Evaporation-Induced Biomolecule Detection on Versatile Superhydrophilic Patterned Surfaces: Glucose and DNA Assay

Download

index.pdf

Date

2018-10-01

Author

Beyazkılıç Ayas, Pınar
Saateh, Abtin
Bayindir, Mehmet
Elbuken, Caglar

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

77
views

66
downloads

We introduce a droplet-based biomolecular detection platform using robust, versatile, and low-cost superhydrophilic patterned superhydrophobic surfaces. Benefitting from confinement and evaporation-induced shrinkage of droplets on wetted patterns, we show enrichment-based biomolecular detection using very low sample volumes. First, we developed a glucose assay using fluorescent polydopamine (PDA) based on enhancement of PDA emission by hydrogen peroxide (H2O2) produced in enzyme-mediated glucose oxidation reaction. Incubation in evaporating droplets resulted in brighter fluorescence compared to that in bulk solutions. Droplet assay was highly sensitive toward increasing glucose concentration while that in milliliter-volume solutions resulted in no fluorescence enhancement at similar time scales. This is due to droplet evaporation that increased the reaction rate by causing enrichment of PDA and glucose/glucose oxidase as well as increased concentration of H2O2 generated in shrinking droplet. Second, we chemically functionalized wetted patterns with single-stranded DNA and developed fluorescence-based DNA detection to demonstrate the adaptability of the patterned surfaces for a different class of assay. We achieved detection of glucose and DNA with concentration down to 130 mu M and 200 fM, respectively. Patterned superhydrophobic surfaces with their simple production, sensitive response, and versatility present potential for bioanalysis from low sample volumes.

URI

https://hdl.handle.net/11511/96208

Journal

ACS OMEGA

DOI

https://doi.org/10.1021/acsomega.8b00389

Collections

Department of Chemical Engineering, Article

Suggestions

OpenMETU
Core

Hydrodynamic picture of light trapping in integrated photonic nanostructures and metamaterials Boriskina, Svetlana V.; Yerci, Selçuk; Chen, Gang (null; 2013-01-01) We introduce a concept of light trapping on the nanoscale via pinning optical vortices to optimally-designed nanostructures, reveal this mechanism behind known focusing phenomena, and offer new approaches to design nanophotonic platforms and/or coupling schemes. © 2013 Optical Society of America.
Cloaking Dynamics on Lubricant-Infused Surfaces Günay, Ahmet Alperen; Sett, Soumyadip; Ge, Qiaoyu; Zhang, Tiejun; Miljkovic, Nenad (2020-10-01) Lubricant-infused surfaces (SLIPSs/LISs) enable omniphobicity by reducing droplet pinning through creation of an atomically smooth liquid-liquid interface. Although SLIPSs/LISs provide efficient omniphobicity, the need for lubricant adds additional barriers to heat and mass transport and affects three-phase contact line dynamics. Here, evaporation dynamics of microscale water droplets on SLIPSs/LISs are investigated using steady and transient methods. Although steady results demonstrate that evaporation on ...
Investigation of tightly coupled arrays for wideband applications Arda, Kaan; Dural Ünver, Mevlüde Gülbin; Department of Electrical and Electronics Engineering (2020-10) This thesis aims to provide in depth research on tightly coupled dipole arrays to be used in ultrawideband apertures applications. First, operation principles of tightly coupled dipole arrays are investigated. Starting from the Wheeler’s current sheet aperture concept, some calculations on bandwidth and impedance concepts are conducted. B.A. Munk’s addition to the concept, use of capacitive elements between adjacent dipoles, are introduced. Array unit cell is modeled using equivalent circuit approach,...
Optimum topological design of geometrically nonlinear single layer lamella domes using harmony search method Çarbaş, Serdar; Saka, Mehmet Polat; Department of Engineering Sciences (2008) Harmony search method based optimum topology design algorithm is presented for single layer lamella domes. The harmony search method is a numerical optimization technique developed recently that imitates the musical performance process which takes place when a musician searches for a better state of harmony. Jazz improvisation seeks to find musically pleasing harmony similar to the optimum design process which seeks to find the optimum solution. The optimum design algorithm developed imposes the behavioral ...
Distributed adaptive output feedback control design and application to a formation flight experiment Kutay, Ali Türker; Calise, Anthony J.; D'Andrea, Raffaello (2005-01-01) An approach for augmenting existing distributed controller designs for large-scale interconnected systems with neural network based adaptive elements is proposed. It is assumed that the controllers are interconnected in the same way as the plant and based on the available measurements, a single hidden layer neural network is introduced for each subsystem to partially cancel the effects of the sub-system interconnections and modeling errors on tracking performance. Boundedness of error signals is shown throu...

Citation Formats

P. Beyazkılıç Ayas, A. Saateh, M. Bayindir, and C. Elbuken, “Evaporation-Induced Biomolecule Detection on Versatile Superhydrophilic Patterned Surfaces: Glucose and DNA Assay,” ACS OMEGA, vol. 3, no. 10, pp. 13503–13509, 2018, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/96208.