Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
DEVELOPMENT OF ELECTROSPUN FIBER COATED THIN FILM MICROEXTRACTION DEVICES FOR RAPID MASS SPECTROMETRIC DETERMINATION OF POLAR CANCER BIOMARKERS
Download
TEZ_MERVE_ÖZTÜRK_TEZ__PDF.pdf
Date
2023-1-25
Author
Öztürk, Merve
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
187
views
44
downloads
Cite This
One of the leading reasons of deaths around the world is cancer, and most deaths are associated with late diagnosis. In this regard, the development of rapid and reliable analytical methods that can be used in routine control and early diagnosis of the cancer is of great importance. Solid phase microextraction (SPME) is a sampling and sample preparation technique which offers a platform for development of tools suitable for clinical applications. Its thin film geometry when is made in sword like shape, so-called coated blade spray (CBS), can be also used as electrospray ionization source in direct to mass spectrometric analysis. However, the current CBS devices have low affinity for polar analytes. In this thesis, new CBS devices providing high extraction kinetics and high affinity for polar analytes were prepared and an analytical method for determination of clinically important polar urinary metabolites was developed. For this purpose, various polymeric nanomaterials were prepared by the electrospinning method. Hydrolyzed polyacrylonitrile coated probe showed the best performance among all tested polymers and was selected to be used in the study. The extraction conditions were optimized for the determination of amino acids (phenylalanine and tryptophan), nucleotides (8-Oxo-2'-deoxyguanosine (8-oxo-dG) and 8-Oxo-2'-deoxyadenosine (8-oxo-dA)) and neurotransmitters (serotonin and 5-hydroxyindoleacetic acid) which are essential biomarkers in cancer diagnosis, in urine. Validation results showed that the limits of quantification of the final method for all the analytes were 50.0 ng/mL except 5-hydroxyindoleacetic acid which was 100.0 ng/mL, and the entire process including sample preparation and analysis takes only 6 minutes.
Subject Keywords
Electrospinning, Coated Blade Spray, Mass Spectrometry, Solid Phase Micro Extraction
URI
https://hdl.handle.net/11511/102544
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Contribution of different types of -omic data on personalized diagnosis of cancer
Tanıl, Ezgi; Kızılilsoley, Nehir; Nikerel, Emrah (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10)
Cancer is a complex disease which is one of the main causes of death globally. Survival rate of cancer increases if early diagnosis and application of appropriate treatment are available [1,2]. Understanding differential response from one patient to another can aid in drug targeting, understanding disease progression and finding efficient treatment strategies. Tumour cells shown to have altered metabolism and the most commonly defined example is the Warburg effect in which tumour cells shown to use lactic a...
Synthesis of novel indole-isoxazole hybrids and evaluation of their cytotoxic activities on hepatocellular carcinoma cell lines
Hawash, Mohammed; Kahraman, Deniz Cansen; Ergun, Sezen Guntekin; Cetin-Atalay, Rengul; BAYTAŞ, SULTAN (2021-12-01)
Background Liver cancer is predicted to be the sixth most diagnosed cancer globally and fourth leading cause of cancer deaths. In this study, a series of indole-3-isoxazole-5-carboxamide derivatives were designed, synthesized, and evaluated for their anticancer activities. The chemical structures of these of final compounds and intermediates were characterized by using IR, HRMS, H-1-NMR and C-13-NMR spectroscopy and element analysis. Results The cytotoxic activity was performed against Huh7, MCF7 and HCT116...
Molecular characterization of the effects of valdecoxib on colon cancer cell lines (ht29 & sw620) using ATR-FTIR spectroscopy
İnan Genç, Aysun; Severcan, Feride; Banerjee, Sreeparna; Department of Biochemistry (2014)
Colorectal cancer is the third most common malignancy and the fourth most frequent cause of cancer deaths worldwide, with 945,000 estimated new cases and 492,000 deaths per year in industrialized countries. Chronic inflammation of the bowel is associated with increased levels of pro-inflammatory cytokines and the increased signaling, which plays important roles in the development of carcinoma. During chronic inflammation COX-2 levels are dramatically increased which is the main enzyme in the arachidonic aci...
PREPARATION OF ELECTROSPUN COATED THIN FILM EXTRACTION DEVICES FOR DETERMINATION OF CLINICALLY IMPORTANT ENDOGENOUS COMPOUNDS IN BIO-FLUIDS
TEMEL, Ezgi Rana; Boyacı, Ezel; Department of Chemistry (2022-12-22)
Changes in plasma concentrations of endogenous compounds such as cholesterol and steroid hormones may cause many vital problems threatening human life. Therefore, the development of novel rapid and reliable analytical methods for the quantitative determination of clinically important compounds in biofluids is of great importance. Coated blade spray (CBS), a technique which is based on solid-phase microextraction (SPME), consists of a sword like device which acts as an extractive tool for sampling and sample...
Cost-effectiveness analysis of a prospective breast cancer screening program in Turkey
Astım, Engin; Voyvoda, Ebru; Yılmaz, H. Hakan; Department of Economics (2011)
Cancer is the second leading cause of death among the world and it has an increasing share among all causes of death. Economical burden of cancer is increasing especially in high and middle-income countries. Leaving cancer in competitive markets would lead to inefficiencies; hence governments should intervene in the market and make public decisions in struggling cancer. Among all cancer types breast cancer has the highest incidence and mortality rates in females. Causes of breast cancer still remains indete...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. Öztürk, “DEVELOPMENT OF ELECTROSPUN FIBER COATED THIN FILM MICROEXTRACTION DEVICES FOR RAPID MASS SPECTROMETRIC DETERMINATION OF POLAR CANCER BIOMARKERS,” M.S. - Master of Science, Middle East Technical University, 2023.