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Cloning and screening of the putative hexokinase genes from Rhizopus oryzae and their heterologous expression in Saccharomyces cerevisiae
Date
2022-06-01
Author
Alagoz, Eda
Acar, Seyda
Yucel, Meral
Hamamcı, Haluk
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Background A filamentous fungus, Rhizopus oryzae (R. oryzae) is one of the ideal candidates for ethanol and lactic acid production due to its ability to grow on renewable carbon sources.
Subject Keywords
Saccharomyces cerevisiae
,
Rhizopus oryzae
,
Hexokinase
,
Lactic acid
,
Cloning
,
Transformation
,
BIOCHEMICAL-CHARACTERIZATION
,
ASPERGILLUS-NIGER
,
TREHALOSE-6-PHOSPHATE SYNTHASE
,
YEAST
,
GLUCOKINASE
,
GLYCOLYSIS
,
PURIFICATION
,
REPRESSION
,
ACID
URI
https://hdl.handle.net/11511/99197
Journal
MOLECULAR BIOLOGY REPORTS
DOI
https://doi.org/10.1007/s11033-022-07696-4
Collections
Department of Food Engineering, Article
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Revealing the hexokinase step of glycolysis in lactic acid producer fungus Rhizopus oryzae
Alagoz, Eda; Acar, Seyda; Yucel, Meral; Hamamcı, Haluk (2012-09-23)
Rhizopus oryzae is a filamentous fungus which can grow on some renewable carbon sources and this makes it a good candidate for production of lactic acid and ethanol. However, in R. oryzae the production yields of these products are lower compared to alternative organisms. The utilization of the carbon source through glycolysis might end up in lactic acid or ethanol production. This makes the glycolytic pathway a focus of attention for our search for a way to increase the speed and efficiency of this pathway...
Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae
Uyar, Ebru Ozer; Yucel, Meral; Hamamcı, Haluk (2016-05-01)
Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8kDa. The amino acid sequence of RoTPS1 has a relatively high homology with th...
Screening of the putative hexokinase genes from rhizopus oryzae
Alagöz, Eda; Hamamcı, Haluk; Yücel, Ayşe Meral; Department of Biotechnology (2013)
Rhizopus oryzae is a filamentous fungus which can ferment sugar to ethanol, and lactic acid. Increasing demand of the world for use of renewable carbon sources has put this and similar organisms into a position where the biotechnology industries are more and more interested with these organisms. R. oryzae can grow on some renewable carbon sources which makes it a good candidate for production of ethanol as well as lactic acid. The major use of lactic acid is in food industry and food- related application, w...
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Rhizopus oryzae is a filamentous fungus, which can produce high amounts of L(+)-lactic acid and produces ethanol as the main by-product. In an effort to understand the pyruvate branch point of this organism, fermentations under different inoculum and glucose concentrations were carried out. At low inoculum size (1x103 spores ml-1), high amount of lactate (78 g l-1) was produced, whereas high ethanol concentration (37 g l-1) was obtained at high inoculum sizes (1x106 spores ml-1). Decreasing working volume i...
Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes from the cephamycin C producer streptomyces clavuligerus
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The carbon flow through the lysine branch of aspartate pathway is a rate limiting step in the formation of cephamycin C, a broad spectrum P-lactam antibiotic produced by Streptomyces clavuligerus. In this study, the ask m(aspartokinase) and asd (aspartate semialdehyde dehydrogenase) genes which encode the enzymes catalyzing the first two steps of aspartate pathway were cloned and characterized as the first time from S. clavuligerus NRRL 3585. This was accomplished by constructing four mini-libraries of S. c...
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BibTeX
E. Alagoz, S. Acar, M. Yucel, and H. Hamamcı, “Cloning and screening of the putative hexokinase genes from Rhizopus oryzae and their heterologous expression in Saccharomyces cerevisiae,”
MOLECULAR BIOLOGY REPORTS
, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/99197.