PREPARATION OF ELECTROSPUN COATED THIN FILM EXTRACTION DEVICES FOR DETERMINATION OF CLINICALLY IMPORTANT ENDOGENOUS COMPOUNDS IN BIO-FLUIDS

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EZGİ RANA TEMEL_YÜKSEK LİSANS TEZİ.pdf

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2022-12-22

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TEMEL, Ezgi Rana

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Changes in plasma concentrations of endogenous compounds such as cholesterol and steroid hormones may cause many vital problems threatening human life. Therefore, the development of novel rapid and reliable analytical methods for the quantitative determination of clinically important compounds in biofluids is of great importance. Coated blade spray (CBS), a technique which is based on solid-phase microextraction (SPME), consists of a sword like device which acts as an extractive tool for sampling and sample preparation, and when combined with mass spectrometry (MS), it functions as an electrospray ionization source. Therefore, it has gained attention in recent years as one of the most promising tools in the analytical toolbox. However, there are not many extractive phases suitable for certain studies. For that reason, this thesis was focused on preparation of new CBS probes with enhanced performance suitable for rapid analysis of spot sized plasma samples. As a first step, poly(divinylbenzene) (PDVB) nanoparticles were synthesized by mini-emulsion polymerization method and then immobilized in polyacrylonitrile (PAN) by electrospinning method. The new PDVB-PAN-coated blades were used to develop a CBS-MS method for determination of cholesterol, progesterone and testosterone in plasma samples. With this method, 10.0 ng/mL limits of quantification (LOQ) could be obtained for testosterone and progesterone with a sample volume of 200.0 µL while for cholesterol the LOQ was 200.0 ng/mL with the sample volume of 30.0 µL. The developed method requires only 7 minutes, including sample preparation and instrumental analysis, showing the applicability of the method for rapid clinical diagnosis.

Subject Keywords

Solid Phase Microextraction, Mass Spectrometry, Coated Blade Spray, Electrospinning, Nanotechnology

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https://hdl.handle.net/11511/101974

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

E. R. TEMEL, “PREPARATION OF ELECTROSPUN COATED THIN FILM EXTRACTION DEVICES FOR DETERMINATION OF CLINICALLY IMPORTANT ENDOGENOUS COMPOUNDS IN BIO-FLUIDS,” M.S. - Master of Science, Middle East Technical University, 2022.