolds with 2Sr0.5BHA have the potential to be used in BTE.

Subject Keywords

B-Sr-Mg multi-doped hydroxyapatite, PCL-PEG-PCL, Composite scaffolds, Bone tissue engineering

URI

https://hdl.handle.net/11511/93179

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

3D porous PCL-PEG-PCL / strontium, magnesium and boron multi-doped hydroxyapatite composite scaffolds for bone tissue engineering Yedekçi, Buşra; Tezcaner, Ayşen; Evis, Zafer (2022-01-01) Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped HA that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HA and PCL-PEG-PCL copolyme...
Preparation of Barium doped Baghdadite/PHBV fibrous scaffolds for bone tissue engineering Sadreddini, Sanaossadat; Evis, Zafer; Keskin, Dilek; Department of Biomedical Engineering (2023-1-23) Recently, bioceramic/polymer composites have dragged a lot of attention for treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop fibrous composite poly(hydroxybutyrate) co (hydroxyvalerate)- polycaprolactone, PHBV-PCL, scaffolds containing different amounts of baghdadite (BAG) and Ba-doped BAG that can provide bone regeneration in the bone defect area and to investigate the effect of these scaffolds on the structural, mechanical, and biological propertie...
Development of manganese-doped hydroxyapatite incorporated PCL electrospun 3D scaffolds coated with gelatin for bone tissue engineering Samiei, Alaleh; Keskin, Dilek; Evis, Zafer; Department of Biomedical Engineering (2023-1-27) Combination of polymers and bioceramics has increased their importance in bone tissue engineering (BTE) to treat various defects. Within this frame, in this thesis, it is aimed to develop a 3D gelatin-coated PCL scaffold combined with Mn-doped hydroxyapatite (HA) in order to investigate the effect of the doping element, i.e., the manganese (Mn) ion, on the structural and biological properties of the composite scaffold. Pure and Mn-doped HAs were synthesized using microwave irradiation, and the samples were ...
3D porous bioceramic based boron-doped hydroxyapatite/baghdadite composite scaffolds for bone tissue engineering Jodati, Hossein; Evis, Zafer; Tezcaner, Ayşen; Alshemary, Ammar Z.; Motameni, Ali (2023-04-01) Making composite scaffolds is one of the well-known methods to improve the properties of scaffolds used in bone tissue engineering. In this study, novel ceramic-based 3D porous composite scaffolds were successfully prepared using boron-doped hydroxyapatite, as the primary component, and baghdadite, as the secondary component. The effects of making composites on the properties of boron-doped hydroxyapatite-based scaffolds were investigated in terms of physicochemical, mechanical, and biological properties. T...
Surface functionalized poly-lactic acid (PLA) scaffolds for bone tissue engineering Monirizad, Mahsa; Keskin, Dilek; Ermiş Şen, Menekşe; Department of Engineering Sciences (2022-2) The need for more effective tissue grafts for orthopedic applications is one of the main research areas of tissue engineering. In bone tissue engineering (BTE), scaffolds that can mimic bone tissue both from mechanical and biological perspectives are investigated mostly. In this study, it was aimed to develop a BTE scaffold that can mimic bone ECM, mechanical strength and cell biocompatibility in a single design and thus, various groups of scaffolds were characterized in terms of mechanical, biocompatibilit...

Citation Formats

B. Yedekçi, “3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering,” Ph.D. - Doctoral Program, Middle East Technical University, 2021.