The effects of early postnatal ethanol intoxication on retina ganglion cell morphology and the development of retino-geniculate projections in mice

Dursun, İlknur
Experimental and clinical data have documented the adverse effects of perinatal ethanol intoxication on peripheral organs and the central nervous system. There is little known, however, about potential damaging effects of perinatal ethanol on the developing visual system. The purpose of this study was to examine the effects of neonatal ethanol intoxication on RGC morphology, estimate the total number of neurons in RGC layer and dorsolateral geniculate nucleus (dLGN), and on the eye-specific fiber segregation in the dLGN), in YFP and C57BL/6 mice pups. Ethanol (3 g/kg/day) was administered by intragastric intubation throughout postnatal days (PD) 3-20 or 3-10. Intubation control (IC) and untreated control (C) groups were included. Blood alcohol concentration (BAC) was measured in separate groups of pups on PD3, PD10, and PD20 at 4 different time points, 1, 1.5, 2 and 3 h after the second intubation. Numbers neurons in the RGCs and dLGN were quantified on PD10, PD20 using unbiased stereological procedures. The RGC images were taken using a confocal microscope and images were traced using Neurolucida software. On PD9, intraocular injections of cholera toxin-CTB) conjugated to Alexa 488 (green) or 594 (red) were administrated to the left and right eye, respectively. Measurements of the dLGN areas receiving projections from the v contralateral and ipsilateral eye were determined by using Image J (Image J, NIH). The early postnatal ethanol intoxication altered RGC morphology. Out of 13 morphological parameters examined in RGCs, soma area and dendritic field diameter were significantly reduced, while branch angles and dendrite tortuosity were increased. Additionally, a significant loss of RGCs and neurons in the dLGN was recorded at P20. The pixel intensity of total area and ipsilateral of retino-genuculate projection in the A group was significantly smaller as compared to the C group.


Effects of Early Postnatal Exposure to Ethanol on Retinal Ganglion Cell Morphology and Numbers of Neurons in the Dorsolateral Geniculate in Mice
Dursun, Ilknur; Jakubowska-Dogru, Ewa; van der List, Deborah; Liets, Lauren C.; Coombs, Julie L.; Berman, Robert F. (Wiley, 2011-11-01)
Background: The adverse effects of fetal and early postnatal ethanol intoxication on peripheral organs and the central nervous system are well documented. Ocular defects have also been reported in about 90% of children with fetal alcohol syndrome, including microphthalmia, loss of neurons in the retinal ganglion cell (RGC) layer, optic nerve hypoplasia, and dysmyelination. However, little is known about perinatal ethanol effects on retinal cell morphology. Examination of the potential toxic effects of alcoh...
The Effects of Short-Term Chronic Ethanol Intoxication and Ethanol Withdrawal on the Molecular Composition of the Rat Hippocampus by FT-IR Spectroscopy
Elibol-Can, Birsen; Jakubowska-Dogru, Ewa; Severcan, Mete; Severcan, Feride (Wiley, 2011-11-01)
Background: The numerous adverse effects of ethanol abuse and ethanol withdrawal on biological systems are well documented. Conversely, the understanding of the molecular mechanisms underlying these pathological effects is still incomplete. This study was undertaken to investigate the effects of short-term chronic ethanol administration and ethanol withdrawal on the molecular structure and function of hippocampal tissue, a brain region important for mnemonic processes and known to be highly susceptible to e...
The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum
Kocabıyık, Semra; Russel, RJM; Danson, MJ; Hough, DW (Elsevier BV, 1996-07-05)
In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels and have investigated the effects of this mutation on the biochemical properties and thermal stability of the enzyme. Both wild-type and the mutant enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The mutant Thermoplasma citrate synthase is very similar to wild-type citrate s...
The expression of GST isoenzymes and p53 in non-small cell lung cancer.
Oguztßzun, Serpil; Aydin, Mehtap; Demirag, Funda; Yazici, Ulkß; Ozhavzali, Mßzeyyen; Kiliç, Murat; Can, Mesude IŠ(VM Media SP. zo.o VM Group SK, 2010-6-10)
This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase alpha, pi, mu, theta and p53 in non-small cell lung carcinoma and normal lung tissue from 50 patients. The relationships between expressions of the Glutathione-S-transferase isoenzymes and some clinicopathological features were also examined. Expression of glutathione-S-transferase pi, mu, alpha, theta and p53 was assessed by immunohistochemistry for primary lung carcinomas of 50 patients from the Sanitariu...
The structural effects of Vitamin A deficiency on biological macromolecules due to ethanol consumption and withdrawal: An FTIR study with chemometrics
ELİBOL, BİRSEN; Severcan, Mete; Jakubowska-Dogru, Ewa; DURSUN, İLKNUR; Severcan, Feride (2022-04-01)
The structural effects of vitamin A-deficiency were examined on the molecular profiles of biomolecules of male rat hippocampus during prolonged ethanol intake/withdrawal using FT-IR spectroscopy coupled with chemometrics. Liquid ethanol diet with/without vitamin A was maintained to adult rats for 3-months. The rats were decapitated at different ethanol withdrawal times and FT-IR spectra were obtained. Ethanol consumption/withdrawal produced significant changes in proteins' conformations, while having insign...
Citation Formats
İ. Dursun, “The effects of early postnatal ethanol intoxication on retina ganglion cell morphology and the development of retino-geniculate projections in mice,” Ph.D. - Doctoral Program, Middle East Technical University, 2011.