Conducting block copolymers of thiophene-capped poly(methyl methacrylate) with pyrrole and their uses in immobilization of enzymes

Alkan, Selmiye
Conducting block copolymers of thiophene capped poly (methyl methacrylate) with pyrrole were synthesized via electrochemical methods. Two types of poly (methyl methacrylate) having thiophene moiety at one end were previously synthesized by atom transfer radical polymerization (ATRP) and they were utilized as the insulating polymer matrices for the synthesis of copolymers. Thiophene ended 4,4'Azobis (4-cyano 3-thiphenemethyl pentanate) (ACTMP) was synthesized from 3-thiophenemethanol and 4,4Azobis (4-cyanopentanoyl chloride). ACTMP was used in the 111synthesis of polystyrene with thiophene terminal groups (TPSt) at both ends. Conducting block copolymers of TPSt and pyrrole were electrochemically prepared using several supporting electrolytes. Electrochemical polymerization in the presence of pyrrole yielded the desired products, however, with thiophene no product was obtained. The obtained copolymers were characterized by spectroscopic and thermal studies. Scanning electron microscopy was used for investigation of surface morphologies. Immobilization of invertase has been studied in three matrices namely pristine polypyrrole and two copolymer matrices. Enzyme immobilization was performed by using sodium dodecyl sulfate (SDS) and p-toluene sulfonic acid and sodium p-toluene sulfonate. In comparison to immobilization of invertase in pure polypyrrole matrix, copolymer matrices were revealed better operational stability. Immobilization of urease in copolymer matrices were performed. Electrolysis media, electrolysis time, enzyme concentration during electrolysis and activity assay conditions were optimized. Urease entrapped in copolymer matrices showed better responses compared to pristine pyrrole in terms of enzyme activity, kinetic parameters and storage stability.


Conducting graft copolymers of pyrrole and thiophene with random copolymers of methyl methacrylate and 3-methylthienyl methacrylate
Guner, Y; Toppare, Levent Kamil; Hepuzer, Y; Yagci, Y (2004-08-01)
Random copolymers of 3-methyl thienylmethacrylate and methyl methacrylate were synthesized via free radical polymerization. Electro-copolymerizations of random copolymers with thiophene and/or pyrrole were carried out in acetonitrile-tetrabutylammonium tetrafluoroborate (TBAFB), water-p-toluene sulfonic acid (PTSA) solvent-electrolyte couples. Oxidative polymerization of thiophene functionalized random copolymer was also achieved by constant current electrolysis and chemical polymerization. The characteriza...
Immobilization of yeast cells in several conducting polymer matrices
Balci, Z; Akbulut, Ural; Toppare, Levent Kamil; Alkan, S; Bakir, U; Yagci, Y (2002-01-01)
Immobilization of yeast cells (Saccharomyces cerevisiae) in different polymer matrices was performed by constant potential electrolysis. These matrices were polypyrrole (PPy); poly(methyl methacrylate)/polypyrrole (,PMMA/PPy) and thiophene-capped poly(methyl methacrylate)/ polypyrrole (TPMMA/PPy). The characterization of PMMA/PPy copolymer was achieved by Fourier-transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The invertase activity of...
Conducting graft copolymers of poly (3-methylthienyl methacrylate) with pyrrole and thiophene
Çırpan, Ali; Toppare, Levent Kamil; YAGCI, Y (2002-12-01)
A thiophene-functionalized methacrylate monomer (3-methylthienyl methacrylate) was synthesized via the esterification of 3-thiophene methanol with methacryloyl chloride. The methacrylate monomer was polymerized by free-radical polymerization in the presence of azobisisobutyronitrile as the initiator. Graft copolymers of poly(3-methylthienyl methacrylate) (PMTM2) and polypyrrole and of PMTM2 and polythiophene were synthesized by constant-potential electrolyses. p-Toluene sulfonic acid, sodium dodecyl sulfate...
Conducting copolymers of thiophene-functionalized polystyrene
Papila, O; Toppare, Levent Kamil; Yagci, Y; Cianga, L (Informa UK Limited, 2004-01-01)
The syntheses of conducting copolymers of thiophene-functionalized polystyrene and pyrrole (PS/PPy) were achieved using p-toluene sulfonic acid (PTSA) as the supporting electrolyte via constant potential electrolysis technique. Characterization of the samples was performed by a combination of techniques: cyclic voliammetry (CV), thermogravimetric analysis (TGA), differential scanning colorimetry (DSC), scanning electron microscopy (SEM), NMR, and FT-IR analyses. The conductivities were measured by the four-...
Electrically conductive polymer grafts prepared by electrochemical polymerization of pyrrole onto poly[(methyl methacrylate)-co-(2-(N-pyrrolyl) ethyl methacrylate)] electrodes
Balci, N; Akbulut, Ural; Toppare, Levent Kamil; Stanke, D; Hallensleben, ML (1998-01-01)
Pyrrole was grafted on poly[(methyl methacrylate)-co-(2-(N-pyrrolyl) ethyl methacrylate)] (PMMA-co-PEMA) using constant potential electrolyses. The thermal stability of PMMA-co-PEMA was improved as a result of electrochemical grafting with pyrrole. The electrochemical behavior of the films was studied by cyclic voltammetry. Pyrrole was found to be electroactive on PMMA-co-PEMA electrodes.
Citation Formats
S. Alkan, “Conducting block copolymers of thiophene-capped poly(methyl methacrylate) with pyrrole and their uses in immobilization of enzymes,” Ph.D. - Doctoral Program, Middle East Technical University, 2002.