Development of a microfluidic platform to maintain viability of micro-dissected tumor slices in culture

Date

2022-05-01

Author

Parsian, Maryam
MUTLU, PELİN
Yıldırım, Ender
Ildiz, Can
Özen, Can
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

164
views

0
downloads

© 2022 Author(s).One of the issues limiting the development of personalized medicine is the absence of realistic models that reflect the nature and complexity of tumor tissues. We described a new tissue culture approach that combines a microfluidic chip with the microdissected breast cancer tumor. "Tumor-on-a-chip"devices are suitable for precision medicine since the viability of tissue samples is maintained during the culture period by continuously feeding fresh media and eliminating metabolic wastes from the tissue. However, the mass transport of oxygen, which arguably is the most critical nutrient, is rarely assessed. According to our results, transportation of oxygen provides satisfactory in vivo oxygenation within the system. A high level of dissolved oxygen, around 98%-100% for every 24 h, was measurable in the outlet medium. The microfluidic chip system developed within the scope of this study allows living and testing tumor tissues under laboratory conditions. In this study, tumors were generated in CD-1 mice using MDA-MB-231 and SKBR-3 cell lines. Microdissected tumor tissues were cultured both in the newly developed microfluidic chip system and in conventional 24-well culture plates. Two systems were compared for two different types of tumors. The confocal microscopy analyses, lactate dehydrogenase release, and glucose consumption values showed that the tissues in the microfluidic system remained more viable with respect to the conventional well plate culturing method, up to 96 h. The new culturing technique described here may be superior to conventional culturing techniques for developing new treatment strategies, such as testing chemotherapeutics on tumor samples from individual patients.

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85129820620&origin=inward
https://hdl.handle.net/11511/97622

Journal

Biomicrofluidics

DOI

https://doi.org/10.1063/5.0087532

Collections

Department of Mechanical Engineering, Article

Suggestions

OpenMETU
Core

Development of a three-dimensional basin model to evaluate the site effects in the tectonically active near-fault region of Gölyaka basin, Düzce, Turkey Yousefi-Bavil, Karim; KOÇKAR, MUSTAFA KEREM; Akgün, Haluk (2022-01-01) © 2022, The Author(s), under exclusive licence to Springer Nature B.V.The high seismicity and tectonic activity of the study area located in a near-fault region in Gölyaka, Düzce, results in a bedrock geometry highly complex in the sense of faulting and deformation. This makes this area very challenging in terms of a site response study that would aid seismic hazard assessment. This study develops a basin model to evaluate the site effects in the tectonically formed Plio-Quaternary fluvial sedimentary layer...
Numerical heat transfer modelling of a centrifugal solar particle receiver Hiçdurmaz, Serdar; Johnson, Evan F.; Grobbel, Johannes; Amsbeck, Lars; Buck, Reiner; Hoffschmidt, Bernhard (2022-05-12) © 2022 Author(s).Solar particle receivers promise higher operating temperatures compared to molten salt-based receivers. The Discrete Element Method (DEM) tool LIGGGHTS is used to simulate the particle motion in a centrifugal solar particle receiver. A separate thermal model is developed in MATLAB, which uses the particle positions calculated with the DEM simulation. Heat transfer at the particle scale is accounted for with models of particle-particle conduction, particle-fluid- particle conduction, and a n...
Use of Auxetic Infill Structures for the Compensation of Shrinkage in Fused Filament Fabrication Process Uncu, Rahman; Bacikoglu, Mehmet Canberk; Caliskan, Burak; Yaman, Ulaş (2023-01-01) The polymer parts fabricated by Fused Filament Fabrication (FFF) process deteriorated noticeably in terms of dimensional accuracy due to the thermal processes during the fabrication and cooling phases. Commonly, researchers tend to concentrate on modifying the CAD model or fabricating the parts larger than the nominal then post-processing the artifacts to compensate for shrinkage. This paper investigates the effects of auxetic infill structures on shrinkage compensation. Parts with different internal geomet...
Modeling coastal Güzelyurt (Morphou) aquifer in northern Cyprus for mitigation of groundwater depletion through managed aquifer recharge Demir, Cansu; Fanta, Debebe; Akıntuğ, Bertuğ; Ünlü, Kahraman (2022-08-01) © 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.Groundwater depletion is considered a global problem. Coastal aquifers in arid and semiarid areas of the Mediterranean basin constitute typical examples of depleted aquifers. Güzelyurt (Morphou) Aquifer, the most important water resource for northern Cyprus, faces the problems of groundwater depletion and degradation of water quality near the coast due to significant amounts of groundwater extraction to satisfy the high demand....
Determination of the spectroscopic parameters of beauty-partners of Tcc from QCD Alıyev, Tahmasıb; Savcı, Mustafa; Bilmiş, Selçuk (2022-04-01) © 2022 authors. Published by the American Physical Society.Motivated by the recent discovery of a new tetraquark Tcc+ state with two charm quarks and two light quarks by LHCb Collaboration, we calculate the spectroscopic parameters; namely, the mass and residues of beauty partners of Tcc+ within QCD sum rules. The obtained results are compared with the predictions of different approaches in the literature.

Citation Formats

M. Parsian, P. MUTLU, E. Yıldırım, C. Ildiz, C. Özen, and U. Gündüz, “Development of a microfluidic platform to maintain viability of micro-dissected tumor slices in culture,” Biomicrofluidics, vol. 16, no. 3, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85129820620&origin=inward.