Conducting block copolymers of thiophene-capped poly(methyl methacrylate) with pyrrole and their uses in immobilization of enzymes

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2002
Alkan, Selmiye
Conducting block copolymers of thiophene capped poly (methyl methacrylate) with pyrrole were synthesized via electrochemical methods. Two types of poly (methyl methacrylate) having thiophene moiety at one end were previously synthesized by atom transfer radical polymerization (ATRP) and they were utilized as the insulating polymer matrices for the synthesis of copolymers. Thiophene ended 4,4'Azobis (4-cyano 3-thiphenemethyl pentanate) (ACTMP) was synthesized from 3-thiophenemethanol and 4,4Azobis (4-cyanopentanoyl chloride). ACTMP was used in the 111synthesis of polystyrene with thiophene terminal groups (TPSt) at both ends. Conducting block copolymers of TPSt and pyrrole were electrochemically prepared using several supporting electrolytes. Electrochemical polymerization in the presence of pyrrole yielded the desired products, however, with thiophene no product was obtained. The obtained copolymers were characterized by spectroscopic and thermal studies. Scanning electron microscopy was used for investigation of surface morphologies. Immobilization of invertase has been studied in three matrices namely pristine polypyrrole and two copolymer matrices. Enzyme immobilization was performed by using sodium dodecyl sulfate (SDS) and p-toluene sulfonic acid and sodium p-toluene sulfonate. In comparison to immobilization of invertase in pure polypyrrole matrix, copolymer matrices were revealed better operational stability. Immobilization of urease in copolymer matrices were performed. Electrolysis media, electrolysis time, enzyme concentration during electrolysis and activity assay conditions were optimized. Urease entrapped in copolymer matrices showed better responses compared to pristine pyrrole in terms of enzyme activity, kinetic parameters and storage stability.

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Citation Formats
S. Alkan, “Conducting block copolymers of thiophene-capped poly(methyl methacrylate) with pyrrole and their uses in immobilization of enzymes,” Ph.D. - Doctoral Program, Middle East Technical University, 2002.