First reference map for Phanerochaete chrysosporium proteome

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2006

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Yıldırım, Volkan

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In this study, the soluble protein fraction of P. chrysosporium grown under standard conditions was analyzed by using 2D-PAGE approach and a 2-D reference map was constructed. 910 spots could be separated and detected on Coomassie-stained 2-D gels by the help of Delta2D image analysis software. 720 spots could be cut from the master gel and were subjected to MALDI-TOF MS analysis followed by MASCOT search. A total of 517 spots out of 720 were assigned to specific accession numbers from the P. chrysosporium genome database. Further analysis of the data revealed 314 different gene products (distinct ORFs). The theoretical pI and MW values were plotted against the experimental migration distances. Results indicated the existence of 124 protein spots whose horizontal migration differed significantly from the expected migration according to the calculated pI values and 52 spots with an apparent molecular weight that is significantly different from their theoretical molecular weight. While protein modification could be predicted by these analyses, the main support was the presence of multiple spots of the same gene product. As much as 118 ORFs yielded multiple spots on the master gel, corresponding to 37.5% of the all distinct ORFs identified in this work. The relative abundance of each of the 517 identified polypeptides was calculated in terms of spot intensity. The majority of the most abundant proteins were found to be housekeeping ones. When the relative distribution of the proteins into four main functional categories was taken into consideration, أMetabolismؤ appeared the most important category with a share of 50.6% among identified proteins. However, among the functional classes, أPosttranslational modifications, protein turnover, chaperonesؤ which is listed under the main category أCellular Processing and Signallingؤ was represented by the highest number (104) of

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Microbiology.

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http://etd.lib.metu.edu.tr/upload/12607062/index.pdf
https://hdl.handle.net/11511/15720

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

V. Yıldırım, “First reference map for Phanerochaete chrysosporium proteome,” M.S. - Master of Science, Middle East Technical University, 2006.