Formulation of apple pomace, orange peel, and hazelnut shell mix for co-production of bacterial pectinase and cellulase enzymes by mixture design method

Ozzeybek, Mehlika
Çekmecelioğlu, Deniz
The cost of enzyme is high due to mostly pure carbon and nitrogen sources. Thus, there is a still need for exploring alternative feedstock to enhance enzyme production and reduce the cost. In this study, proportions of apple pomace, orange peels, and hazelnut shells were formulated by the extreme vertices mixture design (EVMD) method in an attempt to enhance co-production of bacterial pectinase and cellulase enzymes in comparison to production medium with single feedstock. Bacillus subtilis was grown in fermentation media containing acid-pretreated feedstock and minerals at initial pH of 7.0 and 30 degrees C for 72 h. Analysis of results from EVMD resulted in a mix of 20% apple pomace, 30% orange peels, and 50% hazelnut shells as optimal, which provided 8.27 U/mL of pectinase and 0.5 U/mL of cellulase under experimentation. These results implied 40% increase in pectinase but no change in cellulase production compared to the pectinase (5.93 U/mL) and cellulase (0.49 U/mL) obtained with hazelnut shell hydrolysate medium. It was also found that hazelnut shells gave slightly higher pectinase than apple pomace and orange peel media. This study proves the use of food processing wastes in mixed form rather than separate feedstock for enhancing pectinase production.


Formulation of food processing wastes for co-production of bacterial pectinase and cellulase enzymes
Özzeybek, Mehlika; Çekmecelioğlu, Deniz; Department of Food Engineering (2022-5-16)
Production of enzymes has always been the focus of research with its ever-increasing application area, yet it is an expensive process due to the need for pure carbon sources to carry out the production. Exploring new ways to reduce cost while maximizing enzyme production is an ongoing battle, even in today's opportunities. In this research, a mixture of food wastes was valorized and bacterial pectinase and cellulase were co-produced. Optimum proportions of the hazelnut shells, orange peel, and apple pomace ...
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Optically pure secondary alcohols are important building blocks for the synthesis of chemicals used in pharmaceutical sciences. Kinetic resolution of secondary alcohols through selective oxidation of one enantiomer to the corresponding ketone is a classical and efficient method to obtain enantiopure alcohols. An oxidative kinetic resolution is achieved by exploiting two enantiomers’ unequal rates of oxidation reaction with a chiral reagent or catalyst. The general approach towards a kinetic resolution via a...
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Citation Formats
M. Ozzeybek and D. Çekmecelioğlu, “Formulation of apple pomace, orange peel, and hazelnut shell mix for co-production of bacterial pectinase and cellulase enzymes by mixture design method,” BIOMASS CONVERSION AND BIOREFINERY, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: