PREPARATION OF ELECTROSPUN COATED THIN FILM EXTRACTION DEVICES FOR DETERMINATION OF CLINICALLY IMPORTANT ENDOGENOUS COMPOUNDS IN BIO-FLUIDS

Download

EZGİ RANA TEMEL_YÜKSEK LİSANS TEZİ.pdf

Date

2022-12-22

Author

TEMEL, Ezgi Rana

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

291
views

55
downloads

Changes in plasma concentrations of endogenous compounds such as cholesterol and steroid hormones may cause many vital problems threatening human life. Therefore, the development of novel rapid and reliable analytical methods for the quantitative determination of clinically important compounds in biofluids is of great importance. Coated blade spray (CBS), a technique which is based on solid-phase microextraction (SPME), consists of a sword like device which acts as an extractive tool for sampling and sample preparation, and when combined with mass spectrometry (MS), it functions as an electrospray ionization source. Therefore, it has gained attention in recent years as one of the most promising tools in the analytical toolbox. However, there are not many extractive phases suitable for certain studies. For that reason, this thesis was focused on preparation of new CBS probes with enhanced performance suitable for rapid analysis of spot sized plasma samples. As a first step, poly(divinylbenzene) (PDVB) nanoparticles were synthesized by mini-emulsion polymerization method and then immobilized in polyacrylonitrile (PAN) by electrospinning method. The new PDVB-PAN-coated blades were used to develop a CBS-MS method for determination of cholesterol, progesterone and testosterone in plasma samples. With this method, 10.0 ng/mL limits of quantification (LOQ) could be obtained for testosterone and progesterone with a sample volume of 200.0 µL while for cholesterol the LOQ was 200.0 ng/mL with the sample volume of 30.0 µL. The developed method requires only 7 minutes, including sample preparation and instrumental analysis, showing the applicability of the method for rapid clinical diagnosis.

Subject Keywords

Solid Phase Microextraction, Mass Spectrometry, Coated Blade Spray, Electrospinning, Nanotechnology

URI

https://hdl.handle.net/11511/101974

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

DEVELOPMENT OF ELECTROSPUN FIBER COATED THIN FILM MICROEXTRACTION DEVICES FOR RAPID MASS SPECTROMETRIC DETERMINATION OF POLAR CANCER BIOMARKERS Öztürk, Merve; Boyacı, Ezel; Department of Chemistry (2023-1-25) One of the leading reasons of deaths around the world is cancer, and most deaths are associated with late diagnosis. In this regard, the development of rapid and reliable analytical methods that can be used in routine control and early diagnosis of the cancer is of great importance. Solid phase microextraction (SPME) is a sampling and sample preparation technique which offers a platform for development of tools suitable for clinical applications. Its thin film geometry when is made in sword like shape, so-c...
Investigation Of Nsaıd Lipid İnteractions Membranes Via Spectroscopic And Themoanalytical Techniques. Banerjee, Sreeparna(2011-12-31) Long term regular use of nonsteroidal anti-inflammatory drugs (NSAIDs) has been correlated with reduced risk of variety of cancers. Celecoxib is a new generation NSAID that can selectively inhibit activity of cyclooxygenase-2 (COX-2) enzyme in prostaglandin production pathway The anticarcinogenic effects of Celecoxib are not solely due to the inhibition of COX-2. COX-2 independent effects observed in vitro can be summarized as inhibition of cell cycle progression, induction of apoptosis, and inhibition of ...
Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells ALTAY, AHMET; İrtem Kartal, Deniz; SADİ, GÖKHAN; Güray, Nülüfer Tülün; YAPRAK, AHMET EMRE (2017-01-01) Phase I-II detoxification and antioxidant enzymes are responsible for the detoxification and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in the metabolic activation of xenobiotics. The herb Salicornia freitagii (SF) (Amaranthaceae) is known for its anticancer, antioxidant, antidiabetic and antiinflammatory activities. In this study, we determined the bioactive phenolics in the SF methanol extract and investigat...
Evaluation of methylation profiles of an epidermal growth factor receptor gene in a head and neck squamous cell carcinoma patient group Mutlu, M.; Mutlu, Pelin; Azarkan, S.; Baylr, Ö.; Öcal, B.; Saylam, G.; KORKMAZ, MEHMET HAKAN (2021-03-23) Upregulation of the epidermal growth factor receptor (EGFR) gene has shown an important impact on the development of head and neck cancers due to its important regulation role on multiple cell signaling pathways. The aim of this study was to investigate the methylation pattern of the promoter region of the EGFR gene between head and neck squamous cell carcinoma (HNSCC) patients and a control group. Forty-seven unrelated HNSCC patients, clinically diagnosed at the Department of Otorhinolaryngology, Dlşkapl Y...
PREPARATION AND CHARACTERIZATION OF POLYMER COATED SMALL UNILAMELLAR VESICLES OZDEN, MY; Hasırcı, Vasıf Nejat (1991-09-02) Glucose oxidase was entrapped in small unilamellar vesicles composed of phosphatidylcholine, dicetyl phosphate and cholesterol. Prediction of the enzyme content of liposomes by calculations based on input concentrations of lipid and protein, dimensions of the lipids and the liposomes yielded one protein per vesicle. The entrapment efficiency was experimentally determined to be about 13%. On the other hand the entrapment efficiency for the small chromate ions was found to be significantly lower (0.1%). T...

Citation Formats

E. R. TEMEL, “PREPARATION OF ELECTROSPUN COATED THIN FILM EXTRACTION DEVICES FOR DETERMINATION OF CLINICALLY IMPORTANT ENDOGENOUS COMPOUNDS IN BIO-FLUIDS,” M.S. - Master of Science, Middle East Technical University, 2022.