Fatty acid methyl ester analysis of bacterşal isolates from salt lake, Turkey and characterization of their extracellular enzymes

Download

index.pdf

Date

2004

Author

Bahçeci, Hümeyra

Metadata

Show full item record

Item Usage Stats

262
views

124
downloads

In this study, 11 bacterial isolates from Salt Lake,Turkey were identified by using fatty acid methyl ester (FAME) analysis. They were screened for production of industrially important enzymes xylanase, cellulase, a-amylase and protease. These enzymes were characterized in terms of enzyme activity, stability, optimum temperature and optimum pH. One of the isolates was identified as Bacillus pumilus, and two of them were identified as Bacillus subtilis. Other isolates were determined to be Bacillus licheniformis. All the isolates were determined to produce xylanase. Optimum temperatures and optimum pH values of xylanases were 50-55 °C and pH 7.0-8.0. Xylanases were quite stable up to pH 8.0 and 70 °C. Isolates were not significant cellulase producers. Four of the isolates did not produce any cellulase enzyme and the rest produced negligible amounts of cellulase. Therefore, xylanases from the isolates were promising for pulp and paper industry, which requires cellulase free and stable xylanases. All the isolates produced appreciable quantities of a-amylase. Optimum temperatures and optimum pH values of a-amylases 60-80 °C and pH 7.0-8.0. a-Amylases were quite stable up to pH 9.0 and 80 °C. a-Amylases from the isolates were promising for starch processing industry, which requires a-amylases stable at high temperatures and for detergent industry, which requires a-amylases stable at alkaline pH values. Considerable protease productions were achieved by all the isolates. TTG 2 was the best protease producer with 271 U/ml. Optimum temperatures and optimum pH values of proteases were 50-60 °C and pH 7.0-7.4. Proteases were quite stable up to pH 9.0 and 80 °C. Proteases from the isolates were promising for detergent and leather industry, in which proteases must be stable at alkaline pH values.

Subject Keywords

Antigens.

URI

http://etd.lib.metu.edu.tr/upload/3/12605483/index.pdf
https://hdl.handle.net/11511/14533

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Trace level determination of beryllium in natural and flavored mineral waters after pre-concentration using activated carbon KILINÇ, ERSİN; Bakirdere, Sezgin; YAMAN, MEHMET (Informa UK Limited, 2011-01-01) The concentrations of beryllium (Be) in natural and flavored mineral water samples were determined by flame atomic absorption spectrophotometer (FAAS) after pre-concentration based on the complexation of Be(+2) with a mixture of acetylacetone (pentane-2,4-dione) plus morin (3,5,7,2',4'-pentaoxyflavone) and adsorption on activated carbon. The adsorbed complex was eluted with 1.5 ml of 2.0 M HNO(3) and evaporated to dryness. After adding 1.5 ml of 2 M HNO(3) and centrifuging, Be in acid solution was determine...
Kinetic analysis of aspergillus oryzae cultivations on starch Bayındırlı, Alev; UNGAN, S (Informa UK Limited, 1991-01-01) Cultivation of Aspergillus oryzae on starch is described as a combination of two rate processes: Starch hydrolysis and the cellular activities of the fungi including growth, enzyme production and maintenance. Kinetic models are presented to describe growth, enzyme production, starch hydrolysis and uptake of the hydrolysis products. Numerical values of the model parameters indicated that the rate controlling step of A. oryzae growth on starch was not starch hydrolysis, but the substrate uptake process. Gluco...
SODIUM, RUBIDIUM AND CESIUM UPTAKE ON LOS-TRANCOS CLAY GOKTURK, H; DYER, A (Springer Science and Business Media LLC, 1994-10-03) The uptake of sodium, caesium and rubidium onto Los Trances clay was studied using the batch method. Sodium-22, rubidium-86 and caesium-137 were used as tracers. In most of the experiments cation concentrations used were 0.01 N. Triple distilled and deionized water ware used and the grain size of all solid particles was less than or equal to 53 mu m. In all cases a few hours of shaking was enough to reach the equilibrium. The ion exchange isotherm experiments were performed by using ammonium forms of the cl...
Sorption of radioactive cesium and barium ions onto solid humic acid Celebi, O.; Kilikli, A.; ERTEN, HASAN NİYAZİ (Elsevier BV, 2009-09-15) In this study, the sorption behavior of two important fission product radionuclides ((137)Cs and (140)Ba) onto sodium form of insolubilized humic acid (INaA) were investigated as a function of time, cation concentration and temperature, utilizing the radiotracer method. The sorption processes are well described by both Freundlich and Dubinin-Radushkevich type isotherms. Thermodynamic constants such as: free energy (Delta G(ads)), enthalpy (Delta H(ads)), entropy (Delta S(ads)) of adsorption were determined....
Anaerobic acidification of sugar-beet processing wastes: Effect of operational parameters Alkaya, Emrah; Demirer, Göksel Niyazi (Elsevier BV, 2011-01-01) The objective of this study was to maximize the hydrolysis and acidification of sugar-beet processing wastewater and beet pulp for volatile fatty acid (VFA) production through acidogenic anaerobic metabolism. Experiments were conducted to determine the optimum operational conditions (HRT, waste-mixing ratio and pH) for effective acidification in daily-fed, continuously mixed anaerobic reactors. For this purpose, reactors were operated at 35 +/- 1 degrees C with different combinations of HRT (2-4 days), wast...

Citation Formats

H. Bahçeci, “Fatty acid methyl ester analysis of bacterşal isolates from salt lake, Turkey and characterization of their extracellular enzymes,” M.S. - Master of Science, Middle East Technical University, 2004.