Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae

2016-05-01
Uyar, Ebru Ozer
Yucel, Meral
Hamamcı, Haluk
Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.
Citation Formats
E. O. Uyar, M. Yucel, and H. Hamamcı, “Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae,” pp. 459–468, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30413.