DEVELOPMENT OF A HYDROGEL BASED PROSTATE TUMOR MODEL FOR CANCER CELL MIGRATION STUDIES

Download

10447037.pdf

Date

2022-1-12

Author

Demirkaya, Damla

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

401
views

280
downloads

Prostate cancer is the second most diagnosed cancer in men and relapse due to the metastasis of cancer cells is the main reason of the high number of deaths in prostate cancer patients. Thus, there is a growing need for a tumor model to study the prostate cancer metastasis. In this study, a 3D model consisting of two natural polymer hydrogels (methacrylated collagen and methacrylated hyaluronic acid) with tunable mechanical and chemical properties carrying spheroids of two different prostate cancer cell lines (PC-3 and LNCAP) was developed to compare the effect of microenvironment on the migration. Fibroblast cells were incorporated in the hydrogel blend to increase the rate of migration of cancer cells. Migration of cancer cells in hydrogels with different compositions in the presence and absence of fibroblasts was studied with confocal laser scanning microscopy. The cancer cells in high collagen containing hydrogel were elongated, and the presence of fibroblasts in the microenvironment promoted the migration of prostate cancer cells by mimicking the microenvironment of prostate cancer more closely than other models. Epithelial-mesenchymal transition (EMT), a precondition for metastasis enhances mobility by decreasing epithelial markers and increasing mesenchymal markers. The quantification of migration was achieved through determination of E-cadherin and vimentin expression, the EMT marker proteins, through their fluorescence intensities in the immunostained samples. It was shown that E-cadherin expression was downregulated while vimentin expression was upregulated in the presence of fibroblast cells in high collagen containing hydrogel model as an indicator of migration.

Subject Keywords

Prostate cancer, Collagen, Migration, Hydrogel, Hyaluronic Acid

URI

https://hdl.handle.net/11511/96288

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Synthesis of novel indole-isoxazole hybrids and evaluation of their cytotoxic activities on hepatocellular carcinoma cell lines Hawash, Mohammed; Kahraman, Deniz Cansen; Ergun, Sezen Guntekin; Cetin-Atalay, Rengul; BAYTAŞ, SULTAN (2021-12-01) Background Liver cancer is predicted to be the sixth most diagnosed cancer globally and fourth leading cause of cancer deaths. In this study, a series of indole-3-isoxazole-5-carboxamide derivatives were designed, synthesized, and evaluated for their anticancer activities. The chemical structures of these of final compounds and intermediates were characterized by using IR, HRMS, H-1-NMR and C-13-NMR spectroscopy and element analysis. Results The cytotoxic activity was performed against Huh7, MCF7 and HCT116...
Synthesis of poly (dl-lactic-co-glycolic acid) coated magnetic nanoparticles for anti-cancer drug delivery Tansık, Gülistan; Gündüz, Ufuk; Department of Biology (2012) One of the main problems of current cancer chemotherapy is the lack of selectivity of anti-cancer drugs to tumor cells which leads to systemic toxicity and adverse side effects. In order to overcome these limitations, researches on controlled drug delivery systems have gained much attention. Nanoscale based drug delivery systems provide tumor targeting. Among many types of nanocarriers, superparamagnetic nanoparticles with their biocompatible polymer coatings can be targeted to an intented site by an extern...
Comparison of the Diagnostic Accuracy of Next Generation Sequencing and Microarray Resequencing Methods for Detection of BRCA1 and BRCA2 Gene Mutations Bahsi, Taha; Ergun, Sezen Güntekin; Ergun, Mehmet Ali; Perçin, E. Ferda (Gazi University, 2018-4-1) Objective: Breast cancer constitutes 29 % of estimated new cases of cancer in women, and it is also one of the major cause of death in all cancer types. In this study, DNA samples of familial breast cancer patients with BRCA1 and BRCA2 mutations which had been analyzed using conventional DNA sequencing method, were also analyzed with new methods including microarray and next generation sequencing (NGS) in order to compare their results Methods: Seven patients with BRCA1 mutation, one patient with BRCA2 m...
Investigation of the effects of combine use of cisplatin and metformin on proliferation of prostate cancer cell lines and gstp, cyp17a1 and hexokinase II enzymes Durukan, Özlem; Adalı, Orhan; Arslan, Şevki; Department of Biochemistry (2016) Prostate cancer is the most common cancer type both in developed and developing countries. Since 2015, it has been seen as one of the main cause of death in males, both in the world and in our country. Current therapeutic approaches for prostate cancer generally have variable efficiency, develop metastasis and drug resistance, and have high toxicity to normal tissues. Hence, the searching for more effective strategies with moderate or any adverse effects for the chemopreventive intervention of those cancers...
The Mechanism of anti tumorigenic effects of 15-lox-1 in colon cancer Çimen, İsmail; Banerjee, Sreeparna; Department of Biology (2012) Colorectal cancer is the 4th most widespread cause of cancer mortality. One of the pathways that are involved in the development of colorectal cancer is the arachidonic acid metabolizing lipoxygenase (LOX) pathway. Inflammatory molecules formed from this pathway exert profound effects that may exacerbate the development and progression of colon and other cancers. 15 lipoxygenase-1 (15-LOX-1) is a member of LOX protein family that metabolizes primarily linoleic acid to 13-(S)-HODE. Several lines of evidence ...

Citation Formats

D. Demirkaya, “DEVELOPMENT OF A HYDROGEL BASED PROSTATE TUMOR MODEL FOR CANCER CELL MIGRATION STUDIES,” M.S. - Master of Science, Middle East Technical University, 2022.