The effects of early postnatal ethanol intoxication on retina ganglion cell morphology and the development of retino-geniculate projections in mice

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2011
Dursun, İlknur
Experimental and clinical data have documented the adverse effects of perinatal ethanol intoxication on peripheral organs and the central nervous system. There is little known, however, about potential damaging effects of perinatal ethanol on the developing visual system. The purpose of this study was to examine the effects of neonatal ethanol intoxication on RGC morphology, estimate the total number of neurons in RGC layer and dorsolateral geniculate nucleus (dLGN), and on the eye-specific fiber segregation in the dLGN), in YFP and C57BL/6 mice pups. Ethanol (3 g/kg/day) was administered by intragastric intubation throughout postnatal days (PD) 3-20 or 3-10. Intubation control (IC) and untreated control (C) groups were included. Blood alcohol concentration (BAC) was measured in separate groups of pups on PD3, PD10, and PD20 at 4 different time points, 1, 1.5, 2 and 3 h after the second intubation. Numbers neurons in the RGCs and dLGN were quantified on PD10, PD20 using unbiased stereological procedures. The RGC images were taken using a confocal microscope and images were traced using Neurolucida software. On PD9, intraocular injections of cholera toxin-CTB) conjugated to Alexa 488 (green) or 594 (red) were administrated to the left and right eye, respectively. Measurements of the dLGN areas receiving projections from the v contralateral and ipsilateral eye were determined by using Image J (Image J, NIH). The early postnatal ethanol intoxication altered RGC morphology. Out of 13 morphological parameters examined in RGCs, soma area and dendritic field diameter were significantly reduced, while branch angles and dendrite tortuosity were increased. Additionally, a significant loss of RGCs and neurons in the dLGN was recorded at P20. The pixel intensity of total area and ipsilateral of retino-genuculate projection in the A group was significantly smaller as compared to the C group.

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Citation Formats
İ. Dursun, “The effects of early postnatal ethanol intoxication on retina ganglion cell morphology and the development of retino-geniculate projections in mice,” Ph.D. - Doctoral Program, Middle East Technical University, 2011.