Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Optimization of Ethanol Production From Microfluidized Wheat Straw by Response Surface Methodology
Date
2015-11-17
Author
Turhan, Ozge
Isci, Asli
Mert, Behiç
Sakiyan, Ozge
Donmez, Sedat
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
275
views
0
downloads
Cite This
In this study, wheat straw was pretreated with a microfluidizer to improve its enzymatic hydrolysis and ethanol yields. The pretreatment was performed at various pressures (500, 1000, and 1500bar) and solid loadings (1, 2, and 3%). The microfluidized biomass was then subjected to hydrolysis and simultaneous saccharification and co-fermentation (SSCF) experiments at different enzyme loadings (5, 10, and 15 FPU/g dry wheat straw) using a mutant yeast. The results indicated that the microfluidization method alters the structure of biomass and leads to a reduction in lignin content. The samples pretreated at 1% solid loading contained the minimum lignin concentration and provided the maximum sugar and ethanol yields. These results signified that the microfluidization method is more effective on biomass at low solid loadings. The process conditions were optimized for higher ethanol and sugar yields using response surface methodology (RSM). The optimum pressure and solid and enzyme loadings were found as 1500bar, 1%, and 15 FPU/g dry wheat straw, respectively. The yields obtained at this condition were 82%, 94%, and 65% for glucose, xylose, and ethanol, respectively. High sugar yields implied that microfluidization is an effective pretreatment method for cellulosic ethanol production. On the other hand, low ethanol yield may indicate that the microorganism was sensitive to inhibitory compounds present in the fermentation medium.
Subject Keywords
Wheat straw
,
Pretreatment
,
Microfluidization
,
Bioethanol
,
Enzymatic hydrolysis
,
Lignocellulosic biomass
URI
https://hdl.handle.net/11511/35761
Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
DOI
https://doi.org/10.1080/10826068.2014.958164
Collections
Department of Food Engineering, Article
Suggestions
OpenMETU
Core
Production of thermophylic α-amylase from aspergillus sp. and its utilization for various applications
Gazaloğlu, Mehmet; Hamamcı, Haluk; Department of Biotechnology (2017)
The objective of this study was to scale up the production of thermophilic α-amylase and its use to obtain fermentable sugar as the main carbon source for microorganisms such as baker’s yeast and Lactobacillus casei. Aspergillus niger N402 was the strain used for determining the fermentation parameters (carbon and nitrogen source, fermentation volume/total flask volume ratio (FV/TFV), pH & temperature of the fermentation and spore inoculum level.) Different strains (Am13, AmC18, and AmC28) were also tested ...
Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-10-01)
© 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimizat...
Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01)
Lignocellulolytic enzymes such as cellulases and xylanases are needed on the industrial scales for low cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focuses on the statistical optimization of fungal inoc...
Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (Elsevier BV, 2019-06-01)
Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreat...
Enhancement of storage stability of wheat germ oil by encapsulation
KARADENIZ, Meltem; Şahin, Serpil; Şümnü, Servet Gülüm (2018-04-01)
Wheat germ oil which is a rich source of a-tocopherol is susceptible to oxidation. The main objective of this study was to encapsulate wheat germ oil to enhance its oxidation stability. It was also aimed to investigate the effects of different homogenization methods on physicochemical properties and storage stability of encapsulated wheat germ oil. As homogenization methods, silent crusher (SC), microfluidization (MF) and ultrasonication (US) were used. SC and MF techniques created more stable emulsions tha...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
O. Turhan, A. Isci, B. Mert, O. Sakiyan, and S. Donmez, “Optimization of Ethanol Production From Microfluidized Wheat Straw by Response Surface Methodology,”
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
, pp. 785–795, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35761.