Analyzing the expression patterns of vitamin D metabolizing CYP27B1 and CYP24A1 in brain tissue of vitamin D treated mice with Multiple Sclerosis (MS)

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2022-8

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Özdoğan, Dilara

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The etiopathogenesis of Multiple Sclerosis (MS), an inflammatory demyelinating autoimmune disease of the central nervous system, is still unknown. MS is a complex, recurring, and frequently progressing condition. There is a hypothesis that MS is adversely associated with the length and intensity of sunlight exposure and vitamin D concentrations since MS frequency rises with increasing latitude. A female C57BL/6 mouse model for autoimmune encephalomyelitis (EAE) was used in this investigation to examine the associations between vitamin D supplementation, MS, and the vitamin D metabolizing CYP enzymes CYP27B1, and CYP24A1 at the protein expression levels. Initially, mice were separated into four groups: Gr 1 (Control), Gr 2 (Vitamin D supplemented control), Gr 3 (EAE – MS Model), and Gr 4 (vitamin D supplemented EAE – MS Model). The protein expression levels of Gr 1 were adjusted to 1.00 fold, and expression levels of the other groups were determined relative to Gr 1. No statistically significant difference was observed in mice brain CYP27B1 and CYP24A1 protein expressions among the groups. However, there was a slight decrease in the mice brain CYP24A1 protein expressions of vitamin D supplemented groups (Gr 2 and Gr 4) compared to their control groups (Gr 1 and Gr 3). In conclusion, in the future both CYP24A1 inhibitor therapy and vitamin D supplementation might be tested out to assist reducing the intensity of MS symptoms.

Subject Keywords

Vitamin D, Multiple Sclerosis (MS), Vitamin D Metabolizing CYPs, Experimental Autoimmune Encephalomyelitis (EAE), Brain

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https://hdl.handle.net/11511/98612

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

D. Özdoğan, “Analyzing the expression patterns of vitamin D metabolizing CYP27B1 and CYP24A1 in brain tissue of vitamin D treated mice with Multiple Sclerosis (MS),” M.S. - Master of Science, Middle East Technical University, 2022.