Transformation of tobacco (Nicotiana tabaccum) with antimicrobial pflp gene and analysis of transgenic plants

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2006

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Tuncer, Taner

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The objective of this study was to transform sweet pepper ferredoxin-like protein (PFLP) gene, which has antimicrobial properties, to tobacco and investigate the disease resistance abilities of transgenic tobacco. This protein interacts with another protein, harpin that is produced by the bacteria which is invading the plant tissues, and stimulates hypersensitivity response in plants, thus the spreading of disease is limited. Gene transfer was achieved to tobacco by Agrobacterium- mediated method and with indirect organogenesis; the explants were grown on selective media and then transferred to jars and pots respectively. Molecular and genetic analyses such as PCR, RT-PCR, Sequence Analysis and Northern Blot, were performed with plants which their seeds survived and grew on selective medium and also gave positive reactions for GUS histochemical assay. Finally, with putative transgenic plants, some hypersensitive response assays were carried out with Pseudomonas syringae and it was observed that the recovered plants showed hypersensitive response (HR) in the preliminary tests. These results indicated that putative transgenic tobacco plants which carry pflp transgene, can be used in disease resistance studies.

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Plant Protection.

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http://etd.lib.metu.edu.tr/upload/12607007/index.pdf
https://hdl.handle.net/11511/15515

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

T. Tuncer, “Transformation of tobacco (Nicotiana tabaccum) with antimicrobial pflp gene and analysis of transgenic plants,” M.S. - Master of Science, Middle East Technical University, 2006.