CELL CYCLE-DEPENDENT REGULATION OF CXXC5 SYNTHESIS

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Thesis Final_Öykü Deniz Demiralay.pdf

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2022-8-22

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Demiralay, Öykü Deniz

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17β-estradiol (E2) is the main estrogen in circulation and has many physiological effects on various tissues, including the mammary tissue. CXXC5 is an estrogen-responsive gene product that binds to nonmethylated CpG dinucleotides on DNA. CXXC5 synthesis shows fluctuation in the cell cycle. This led to our prediction that the level of CXXC5 synthesis is regulated through the cell cycle. To test this prediction, I investigated the synthesis of CXXC5 in cell cycle-synchronized cells for every 6h up to 36h. I found that the level of CXXC5 synthesis shows alterations with the cell cycle. To investigate cell cycle-dependent expression and/or synthesis of CXXC5, I in this thesis established experimental conditions and protocols. These findings lay the foundations for future studies that aim to further delve into mechanisms of CXXC5 expression, synthesis, and degradation in a cell cycle-dependent manner.

Subject Keywords

Estrogen, Estrogen Receptor, CXXC5, Cell Cycle

URI

https://hdl.handle.net/11511/98645

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

Ö. D. Demiralay, “CELL CYCLE-DEPENDENT REGULATION OF CXXC5 SYNTHESIS,” M.S. - Master of Science, Middle East Technical University, 2022.