Investigation of plasmid mediated colistin resistance phenotypically and genotypically in Salmonella and E. coli isolates in Turkey

Download

TEZ-SERAY TOK.pdf

Date

2022-5-31

Author

Tok, Seray

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

315
views

137
downloads

Today, colistin is used as the last resort antimicrobial against Gram-negative bacteria. Excessive use of colistin caused resistance among bacteria. Recent studies showed presence of plasmid mediated mcr genes, encoding colistin resistance that can be transferred horizontally between different bacterial species. In this study, it was aimed to investigate colistin resistance and change in colistin resistance rate in E. coli and Salmonella isolates over time from different cities in Turkey. For this aim, we used 2 groups of isolates as recent (collected in 2018-2019) and historical (collected in 2010-2015). E. coli and Salmonella isolates, were isolated from 238 chicken meat samples collected between 2018-2019 in Ankara in this study. 36 E. coli and 16 Salmonella were isolated. All strains were tested for antimicrobial resistance for colistin and 18 antimicrobial agents other than colistin. 197 Salmonella isolates, representing historical isolates collected between 2010-2015 from human, animal and food samples and 48 E. coli isolates collected in 2011-2015 from food samples were investigated for colistin resistance. Phenotypic resistance was observed using disc diffusion and MIC test. Genotypic resistance characterization was investigated using PCR. 25 % and 53 % of recent, 0 % and %8 of historical E. coli and Salmonella isolates showed evidence of colistin resistance. Colistin resistance was seen to be increased over time among both Salmonella and E. coli isolates. We investigated that colistin resistance in isolates used in this study was not due to plasmid mediated genes, but most likely due to the chromosomal mutations. This assumption should be further investigated.

Subject Keywords

Antimicrobial resistance, E. coli, Salmonella

URI

https://hdl.handle.net/11511/98106

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Determination of colistin resistance in Escherichia coli isolates from foods in Turkey, 2011-2015 Güzel, Mustafa; Avşaroğlu, M Dilek; Soyer, Yeşim (2020-05-01) Antimicrobial resistance of pathogenic microorganisms is an emerging public health concern. Intensive use of antibiotics in food animals might increase antimicrobial resistance in foodborne pathogens. Colistin is a last resort antibiotic for treatment of multidrug resistant (MDR) Gram negative pathogens. The recent antimicrobial resistance studies revealed a mobile antimicrobial resistance gene (mcr) that provides resistance to colistin. Furthermore, the gene has been found in different genera. Therefore, t...
Study on Cost-Efficient Carbon Aerogel to Remove Antibiotics from Water Resources Aylaz, Gülgün; Okan, Meltem; Duman, Memed; Aydin, Halil Murat (American Chemical Society (ACS), 2020-6-28) Because of pharmaceutical-emerging contaminants in water resources, there has been a significant increase in the antibiotic resistance in bacteria. Therefore, the removal of antibiotics from water resources is essential. Various antibiotics have been greatly studied using many different carbon-based materials including graphene-based hydrogels and aerogels. In this study, carbon aerogels (CAs) were synthesized from waste paper sources and their adsorption behaviors toward three antibiotics (hygromycin B, ge...
Discovery of non-carbohydrate inhibitors of aminoglycoside-modifying enzymes Welch, KT; Virga, KG; Whittemore, NA; Özen, Can; Wright, E; Brown, CL; Lee, RE; Serpersu, EH (2005-11-15) Chemical modification and inactivation of aminoglycosides by many different enzymes expressed in pathogenic bacteria are the main mechanisms of bacterial resistance to these antibiotics. In this work, we designed inhibitors that contain the 1,3-diamine pharmacophore shared by all aminoglycoside antibiotics that contain the 2-deoxystreptamine ring. A discovery library of molecules was prepared by attaching different side chains to both sides of the 1,3-diamine motif. Several of these diamines showed inhibito...
Investigation of the Ligand Binding Characteristics of Staphylococcus aureus NorA Efflux Pump Işık , Esra Büşra; Serçinoğlu, Onur (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Staphylococcus aureus is a gram-positive bacterial pathogen which is highly adaptive to environmental conditions and causes various disorders. Excessive usage of antibiotics may result in development of antibiotic resistance in S. aureus. One of the resistance mechanisms is increase in the activity of transmembrane multi-drug efflux pumps. NorA is the most studied efflux pump in S. aureus, which belongs to Major Facilitator Superfamily (MFS). NorA has been shown to contribute to resistance against a variety...
Reconstruction of the temporal signaling network in Salmonella-infected human cells Budak, Gungor; Ozsoy, Oyku Eren; Aydın Son, Yeşim; Can, Tolga; Tunçbağ, Nurcan (2015-07-20) Salmonella enterica is a bacterial pathogen that usually infects its host through food sources. Translocation of the pathogen proteins into the host cells leads to changes in the signaling mechanism either by activating or inhibiting the host proteins. Given that the bacterial infection modifies the response network of the host, a more coherent view of the underlying biological processes and the signaling networks can be obtained by using a network modeling approach based on the reverse engineering principl...

Citation Formats

S. Tok, “Investigation of plasmid mediated colistin resistance phenotypically and genotypically in Salmonella and E. coli isolates in Turkey,” M.S. - Master of Science, Middle East Technical University, 2022.