Analysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetida

Date

2012-12-01

Author

Ozgen, Murat
Ozdilek, Asli
BİRSİN, MELEHAT AVCI
Önde, Sertaç
ŞAHİN, DERYA
AÇIKGÖZ, Esvet
Kaya, Zeki

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

234
views

0
downloads

Seven ancient seeds, about 1600 years old, were found during an archaeological excavation in Asar Island which is located in south-western Turkey. These seeds were subjected to germination, in vitro callus induction and molecular characterization experiments to test the viability and plant origin of the seeds. Six of the seven seeds had viable seed components (such as cotyledons) and produced callus tissue in Murashige and Skoog medium supplemented with 2mgl(-1) 6-benzylamino purine (BAP), 0.2 mgl(-1) 1-naphthaleneacetic acid (NAA), 20 gl(-1) sucrose, 2mgl(-1) glycine and 7 gl(-1) agar, but the calli from these seeds failed to yield adventitious shoots. DNA samples from callus tissues produced by ancient seeds in vitro were of good quality. The internal transcribed spacer (ITS) region in nuclear DNA (nDNA) of ancient seeds was amplified successfully. The sequences from amplified ITS DNA products of six ancient seeds indicated that their ITS sequences matched those of Anagyris foetida after subjecting them to BLAST searches in international sequence databases (NCBI). A. foetida is a relict species endemic to the Mediterranean region and used as a herbal medicine. We believe that seed characteristics such as the very hard, extremely smooth and shiny testa, toxic anagyrine alkaloid content and their storage in a pot further improved the longevity of these ancient seeds.

Subject Keywords

Anagyris foetida, Ancient DNA, Ancient seeds, Callus, Internal transcribed spacer, Seed longevity

URI

https://hdl.handle.net/11511/46072

Journal

SEED SCIENCE RESEARCH

DOI

https://doi.org/10.1017/s0960258512000207

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

DNA from ancient cedar wood from King Midas' tomb, Turkey, and Al-Aksa Mosque, Israel Rogers, S; Kaya, Zeki (2006-01-01) Ancient Taurus cedar (Cedrus libani A. Rich) wood samples from the Tumulus of King Midas at the Gordion archaeological site (about 2700 years old), near Ankara, Turkey, and from the Al-Aksa Mosque (about 1500 to 1900 years old), Jerusalem, Israel, were characterized by studying the sequences of ribosomal DNA (rDNA) internal transcribed spacers (ITS1 and ITS2). After extraction of the DNA, the ITS regions were amplified utilizing the polymerase chain reaction, followed by sequencing, BLAST searches for simil...
Characterisation of DNA from archaelogical wheat (Triticum L.) seeds from Anatolia Somel, Mehmet; Kence, Aykut; Akkaya, Mahinur S.; Department of Biotechnology (2003) Ancient DNA analysis of archaeological wheat remains may serve to clarify unknown or controversial points in the history of wheat. In the first part of this study, extraction and amplification of DNA from Anatolian charred ancient wheat seeds obtained from different locations and ages was attempted. None of the our extraction samples yielded any PCR amplification. The possible reasons for this result were investigated by constructing an artificial charring experiment. The results suggest that the chances of...
The ancient DNA and archaeobotanical analysis suggest cultivation of Triticum aestivum subsp. spelta at Yumuktepe and Yenikapi Pottery Neolithic sites in Turkey Degirmenci, Funda O.; ULAŞ, BURHAN; Kansu, Cigdem; Ulug, Asiye; Caneva, Isabella; Asal, Rahmi; Kaya, Zeki (2022-08-01) Archaeobotanical materials subject to aDNA analysis were recovered from Yumuktepe and Yenikapi, two important archaeological sites in Anatolia and date back to the Pottery Neolithic Period i.e., 7th millennium BC. Many charred ancient seeds representing various cereal species including a great number of wheat grains were documented in mentioned sites. Among the cereal seeds, charred wheat samples were tentatively identified as Triticum aestivum subsp. spelta L. or Triticum new glume wheat (NGW) or atypical ...
Assessment of genetic variation of bread wheat varieties using microsatellite markers Akkaya, Mahinur; Buyukunal-Bal, EB (2004-01-01) The DNA fingerprinting profiles of 11 bread wheat cultivars, grown in Turkey, was obtained by using 19 highly polymorphic wheat microsatellites. Up to six randomly selected individuals from each of these cultivars were subjected to analysis. Estimated polymorphism information content (PIC) values among 65 individual genotypes for 19 loci were between 0.36 and 0.87 with an average value of 0.68. The numbers of observed alleles were between 2 and 9, with an average value of 5.42. These relationships were asse...
Archaeological Investigations in the Harbours of Burgaz, Turkey: 2011-2015 field seasons Greene, Elizabeth S.; Leidwanger, Justin; Tuna, Numan (Wiley, 2019-03-01) Between 2011 and 2015 archaeological fieldwork was conducted in the Archaic through Late Antique harbours associated with Burgaz on the Datca Peninsula in south-west Turkey. This work focused on survey and documentation of built features associated with the four harbours, limited stratigraphic excavation, and identification of shipwrecks and seafaring activity outside the harbours. The results offer new insights into the growth, expansion, and eventual abandonment of the port complex, its development alongs...

Citation Formats

M. Ozgen et al., “Analysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetida,” SEED SCIENCE RESEARCH, pp. 279–286, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46072.