Bacterial cellulose-reinforced boron-doped hydroxyapatite/gelatin scaffolds for bone tissue engineering

Date

2019-12-01

Author

Atila, Deniz
Karataş, Ayten
Evcin, Atilla
Keskin, Dilek
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

202
views

0
downloads

Today, many people are suffering from bone defects due to trauma, tumor or bone related diseases. Mimicking bone in terms of composition and structure has been a challenge for tissue engineers. In this study, a novel 3D porous tissue-engineered construct composed of natural and easily-accessible biomaterials, namely (1) an exopolysaccharide; bacterial cellulose (BC), (2) mineral crystals; boron-doped nano-hydroxyapatite (BHA), and (3) a natural protein; gelatin (GEL) as the main constituting biomaterial was prepared by a simple and cost-effective technique; lyophilization, for bone tissue engineering applications. BC was produced by Gluconacetobacter xylinum bacteria species and hydroxyapatite (HA) and BHA were synthesized via sol-gel technique. Characterizations of GEL-BC, GEL-BC/HA, and GEL-BC/BHA scaffolds showed that they all possessed porous structure and pores became more irregular with the addition of HA or BHA. Scaffolds exhibited high water absorption, suitable degradation rate while having in vitro bioactivity with a Ca/P ratio similar to that of bone (Ca/P < 1.67). Thermo-gravimetric analysis showed that structural stability of the scaffolds was improved with the addition of HA and BHA. The porosity of scaffolds was similar (68.49-80.94%). HA and BHA-incorporation into scaffolds further improved the mechanical properties. Cell culture studies conducted with Saos-2 cell line showed that cells attached, proliferated on the GEL-BC/BHA scaffolds at a higher level demonstrating that the scaffolds were cytocompatible. ALP activity of cells seeded on GEL-BC/BHA scaffolds was statistically highest at day 14 which was also correlated with the results of intracellular calcium deposition. Thus, GEL-BC/BHA scaffolds hold potential for use in bone tissue engineering applications.

Subject Keywords

Polymers and Plastics

URI

https://hdl.handle.net/11511/48026

Journal

CELLULOSE

DOI

https://doi.org/10.1007/s10570-019-02741-1

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Preparation and characterization of acrylic bone cements Endoğan, Tuğba; Hasırcı, Nesrin; Department of Chemistry (2005) Acrylic bone cements are used in dentistry and orthopedic surgery to fix prosthetic devices into the bone. Bone cements transfer and distribute the applied load and increase the load-carrying capacity of the prosthesis/cement/bone system with the help of mechanical bonding between the device and the bone. In spite of all their advantages, bone cements have several drawbacks such as insufficient mechanical properties, high exothermic polymerization temperature, release of monomer to the environmental tissue ...
Biodegradable polymer - hydroxyapatite nanocomposites for bone plate applıcations Aydın, Erkin; Hasırcı, Vasıf Nejat; Planell, Joseph A.; Department of Biotechnology (2010) Long bone fractures are fixed with bone plates to restrain movement of bone fragments. Fracture site must experience some pressure for proper healing. Bone plates are mostly made up of metals having 5 - 10 times higher elastic modulus than bones and most of the load is carried by them, leading to stress shielding and a bony tissue with low mineral density and strength. To avoid these problems, biodegradable polymer-based composite plates were designed and tested in this study. Poly(L-lactide) and Poly(3-hyd...
Design of a modular orthopedic implant Erkan, Onur Mert; Tönük, Ergin; Department of Mechanical Engineering (2015) Bone fracture due to trauma and bone defects by birth are very common in orthopedics, making their treatment crucial. In this study, a novel design to treat upper arm fractures is introduced and assessed mechanically. The design offers medical doctors longitudinal and angular flexibility when compared to widely used orthopedic plates. Hence the new design covers a variety of fracture types. Earlier conceptual designs are reviewed to demonstrate the progress of design. Mechanical performance of the final des...
Biodegradable hydroxyapatite - Polymer composites Durucan, Caner (2001-04-01) The fracture of bone due to trauma or due to natural aging is one of the most frequent types of tissue failures. Treatment frequently requires the implantation of ct temporary or permanent prosthesis. The implanted materials may include the components of artificial joints, plates, and screws for fracture fixation. Typically, such implants are intended only to provide structural support or to serve as templates for bone re-growth. In general they are intended to remain in place for the life of the patient or...
Development of a siRNA delivery system for the treatment of osteoporosis Sezlev Bilecen, Deniz; Hasırcı, Vasıf Nejat; Uludağ, Hasan; Department of Biotechnology (2018) Osteoporosis, the most common disease of bone, is a skeletal disorder associated with low bone mass, increase in bone fragility and in susceptibility to fractures. The high bone resorption rate is shown to be due to increased number and activity of the osteoclasts. Receptor Activator of Nuclear Factor kappa B (RANK)/ Receptor Activator of Nuclear Factor kappa B Ligand (RANKL) system plays a crucial role in osteoclast differentiation and bone remodeling. RANKL participates in differentiation and activation o...

Citation Formats

D. Atila, A. Karataş, A. Evcin, D. Keskin, and A. Tezcaner, “Bacterial cellulose-reinforced boron-doped hydroxyapatite/gelatin scaffolds for bone tissue engineering,” CELLULOSE, pp. 9765–9785, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48026.