Ancient Dna Analysis For Correct Identification Of Charred Crop Seeds From Archaeological Excavations At Bronze Age Kaymakçı

Date

2022-10-21

Author

ÖZDEMİR DEĞİRMENCİ, FUNDA
ULUĞ, ASİYE
KANSU, ÇİĞDEM
Luke, Christina
Roosevelt, Chris
Kaya, Zeki

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Archaeobotanical materials subject to aDNA analysis were recovered from the site of Kaymakçı, one of several settlements in the middle Gediz Valley dating to the Middle and Late Bronze Age, c. 3500–4000 years ago. Excavations at Kaymakçı have yielded many charred ancient seeds representing various crop species, including Triticum aestivum/durum L., Hordeum vulgare L., Vicia ervillia (L) Willd., Cicer arietinum L., and Vitis vinifera L., demonstrating crop cultivation practices for both human and animal consumption in the second millennium BCE. This study aimed to provide genetic data to enable more precise identification of charred crop seeds using the ancient DNA (aDNA) approach. aDNA was successfully extracted from representative charred seeds using a modified CTAB method. In general, the low amounts of and degraded quality of DNA from charred plant seeds are the major constrains of aDNA amplifications with PCR. To overcome these problems and obtain high amounts and good quality genomic DNA, the whole genome amplification kit was used. As proof of endogenous DNA, PCR amplification of the 26SrDNA and ribulose-1,5 biphosphate carboxylase (rbcL) gene regions with aDNA was carried out and sequenced. High levels of similarity between the ancient seeds and their modern relatives were observed with the comparative sequence analysis of these gene regions. Paleogenetic analysis of amplifiable aDNA will provide multiple, well-characterized, and potentially highly polymorphic genetic loci to answer archaeobotanical and archaeological questions regarding crop domestication and transport across Anatolia.

Subject Keywords

Ancient DNA, Charred seeds, Kaymakçı, Ribulose-1,5 biphosphate carboxylase gene

URI

https://www.ahievranconference.org/_files/ugd/614b1f_4f7efa6812344bdd878fd263a914fb77.pdf
https://hdl.handle.net/11511/101753

Conference Name

2. Ahi Evran International Conference on Scientific Research, 21-23 Octover 2022

Collections

Department of Biology, Conference / Seminar

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Citation Formats

F. ÖZDEMİR DEĞİRMENCİ, A. ULUĞ, Ç. KANSU, C. Luke, C. Roosevelt, and Z. Kaya, “Ancient Dna Analysis For Correct Identification Of Charred Crop Seeds From Archaeological Excavations At Bronze Age Kaymakçı,” presented at the 2. Ahi Evran International Conference on Scientific Research, 21-23 Octover 2022, Kırşehir, Türkiye, 2022, Accessed: 00, 2023. [Online]. Available: https://www.ahievranconference.org/_files/ugd/614b1f_4f7efa6812344bdd878fd263a914fb77.pdf.