Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae

Date

2016-05-01

Author

Uyar, Ebru Ozer
Yucel, Meral
Hamamcı, Haluk

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Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.

Subject Keywords

Cloning, Rhizopus oryzae, Trehalose, Trehalose-6-phosphate synthase, Trehalose 6-phosphate synthase, Saccharomyces-cerevisiae, Selaginella-lepidophylla, Molecular characterization, Reserve carbohydrate, Escherichia-coli, Yeast, Gene, Stress, Complementation

URI

https://hdl.handle.net/11511/30413

Journal

JOURNAL OF BASIC MICROBIOLOGY

DOI

https://doi.org/10.1002/jobm.201500425

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

E. O. Uyar, M. Yucel, and H. Hamamcı, “Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae,” JOURNAL OF BASIC MICROBIOLOGY, pp. 459–468, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30413.