Immobilization of yeast cells in several conducting polymer matrices

Date

2002-01-01

Author

Balci, Z
Akbulut, Ural
Toppare, Levent Kamil
Alkan, S
Bakir, U
Yagci, Y

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Immobilization of yeast cells (Saccharomyces cerevisiae) in different polymer matrices was performed by constant potential electrolysis. These matrices were polypyrrole (PPy); poly(methyl methacrylate)/polypyrrole (,PMMA/PPy) and thiophene-capped poly(methyl methacrylate)/ polypyrrole (TPMMA/PPy). The characterization of PMMA/PPy copolymer was achieved by Fourier-transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The invertase activity of immobilized yeast cells was determined. Optimum temperature, Michaelis-Menten constants and maximum reaction rates of the enzyme electrodes were compared with those of free yeast cells. The operational and storage stabilities of three different immobilization systems were analyzed.

Subject Keywords

Immobilization, Yeast, Invertase, Polypyrrole, Poly(methyl methacrylate)

URI

https://hdl.handle.net/11511/45943

Journal

JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY

DOI

https://doi.org/10.1081/ma-120013259

Collections

Department of Chemistry, Article

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Citation Formats

Z. Balci, U. Akbulut, L. K. Toppare, S. Alkan, U. Bakir, and Y. Yagci, “Immobilization of yeast cells in several conducting polymer matrices,” JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, pp. 183–197, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/45943.