Start-up strategies of electromethanogenic reactors for methane production from cattle manure

Ghaderikia, Amin
Taşkın, Bilgin
Yılmazel Tokel, Yasemin Dilşad
This study qualitatively assessed the impacts of different start-up strategies on the performance of methane (CH4) production from cattle manure (CM) in electromethanogenic reactors. Single chamber MECs were operated with an applied voltage of 0.7 V and the impact of electrode acclimatization with a simple substrate, acetate (ACE) vs a complex waste, CM, was compared. Upon biofilm formation on the sole carbon source (ACE or CM), several MECs (ACE_CM and CM_ACE) were subjected to cross-feeding (switching substrate to CM or ACE) during the test period to evaluate the impact of the primary substrate. Even though there was twice as much peak current density via feeding ACE during biofilm formation, this did not translate into higher CH4 production during the test period, when reactors were fed with CM. Higher or similar CH4 production was recorded in CM_CM reactors compared to ACE_CM at various soluble chemical oxygen demand (sCOD) concentrations. Additionally, feeding ACE as primary substrate did not significantly impact either COD removals or coulombic efficiencies. On the other hand, the use of anaerobic digester (AD) seed as an inoculum in CM-fed MECs (CM_CM), relative to no inoculum added MECs (Blank), increased the initial CH4 production rate by 45% and reduced the start-up time by 20%. In CM-fed MECs, Geobacter dominated bacterial communities of bioanodes and hydrogenotrophic methanogen Methanoculleus dominated archaeal communities of biocathodes. Community cluster analysis revealed the significance of primary substrate in shaping electrode biofilm; thus, it should be carefully selected for successful start-up of electromethanogenic reactors treating wastes.
Waste Management


Ghaderikia, Amin; Yılmazel, Yasemin Dilşad; Department of Environmental Engineering (2022-12-22)
Bioelectrochemical methane production, known as electromethanogenesis, provides an emerging technology for carbon recycling via the conversion of carbon dioxide to methane with the additional benefit of simultaneous organic waste reduction. Bioelectrochemical conversion reactions in an electromethanogenic microbial electrolysis cell (MEC) are catalyzed by electro-active biofilm on the electrodes; hence, biofilm formation has a key role in system performance. In this study, the objective was to evaluate the ...
Amendment for increased methane production rate in municipal solid waste landfill gas collection systems
Larson, Steven L.; Martin, William A.; Şengör, Sema Sevinç; Wade, Roy; Altamimi, Faris (2021-06-01)
Optimization of methane production rate can potentially decrease the operational lifetime of the landfill site and assist with better management of methane harvesting from the landfill cells. Increased moisture content in landfill cells is known to increase the rate of methane production. Several natural biopolymers can sustain moisture content in a solid matrix while providing a scaffolding for microbial communities to grow. This research examined the effect of the biopolymer, produced by Rhizobium tropici...
Infrared measurement of biomass combustion in a wire mesh reactor
Uluca, Kıvanç; Kazanç Özerinç, Feyza; Department of Mechanical Engineering (2019)
As a consequence of increasing emissions due to the global energy production, new clean combustion options which utilize biomass are being sought. Prior to using biomass in an industrial boiler, the combustion characteristics of the fuel have to be assessed so that the boiler and furnace can be designed or retrofitted. In this study, olive residue (OR), endogenous agricultural residue of Turkey was investigated using a wire mesh reactor (WMR) coupled with two infrared cameras. These cameras recorded radiome...
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The objective of the study was to identify the impact of co-digesting clarifier skimmings on the overall methane generation from the treatment plant and additional energy value of the increased methane production. Biogas production from co-digesting clarifier skimmings and sewage sludge in pilot-scale fed-batch mesophilic anaerobic digesters has been evaluated. The digester was fed with increasing quantities of clarifier skimmings loads: 1.5, 2.6, 3.5 and 7.0 g COD equivalent/(L.d) (COD: chemical oxygen dem...
Evaluation of novel thermo-resistant Micractinium and Scenedesmus sp for efficient biomass and lipid production under different temperature and nutrient regimes
Sonmez, Cagla; ELÇİN, EVRİM; Akin, Dilan; Öktem, Hüseyin Avni; Yücel, Ayşe Meral (2016-07-01)
Despite the vast interest in microalgae as feedstock for biodiesel production, relatively few studies examined their response to diurnal temperature fluctuation. Here, we describe biomass and lipid productivities and fatty acid profiles of thermo-resistant Micractinium sp. and Scenedesmus sp. grown in batch cultures in a laboratory set-up that mimics a typically warm summer day in Central Anatolia with a 16-h light temperature of 30 degrees C and 8-h dark temperature of 16 degrees C (30 degrees C (day)/16 d...
Citation Formats
A. Ghaderikia, B. Taşkın, and Y. D. Yılmazel Tokel, “Start-up strategies of electromethanogenic reactors for methane production from cattle manure,” Waste Management, vol. 159, pp. 27–38, 2023, Accessed: 00, 2023. [Online]. Available: