3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering

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2021-9-6

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Yedekçi, Buşra

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Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped hydroxyapatite (HA) that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HAs were synthesized by microwave irradiation method and samples were sintered at 700, 900 and 1100°C. Microstructural, mechanical and biological characterization results were evaluated and 4 different groups sintered at 1100°C were selected for scaffold construction. Then, PCL-PEG-PCL copolymer was successfully synthesized with ring opening polymerization method and characterized by FTIR, 1H NMR and GPC analyses. PCL-PEG-PCL composite scaffolds containing different amounts of hydroxyapatite (HA) (10% and 20 wt%) were produced with the desired porosity (50% and 60%) by compression-molding/particulate leaching method. Presence of HA in the scaffolds improved the mechanical properties. Compressive strength of the scaffolds was between 9.32-24.27 MPa and 20% 2Sr0.5BHA scaffolds were found to have the maximum compressive strength. In the relative cell viability (%) test, the highest viability was observed on the scaffolds with HA and 2Sr0.5BHA. The specific ALP activity level of the cells on the scaffolds containing 2Sr0.5BHA was significantly higher (2.6 times) than that of the control group. It was concluded that PCL-PEG-PCL composite scaffolds with 2Sr0.5BHA have the potential to be used in BTE.

Subject Keywords

B-Sr-Mg multi-doped hydroxyapatite, PCL-PEG-PCL, Composite scaffolds, Bone tissue engineering

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https://hdl.handle.net/11511/93179

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

B. Yedekçi, “3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering,” Ph.D. - Doctoral Program, Middle East Technical University, 2021.