Optical 4D oxygen mapping of microperfused tissue models with tunable in vivo-like 3D oxygen microenvironments

Wesseler, Milan Finn
Johansen, Mathias Norbaek
Kızıltay, Aysel
Mortensen, Kim
Larsen, Niels B.
Sufficient and controllable oxygen supply is essential for in vitro 3D cell and tissue culture at high cell densities, which calls for volumetric in situ oxygen analysis methods to quantitatively assess the oxygen distribution. This paper presents a general approach for accurate and precise non-contact 3D mapping of oxygen tension in high cell-density cultures via embedded commercially available oxygen microsensor beads read out by confocal phosphorescence lifetime microscopy (PLIM). Optimal acquisition conditions and data analysis procedures are established and implemented in a publicly available software package. The versatility of the established method is first demonstrated in model-assisted fluidic design of microperfused 3D printed hydrogel culture chips with the aim of full culture oxygenation, and subsequently for monitoring and maintenance of physiologically relevant spatial and temporal oxygen gradients in the 3D printed chips controlled by static or dynamic flow conditions during 3D culture.


Near-IR absorbing BODIPY derivatives as glutathione-activated photosensitizers for selective photodynamic action.
Turan, IS; Cakmak, FP; Kahraman, Deniz Cansen; Atalay, Rengül; Akkaya, EU (2014-12-01)
Enhanced spatiotemporal selectivity in photonic sensitization of dissolved molecular oxygen is an important target for improving the potential and the practical applications of photodynamic therapy. Considering the high intracellular glutathione concentrations within cancer cells, a series of BODIPY-based sensitizers that can generate cytotoxic singlet oxygen only after glutathione-mediated cleavage of the electron-sink module were designed and synthesized. Cell culture studies not only validate our design,...
Enhancement in Oxygen Reduction Reaction Activity of Nitrogen-Doped Carbon Nanostructures in Acidic Media through Chloride-Ion Exposure
Jain, Deeksha; Mamtani, Kuldeep; Gustin, Vance; Gunduz, Seval; Çelik, Gökhan; Waluyo, Iradwikanari; Hunt, Adrian; Co, Anne C.; Ozkan, Umit S. (2018-07-11)
Nitrogen-doped carbon nanostructures (CNx) are promising cathode materials as catalysts for the oxygen reduction reaction (ORR) in polymer electrolyte membrane (PEM) fuel cells. Incorporation of chlorine into CNx catalysts using a facile methodology can lead to a significant improvement in the ORR activity in acidic media, as confirmed by electrochemical half-cell measurements. The chlorine-containing CNx catalyst (CNx-Cl) is synthesized by soaking CNx powder in 0.3 M HCl. The analysis of near-edge X-ray ab...
Automatic detection of mitochondria from electron microscope tomography images: a curve fitting approach
Tasel, Serdar F.; HASSANPOUR, REZA; Mumcuoğlu, Ünal Erkan; Perkins, Guy; Martone, Maryann (2014-02-18)
Mitochondria are sub-cellular components which are mainly responsible for synthesis of adenosine tri-phosphate (ATP) and involved in the regulation of several cellular activities such as apoptosis. The relation between some common diseases of aging and morphological structure of mitochondria is gaining strength by an increasing number of studies. Electron microscope tomography (EMT) provides high-resolution images of the 3D structure and internal arrangement of mitochondria. Studies that aim to reveal the c...
Optical and structural characterization of mixed halide perovskite thin films by thermal co-evaporation
Yılmaz, Onur; Turan, Raşit; Yerci, Selçuk; Department of Physics (2016)
Cost-effective, easy-to-produce, optically and electrically configurable properties of perovskite allow researchers to perform a profound investigation to better understand its physical and chemical nature. Additionally, band gap tunability by introducing bromide (Br) to replace iodine (I) also makes perovskite a suitable material for tandem solar cells. However, to fully benefit from perovskite cells, hysteresis in current-voltage curve, poor stability and relatively low fill factor in large area devices n...
Optical fluoride sensing with a bay region functionalized perylenediimide dye
Yukruk, Funda (2006-01-01)
A perylenedimiide (PDI) derivative functionalized at the perylene core (bay region) to carry phenyl boronic acid groups was shown to interact with fluoride with changes in the emission and absorption spectrum. These changes are most likely due to fluoride-induced aggregation and/or quenching of the perylenediimide dye. The dye is also selective; among halide ions, fluoride anions generate a significant response. Thus, this class of PDI derivatives is likely to be useful in practical fluoride sensing.
Citation Formats
M. F. Wesseler, M. N. Johansen, A. Kızıltay, K. Mortensen, and N. B. Larsen, “Optical 4D oxygen mapping of microperfused tissue models with tunable in vivo-like 3D oxygen microenvironments,” LAB ON A CHIP, vol. 22, no. 21, pp. 4167–4179, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/100476.