Clinical validation of SERS metasurface SARS-CoV-2 biosensor

Date

2022-01-01

Author

Bilgin, Buse
Torun, Hülya
İlgü, Müslüm
Yanik, Cenk
Batur, Sükrü Numan
Çelik, Süleyman
Öztürk, Meriç
Dogan, Özlem
Ergönül, Önder
Solaroglu, Ihsan
Can, Füsun
Onbasli, Mehmet Cengiz

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

129
views

0
downloads

© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.The real-Time polymerase chain reaction (RT-PCR) analysis using nasal swab samples is the gold standard approach for COVID-19 diagnosis. However, due to the high false-negative rate at lower viral loads and complex test procedure, PCR is not suitable for fast mass screening. Therefore, the need for a highly sensitive and rapid detection system based on easily collected fluids such as saliva during the pandemic has emerged. In this study, we present a surface-enhanced Raman spectroscopy (SERS) metasurface optimized with genetic algorithm (GA) to detect SARS-CoV-2 directly using unprocessed saliva samples. During the GA optimization, the electromagnetic field profiles were used to calculate the field enhancement of each structure and the fitness values to determine the performance of the generated substrates. The obtained design was fabricated using electron beam lithography, and the simulation results were compared with the test results using methylene blue fluorescence dye. After the performance of the system was validated, the SERS substrate was tested with inactivated SARS-CoV-2 virus for virus detection, viral load analysis, cross-reactivity, and variant detection using machine learning models. After the inactivated virus tests are completed, with 36 PCR positive and 33 negative clinical samples, we were able to detect the SARS-CoV-2 positive samples from Raman spectra with 95.2% sensitivity and specificity.

Subject Keywords

COVID-19, Genetic algorithm, Metasurface, Oligonucleotide, Plasmonics, Raman, Surface-enhanced raman spectroscopy

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85129808487&origin=inward
https://hdl.handle.net/11511/97617

DOI

https://doi.org/10.1117/12.2607929

Conference Name

Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry

Collections

Department of Biology, Conference / Seminar

Suggestions

OpenMETU
Core

New neutral gauge bosons in supersymmetric U(1)0 models at hadron colliders Frank, Mariana; SELBUZ, LEVENT; Turan, İsmail (2021-01-01) © Copyright owned by the author(s) under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0)We examine the production and decay modes of Z0 boson at hadron colliders in an U(1)0-extended MSSM. We choose a U(1)0 model with a secluded sector, where the tension between the electroweak scale and developing a large enough mass for Z0 is resolved by incorporating three additional SU(2) singlet fields into the model. We perform a detailed and system...
Numerical heat transfer modelling of a centrifugal solar particle receiver Hiçdurmaz, Serdar; Johnson, Evan F.; Grobbel, Johannes; Amsbeck, Lars; Buck, Reiner; Hoffschmidt, Bernhard (2022-05-12) © 2022 Author(s).Solar particle receivers promise higher operating temperatures compared to molten salt-based receivers. The Discrete Element Method (DEM) tool LIGGGHTS is used to simulate the particle motion in a centrifugal solar particle receiver. A separate thermal model is developed in MATLAB, which uses the particle positions calculated with the DEM simulation. Heat transfer at the particle scale is accounted for with models of particle-particle conduction, particle-fluid- particle conduction, and a n...
Energy preserving reduced-order modeling of the rotating thermal shallow water equation Karasözen, Bülent; Ylldlz, S.; Uzunca, M. (2022-05-01) © 2022 Author(s).In this paper, reduced-order models (ROMs) are developed for the rotating thermal shallow water equation (RTSWE) in the non-canonical Hamiltonian form with state-dependent Poisson matrix. The high fidelity full solutions are obtained by discretizing the RTSWE in space with skew-symmetric finite-differences, while preserving the Hamiltonian structure. The resulting skew-gradient system is integrated in time by the energy preserving average vector field (AVF) method. The ROM is constructed by...
Linear and nonlinear optical characteristics of PbMoO4 single crystal for optoelectronic applications Delice, S.; Isik, M.; Hasanlı, Nızamı (2022-10-01) © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.In the present paper, we studied linear and nonlinear optical properties of lead molybdate (PbMoO4) single crystals grown by Czochralski method. Raman measurement was performed in the range of 50–1000 cm−1 and 11 active vibration modes were defined in the spectrum. The nature of the observed modes was discussed in detail. Optical absorption of the material was investigated utilizing room temperatu...
Simulation model fidelity enhancement using corrective force and moment increments: Review of activity performed in NATO-AVT Panel 296 Taghizad, Armin; White, Mark; Cameron, Neil; Padfield, Gareth; Yavrucuk, İlkay; Myrand-Lapierre, Vincent; Nadeau-Beaulieu, Michel; Richard, Sylvain; Tischler, Mark (2021-01-01) Copyright © 2021 by the Vertical Flight Society. All rights reserved.The Applied Vehicle Technology (AVT) Panel of the NATO Science and Technology Organization has recently engaged a Research Task Group on the topic of rotorcraft flight simulation model fidelity. This group aimed to explore a comprehensive set of methods for flight mechanics simulation fidelity enhancement, including training simulation applications. Particular effort was also directed to the metrics used for simulation fidelity model asses...

Citation Formats

B. Bilgin et al., “Clinical validation of SERS metasurface SARS-CoV-2 biosensor,” Virtual, Online, 2022, vol. 11957, Accessed: 00, 2022. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85129808487&origin=inward.