Identification of the genes involved in phytosiderophore" synthesis and metal ion uptake in wheat using RT-PCR

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2003

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Aktaş, Yasemin

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Soils in many agricultural areas have high pH, resulting in low availability of zinc and iron. Plants grown on such soils suffer from either Zn or Fe deficiency or both. The efficient plant genotypes grown normally in calcerous soils were found to evolve some strategies to acquire the iron which is in insoluble form. Iron efficient graminaceous monocots release iron chelating substances, mugineic acid family phytosiderophores (MAs), in response to iron deficiency stress. Several researchers have suggested that phytosiderophores also can play role in grass Zn nutrition and thus it may be possible that it is the uptake mechanism for Zn efficiency. Several possible genes that take role in phytosiderophore synthesis or found to be induced under iron deficient conditions were identified in several organisms but not on wheat. In this study, the efficient barley cultivar Tokak-157, efficient wheat cultivar Kıraç-66 and relatively less efficient wheat cultivar BDMM-19 were grown in normal growth conditions for 1 week and transfered to zinc deficient, iron deficient and both zinc and iron deficient nutrient solutions. After growing 1 week on these conditions, plants grown on both zinc and iron deficient nutrient solutions were retransfered to zinc and iron sufficient conditions. Degenerate primers were designed for the conserved regions of previously identified genes that take role in phytosiderophore synthesis or induced under iron deficient conditions and RT-PCRs were performed. The complete open reading frame of IDI-1(Iron deficiency induced-1) gene and the putative gene fragment for SAM-s (S-adenosylmethionine synthetase) were identified.

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http://etd.lib.metu.edu.tr/upload/3/1252857/index.pdf
https://hdl.handle.net/11511/13446

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

Y. Aktaş, “Identification of the genes involved in phytosiderophore” synthesis and metal ion uptake in wheat using RT-PCR,” M.S. - Master of Science, Middle East Technical University, 2003.