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Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Date
2022-01-01
Author
Iram, Attia
Çekmecelioğlu, Deniz
Demirci, Ali
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Lignocellulolytic enzymes such as cellulases and xylanases are needed on the industrial scales for low cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focuses on the statistical optimization of fungal inoculum size (1-10%), aeration (0.5-2vvm) and agitation rates (100-500rpm) through response surface methodology (RSM) to obtain highest amounts of cellulases and xylanases. Aspergillus niger (NRRL 330) was grown in dilute acid treated DDGS supplemented with peptone, yeast extract, and ammonium sulfate in benchtop bioreactors to produce these enzymes. The optimal fermentation results were determined to be 6.5% inoculum size, 310 rpm agitation rate and 1.4 vvm aeration, which revealed that cellulase activity was increased from 0.6 to 0.82 IU/ml and xylanase activity increased from 3.99 IU/ml to 52.76 IU/ml after optimization. The results prove the positive effect of optimal culture conditions such as aeration, agitation and inoculum size on the production of hydrolytic enzymes by A. niger using DDGS as the main feedstock.
Subject Keywords
Aspergillus niger
,
cellulase
,
DDGS
,
Hydrolytic enzymes
,
lignocellulosic biomass
,
xylanase
URI
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85137569855&origin=inward
https://hdl.handle.net/11511/101479
DOI
https://doi.org/10.13031/aim.202200146
Conference Name
2022 ASABE Annual International Meeting
Collections
Department of Food Engineering, Conference / Seminar
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Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-10-01)
© 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimizat...
The effect of Dilution, Aeration, and Agitation on Fungal Cellulase and Xylanase Production by DDGS-based Fermentation Media in Stirred Tank Bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01)
Fungal cellulases and xylanases are commonly used for biological pretreatment of lignocellulosic biomass into simple sugars, which can then be converted into many value-added products including bioethanol. However, these enzymes are costly to produce and need a feedstock that should be more economical and high in fiber and other nutrients. Distillers dried grains with solubles (DDGS) is the byproduct of corn ethanol production and can be considered an appropriate feedstock. Among various problems in adaptat...
Improvement of Pectinase Production by Co-culture of Bacillus spp. Using Apple Pomace as a Carbon Source
Kuvvet, Ceren; UZUNER, SİBEL; Çekmecelioğlu, Deniz (2019-05-01)
Increasing interest in using food and agricultural wastes for low cost enzyme production continues in fermentation industry. Bacteria, especially Bacillus subtilis, are known to produce pectinases in synthetic media and media amended with agricultural wastes as a carbon source, whereas the potential of co-culturing Bacillus species to produce pectinases has not been fully studied yet. In this study, co-culture of B. subtilis and B. pumilus was optimized for solid load and pH to maximize pectinase production...
Optimization of Ethanol Production From Microfluidized Wheat Straw by Response Surface Methodology
Turhan, Ozge; Isci, Asli; Mert, Behiç; Sakiyan, Ozge; Donmez, Sedat (2015-11-17)
In this study, wheat straw was pretreated with a microfluidizer to improve its enzymatic hydrolysis and ethanol yields. The pretreatment was performed at various pressures (500, 1000, and 1500bar) and solid loadings (1, 2, and 3%). The microfluidized biomass was then subjected to hydrolysis and simultaneous saccharification and co-fermentation (SSCF) experiments at different enzyme loadings (5, 10, and 15 FPU/g dry wheat straw) using a mutant yeast. The results indicated that the microfluidization method al...
Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (Elsevier BV, 2019-06-01)
Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreat...
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A. Iram, D. Çekmecelioğlu, and A. Demirci, “Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors,” presented at the 2022 ASABE Annual International Meeting, Texas, Amerika Birleşik Devletleri, 2022, Accessed: 00, 2023. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85137569855&origin=inward.