DEVELOPMENT OF ELECTROSPUN FIBER COATED THIN FILM MICROEXTRACTION DEVICES FOR RAPID MASS SPECTROMETRIC DETERMINATION OF POLAR CANCER BIOMARKERS

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Date

2023-1-25

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Öztürk, Merve

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One of the leading reasons of deaths around the world is cancer, and most deaths are associated with late diagnosis. In this regard, the development of rapid and reliable analytical methods that can be used in routine control and early diagnosis of the cancer is of great importance. Solid phase microextraction (SPME) is a sampling and sample preparation technique which offers a platform for development of tools suitable for clinical applications. Its thin film geometry when is made in sword like shape, so-called coated blade spray (CBS), can be also used as electrospray ionization source in direct to mass spectrometric analysis. However, the current CBS devices have low affinity for polar analytes. In this thesis, new CBS devices providing high extraction kinetics and high affinity for polar analytes were prepared and an analytical method for determination of clinically important polar urinary metabolites was developed. For this purpose, various polymeric nanomaterials were prepared by the electrospinning method. Hydrolyzed polyacrylonitrile coated probe showed the best performance among all tested polymers and was selected to be used in the study. The extraction conditions were optimized for the determination of amino acids (phenylalanine and tryptophan), nucleotides (8-Oxo-2'-deoxyguanosine (8-oxo-dG) and 8-Oxo-2'-deoxyadenosine (8-oxo-dA)) and neurotransmitters (serotonin and 5-hydroxyindoleacetic acid) which are essential biomarkers in cancer diagnosis, in urine. Validation results showed that the limits of quantification of the final method for all the analytes were 50.0 ng/mL except 5-hydroxyindoleacetic acid which was 100.0 ng/mL, and the entire process including sample preparation and analysis takes only 6 minutes.

Subject Keywords

Electrospinning, Coated Blade Spray, Mass Spectrometry, Solid Phase Micro Extraction

URI

https://hdl.handle.net/11511/102544

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

M. Öztürk, “DEVELOPMENT OF ELECTROSPUN FIBER COATED THIN FILM MICROEXTRACTION DEVICES FOR RAPID MASS SPECTROMETRIC DETERMINATION OF POLAR CANCER BIOMARKERS,” M.S. - Master of Science, Middle East Technical University, 2023.