Investigation of Glutathione S-Transferase (GST) expression and activity in mouse with Multiple Sclerosis (MS)

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DENİZ ARÇAK-MSc thesis.pdf

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2022-5-11

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Arçak, Deniz

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Multiple Sclerosis (MS) of unknown etiopathogenesis is a chronic demyelinating disease of the central nervous system. It mainly destroys myelin in the brain and spinal cord. Non-traumatic injuries have been observed in this disease for young adults. Various factors affect MS, but oxidative stress is one of the most important causes of demyelination. Glutathione S- Transferases (GSTs) can be described as a versatile enzyme family of eukaryotic and prokaryotic phase II metabolic isoenzymes. They have enzymatic or non-enzymatic functions in the body. Their main task is to conjugate GSH to endogenous and exogenous electrophilic compounds for the detoxification process. In this study, it was aimed to reveal the relationship between the activity and protein expression of the Glutathione S-Transferase (GST) enzyme family and MS disease in a female C57BL/6 mouse autoimmune encephalomyelitis (EAE) model. There was no statistically significant difference (p ≤ 0.05) in mouse liver GST protein expression between the two groups of animals, the MS patient model and the control group. However, higher GST enzyme activity was detected in the MS group compared to the control group. In conclusion, considering the post-translational modifications affecting GST members in some pathways, this study could lead to the development of a new drug metabolized by GST that can be used in the treatment of MS and studied in detail in the future.

Subject Keywords

Multiple Sclerosis, Glutathione S-Transferase, Experimental Autoimmune Encephalomyelitis, Mouse

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https://hdl.handle.net/11511/97331

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

D. Arçak, “Investigation of Glutathione S-Transferase (GST) expression and activity in mouse with Multiple Sclerosis (MS),” M.S. - Master of Science, Middle East Technical University, 2022.