Immobilization of yeast cells in several conducting polymer matrices

Balci, Z
Akbulut, Ural
Toppare, Levent Kamil
Alkan, S
Bakir, U
Yagci, Y
Immobilization of yeast cells (Saccharomyces cerevisiae) in different polymer matrices was performed by constant potential electrolysis. These matrices were polypyrrole (PPy); poly(methyl methacrylate)/polypyrrole (,PMMA/PPy) and thiophene-capped poly(methyl methacrylate)/ polypyrrole (TPMMA/PPy). The characterization of PMMA/PPy copolymer was achieved by Fourier-transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The invertase activity of immobilized yeast cells was determined. Optimum temperature, Michaelis-Menten constants and maximum reaction rates of the enzyme electrodes were compared with those of free yeast cells. The operational and storage stabilities of three different immobilization systems were analyzed.


Immobilization of invertase in conducting polymer matrices
Selampinar, F; Akbulut, Ural; Ozden, MY; Toppare, Levent Kamil (1997-09-01)
This paper reports a novel approach in the electrode immobilization of an enzyme, invertase, by electrochemical polymerization of pyrrole in the presence of enzyme. The polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes were constructed by the entrapment of enzyme in conducting matrices during electrochemical polymerization of pyrrole. This study involves the preparation and characterization of polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes under conditions compatible...
Immobilization of invertase in functionalized copolymer matrices
Erginer, Reyhan; Toppare, Levent Kamil; Alkan, Selmiye; Bakir, Ufuk (Elsevier BV, 2000-10)
In this study, immobilization of invertase on functionalized polymer electrodes constructed with pyrrole-capped polyazotetrahydrofuran and polytetrahydrofuran-block-polystyrene copolymer matrices was performed. Immobilization in these enzyme electrodes was carried out by file entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole. Sodium dodecyl sulphate was used as the supporting electrolyte in the preparation of enzyme electrodes. The effects of temperatur...
Immobilization of invertase in conducting polypyrrole polytetrahydrofuran graft polymer matrices
Kizilyar, N; Akbulut, Ural; Toppare, Levent Kamil; Ozden, MY; Yagci, Y (1999-06-24)
Four different polypyrrole/polytetrahydrofuran/invertase electrodes were constructed by the entrapment of invertase in conducting polymer matrices via electropolymerization. Immobilization of the enzyme was achieved by the application of a 1.1 V constant potential to a platinum electrode for 30 min in a solution containing 0.01 M pyrrole, 0.4 mg/ml invertase and 0.4 mg/ml sodium dodecyl sulphate. Performance of each electrode was optimized by examining the effects of pH and temperature on the properties of ...
Immobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogels
Arica, MY; Öktem, Hüseyin Avni; Oktem, Z; Tuncel, SA (1999-09-01)
Catalase was entrapped in thermally reversible poly (isopropylacrylamide-co-hydroxyethyl-methacrylate) (pNIPAM/HEMA) copolymer hydrogels. The thermoresponsive hydrogels, in cylindIl-rical geometry, were prepared in an aqueous buffer by redox polymerization. It was observed that upon entrapment, the activity retention of catalase was decreased between 47 and 14%, and that increasing the catalase loading of hydrogel adversely affected the activity. The kinetic behaviour of the entrapped enzyme was investigate...
Conducting block copolymers of thiophene-capped poly(methyl methacrylate) with pyrrole and their uses in immobilization of enzymes
Alkan, Selmiye; Toppare, Levent Kamil; Bakır, Ufuk; Department of Chemistry (2002)
Conducting block copolymers of thiophene capped poly (methyl methacrylate) with pyrrole were synthesized via electrochemical methods. Two types of poly (methyl methacrylate) having thiophene moiety at one end were previously synthesized by atom transfer radical polymerization (ATRP) and they were utilized as the insulating polymer matrices for the synthesis of copolymers. Thiophene ended 4,4'Azobis (4-cyano 3-thiphenemethyl pentanate) (ACTMP) was synthesized from 3-thiophenemethanol and 4,4Azobis (4-cyanope...
Citation Formats
Z. Balci, U. Akbulut, L. K. Toppare, S. Alkan, U. Bakir, and Y. Yagci, “Immobilization of yeast cells in several conducting polymer matrices,” JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, pp. 183–197, 2002, Accessed: 00, 2020. [Online]. Available: