Studies on cAMP-dependent protein kinase in sheep liver.

1984
Javed, Masood-ul-Hassan

Suggestions

Partial purification and characterization of neutral trehalase from commercial baker's yeast, Saccharomyces cerevisiae
Yarar, S; Hamamcı, Haluk; Bakir, U (2000-12-01)
The neutral trehalase of a commercial baker's yeast (S. cerevisiae) strain has been partially purified using ammonium sulfate fractionation and DEAE-cellulose column chromatography techniques. Trehalase was precipitated between 35-50% ammonium sulfate saturation and approximately 5-8 fold purification was achieved. The yeast cAMP-dependent protein kinase was also precipitated in the same fraction and these two proteins were separated by DEAE-cellulose column chromatography. Trehalase became totally inactive...
Investigating physicochemical properties of wetglycated soy proteins
Zia, Muhammed Bin; Öztop, Halil Mecit; Mert, Behiç; Department of Food Engineering (2020-8)
On a lab-scale, glycation is generally a lengthy process which if replicated on an industrial scale, would be costly. In this study, a simplified method for glycating soy protein isolate has been developed. Soy proteins were glycated with allulose, fructose and glucose in the presence of water. The protein-water ratio was changed from ½ to 1 (w/w) and glycation was conducted at 100°C and 130°C for 15 minutes, and samples were also dried at 50°C, 55% relative humidity for 1 day. The physicochemical pro...
Evaluating microemulsions for purification of beta-galactosidase from Kluyveromyces lactis
Mazı, Bekir Gökçen; Hamamcı, Haluk; Department of Food Engineering (2010)
In this study, we evaluated the potential of water-in-oil microemulsions for the separation of beta-galactosidase (lactase) from other proteins. The ability of beta-galactosidase to break down the milk carbohydrate lactose gives the enzyme considerable commercial importance. The extent of solubilization of a commercial Kluyveromyces lactis preparation of beta-galactosidase into microemulsion droplets formed from 200 mM bis (2-ethylhexyl) sodium sulfosuccinate (AOT) in isooctane was measured as a function of...
Prediction of protein subcellular localization based on primary sequence data
Özarar, Mert; Atalay, Mehmet Volkan; Department of Computer Engineering (2003)
Subcellular localization is crucial for determining the functions of proteins. A system called prediction of protein subcellular localization (P2SL) that predicts the subcellular localization of proteins in eukaryotic organisms based on the amino acid content of primary sequences using amino acid order is designed. The approach for prediction is to nd the most frequent motifs for each protein in a given class based on clustering via self organizing maps and then to use these most frequent motifs as features...
Partial purification and CAMP binding properties of bovine cardiac CAMP-dependent protein kinase.
Yücel, Meral; Özer, İnci; Department of Biological Sciences (1980)
Citation Formats
M.-u.-H. Javed, “Studies on cAMP-dependent protein kinase in sheep liver.,” Ph.D. - Doctoral Program, Middle East Technical University, 1984.