Bactericidal and in vitro osteogenic activity of nano sized cobalt-doped silicate hydroxyapatite

Date

2022-10-01

Author

Alshemary, Ammar Z.
Hussain, Rafaqat
Dalgic, Ali Deniz
Evis, Zafer

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

207
views

0
downloads

Hydroxyapatite (HA) particles with enhanced antibacterial properties can be prepared by integrating metal ions into the crystal structure of the nanoparticles. Cobalt and silicate ions containing HA (Co/Si-HA) with the formula Ca10-xCox(PO4)6-y(SiO4)y(OH)2 (x = 0.2, 0.6, and 1.0 and y = 0.5) was successfully synthesised by using microwave-assisted wet precipitation method. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and inductively coupled plasma mass spectrometry (ICP-MS) techniques were used to characterise the synthesised nanoparticles. The results revealed that the incorporation of SiO44− ions increased the lattice parameters and decreased the crystallite size of HA. However, the incorporation of Co2+ions led to the reduction of lattice parameters and the particle size of the SiHA nanoparticles. In vitro antibacterial activity of materials was evaluated using disk diffusion and minimum inhibitory concentration (MIC) protocols. The findings indicated that incorporating Co2+ ions into SiHA inhibited the growth of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus). The cytotoxicity of materials evaluated using the Sarcoma osteogenic (Saos-2) cell line revealed that they were cytocompatible and exhibited no adverse side effects. The osteogenic differentiation of cells was confirmed by the significant increase in the alkaline phosphatase (ALP) activity by incorporating Co2+/SiO44− ions into the HA crystal structure. Our results show that the nanoparticles prepared in this study have a promising future in biomaterial-tissue engineering applications.

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85132705977&origin=inward
https://hdl.handle.net/11511/101522

Journal

Ceramics International

DOI

https://doi.org/10.1016/j.ceramint.2022.06.128

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Sol-gel synthesis of antibacterial silver-doped silica powders from sodium silicate (water glass) Tufan, Evrim Özdem; Durucan, Caner; Department of Metallurgical and Materials Engineering (2013) Sol-gel processing routes for the synthesis of antibacterial silver-doped silica (Ag-SiO2) powders from an inorganic precursor, sodium silicate (Na2SiO3, water glass), have been established. For the synthesis of SiO2 powders, two different processing approaches were used. In the first one (indirect synthesis), silver ions were incorporated into the immature wet silica gels obtained from sodium silicate by the exposure of the silica gels to aqueous silver nitrate (AgNO3) solutions. In the second one, silver ...
Miscibility of methylmethacrylate-co-methacrylic acid polymer with magnesium, zinc, and manganese sulfonated polystyrene ionomers Alkan, C; Yurtseven, N; Aras, L (2005-01-01) The miscibility of methyl methacrylate-co-methacrylic acid polymer (MMA-MAA) with metal neutralized sulfonated polystyrene ionomers was investigated by viscometry, differential scanning calorimetry (DSC), and Fourier transform infrared radiation spectroscopy (FTIR) techniques. Polystyrene (PS) was sulfonated by acetic anhydride and sulfuric acid and the sulfonation degree was found to be 2.6 mole percent, and 2.6 mole percent sulfonated polystyrene was neutralized by Mg, Zn, and Mn salts. The miscibility be...
Development of highly luminescent and cytocompatible near-IR-emitting aqueous Ag2S quantum dots Hocaoglu, Ibrahim; Cizmeciyan, M. Natali; ERDEM, Rengin; Özen, Can; KURT, Adnan; Sennaroglu, Alphan; Acar, Havva Yagci (2012-01-01) Colloidally stable and highly luminescent near-IR emitting Ag2S quantum dots (NIRQDs) were prepared by a very simple aqueous method using 2-mercaptopropionic acid (2MPA) as a coating. Emission of Ag2S-2MPA NIRQDs can be tuned between 780 and 950 nm. These NIRQDs have photoluminescence quantum yields (PLQY) around 7-39% and exhibit excellent cytocompatibility even at 600 mu g mL(-1) in NIH/3T3 cells. With such improved properties, Ag2S-2MPA NIRQDs have a great potential in practical bio-applications.
Nano calcium phosphates doped with titanium and fluoride ions: sinterability and stability of phases Güngör Geridönmez, Serap; Evis, Zafer; Department of Engineering Sciences (2012) The purpose of this study was to synthesize calcium phosphates doped with titanium and fluoride ions in different combinations. Pure and doped calcium phosphates were synthesized by a precipitation method. The synthesized materials were sintered at 1100ºC and 1300ºC for 1h. The ceramics were characterized by density measurements to determine the effect of sintering temperature. Presence of phases and bonds were characterized by XRD diffraction and FTIR spectroscopy. Grain sizes of the samples were obtained ...
Sorption of As(V) from waters using chitosan and chitosan-immobilized sodium silicate prior to atomic spectrometric determination Boyacı, Ezel; Shahwan, Talal (2010-01-15) A natural biosorbent containing amine functional groups, chitosan, and a novel sorbent, chitosan-immobilized sodium silicate, were prepared and utilized for the selective sorption of As(V) from waters prior to its determination by atomic spectrometric techniques, namely, hydride generation atomic absorption spectrometry (HGAAS) and inductively coupled plasma mass spectrometry (ICP-MS). Chitosan was synthesized from chitin and sodium silicate was used as the immobilization matrix due to its straightforward s...

Citation Formats

A. Z. Alshemary, R. Hussain, A. D. Dalgic, and Z. Evis, “Bactericidal and in vitro osteogenic activity of nano sized cobalt-doped silicate hydroxyapatite,” Ceramics International, vol. 48, no. 19, pp. 28231–28239, 2022, Accessed: 00, 2023. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85132705977&origin=inward.