Investigation of Glutathione S-Transferase (GST) expression and activity in mouse with Multiple Sclerosis (MS)

2022-5-11
Arçak, Deniz
Multiple Sclerosis (MS) of unknown etiopathogenesis is a chronic demyelinating disease of the central nervous system. It mainly destroys myelin in the brain and spinal cord. Non-traumatic injuries have been observed in this disease for young adults. Various factors affect MS, but oxidative stress is one of the most important causes of demyelination. Glutathione S- Transferases (GSTs) can be described as a versatile enzyme family of eukaryotic and prokaryotic phase II metabolic isoenzymes. They have enzymatic or non-enzymatic functions in the body. Their main task is to conjugate GSH to endogenous and exogenous electrophilic compounds for the detoxification process. In this study, it was aimed to reveal the relationship between the activity and protein expression of the Glutathione S-Transferase (GST) enzyme family and MS disease in a female C57BL/6 mouse autoimmune encephalomyelitis (EAE) model. There was no statistically significant difference (p ≤ 0.05) in mouse liver GST protein expression between the two groups of animals, the MS patient model and the control group. However, higher GST enzyme activity was detected in the MS group compared to the control group. In conclusion, considering the post-translational modifications affecting GST members in some pathways, this study could lead to the development of a new drug metabolized by GST that can be used in the treatment of MS and studied in detail in the future.

Suggestions

INVESTIGATION OF EFFECTS OF VITAMIN D AND VITAMIN D METABOLIZING CYP450 ISOZYMES ON MULTIPLE SCLEROSIS PATHOPHYSIOLOGY IN ANIMAL MODEL BY MOLECULAR APPROACHES
Evin, Emre; Adalı, Orhan; Department of Biology (2021-8)
Multiple sclerosis (MS) is a complicated, recurrent, and often progressive inflammatory demyelinating autoimmune disease of the central nervous system, yet etiopathogenesis remains unsolved. MS frequency increases with increasing latitude, leading to a hypothesis that MS is inversely correlated with the duration and intensity of sunlight and vitamin D concentrations. In this study, the relationships between vitamin D supplementation, MS, VDR, and vitamin D metabolizing CYP enzymes, including CYP2R1, CYP27A1...
Analyzing the expression patterns of vitamin D metabolizing CYP27B1 and CYP24A1 in brain tissue of vitamin D treated mice with Multiple Sclerosis (MS)
Özdoğan, Dilara; Adalı, Orhan; Evin, Emre; Department of Molecular Biology and Genetics (2022-8)
The etiopathogenesis of Multiple Sclerosis (MS), an inflammatory demyelinating autoimmune disease of the central nervous system, is still unknown. MS is a complex, recurring, and frequently progressing condition. There is a hypothesis that MS is adversely associated with the length and intensity of sunlight exposure and vitamin D concentrations since MS frequency rises with increasing latitude. A female C57BL/6 mouse model for autoimmune encephalomyelitis (EAE) was used in this investigation to examine the ...
INVESTIGATION OF INFLAMMATION ASSOCIATED CYP2E1 AND CYP1A1 EXPRESSION IN THE EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS MOUSE MODEL OF MULTIPLE SCLEROSIS
Solak, Damla; Adalı, Orhan; Department of Molecular Biology and Genetics (2023-1-27)
Multiple sclerosis (MS) is an autoimmune neurodegenerative disease which affects brain and spinal cord. Myelin which wraps around the axon and its progenitor cells, oligodendrocytes, are destroyed in this disease. This results in the loss of signal transmission which leads to axonal, and eventually neuronal loss. There are various symptoms of the disease such as unstable feelings, fatigue, visual disability, muscle spasms, and walking difficulties. The etiology of the disease is still in its infancy; howeve...
Generation and Characterization of Induced Pluripotent Stem Cell Lines From Multiple Sclerosis Patients and Healthy Individuals
Begentaş, Onur Can; Kiriş, Erkan; Department of Molecular Biology and Genetics (2021-9-8)
Multiple Sclerosis (MS) is an autoimmune disease characterized by inflammation, demyelination, and axonal damage resulting in neurodegeneration in the central nervous system (CNS). Relapsing-remitting MS (RRMS) is the most common form of the disease known to affect more than 2 million individuals globally, and the prevalence of the disease is increasing worldwide. As a chronic condition without a cure, RRMS manifests in a relapsing-remitting form with sporadic attacks suddenly appearing, causing neurologica...
Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis
Koc, Dilara; Begentaş, Onur Can; Yurtogullari, Sukran; Temel, Musa; Akcali, Kamil Can; Demirkaya, Seref; Kiriş, Erkan (2021-05-01)
Multiple sclerosis is a chronic disease characterized by inflammation, demyelination, and axonal damage in the central nervous system. Here, we established an induced pluripotent stem cell (iPSC) line METUi001-A from the peripheral blood mononuclear cells of a 25-year-old male individual with clinically diagnosed Relapsing-Remitting Multiple Sclerosis (RRMS) using the integration-free Sendai reprogramming method. We demonstrated that the iPSCs are free of exogenous Sendai reprogramming vectors, have a norma...
Citation Formats
D. Arçak, “Investigation of Glutathione S-Transferase (GST) expression and activity in mouse with Multiple Sclerosis (MS),” M.S. - Master of Science, Middle East Technical University, 2022.