Growth of agriculturally important Pseudomonas spp. and Azotobacter Chroococcum on beer waste and observation of their survival in peat

Abat, Benek
In this study agriculturally important Pseudomonas spp. which may solubilize phosphate and Azotobacter chroococcum which can fix atmospheric nitrogen were grown on waste beer with 4 different concentrations and conditions for best growth were determined. Having potential of use as biofertilizers, they were put in the carrier material peat and survivals of them were observed for 3 months at three different temperatures. Biofertilizer can be defined as a substance which contains living microorganisms which, when applied to seed, plant surface, or soil, colonizes the rhizosphere or the interior of the plant and promotes growth by replacing soil nutrients or making nutrients more available or increasing plant access to nutrients. In order to benefit from the biofertilizers, viable and active microorganisms in high numbers must be present which requires high quality inoculants. The carrier substrate is a critical part of the product formulation and must be capable of supporting high numbers of the intended microbe(s). It was found that Pseudomonas spp. can solubilize phosphate. Furthermore, conditions for best growth for both bacteria were determined as 30 % of waste beer. Peat was found as an appropriate carrier due to preservation of viable cells for 3 months at 0 ºC, 20 ºC and 30 ºC. However, peat couldn’t support high numbers of Pseudomonas spp. at 30 ºC.


Determination of metabolic bottlenecks using reaction engineering principles in serine alkaline protease production by recombinant bacillus sp.
Telli, İlkin Ece; Çalık, Pınar; Department of Chemical Engineering (2004)
In this study, firstly, bioprocess characteristics for Serine Alkaline Protease (SAP) production, using recombinant Bacillus subtilis carrying pHV1431::subC, were examined. The cell concentration, substrate concentration, SAP activity and SAP synthesis rate profiles demonstrated that the system reaches to a steady state in terms of cell growth and SAP synthesis between t=15-25 h, therefore, this time interval is appropriate to employ both metabolic flux analysis and metabolic control analysis, which apply s...
Development of PCR methods for detection and quantification of genetically modified maize
Jabbari Farhoud, Houman; Gültekin, Güzin Candan; Department of Biotechnology (2010)
This study describes development of methods for screening, identification and quantification of genetic modifications in maize samples. Totally 88 maize samples were collected randomly throughout Turkey in three years from 2006 to 2008 and were analyzed. Two maize samples that were detected as GM positive in previous studies were selected as positive controls. Following the DNA extraction by manual CTAB method, conventional PCR methods were employed for screening of genetic modifications in samples by detec...
Recombinant therapeutic protease production by Bacillus sp.
Korkmaz, Nuriye; Çalık, Pınar; Department of Chemical Engineering (2007)
The first aim of this study is the development of extracellular recombinant therapeutic protease streptokinase producing Bacillus sp., and the second aim is to determine fermentation characteristics for streptokinase production. In this context, the signal (pre-) DNA sequence of B.licheniformis (DSM1969) extracellular serine alkaline protease enzyme gene (subC: Acc. No. X03341) was ligated to 5’ end of the streptokinase gene (skc: Acc. No. S46536) by SOE (Gene Splicing by Overlap Extension) method through P...
Effects of pH and feeding strategy on metabolite profiling of beta-lactamase producing bacillus licheniformis
İler, Nazar; Çalık, Pınar; Department of Chemical Engineering (2005)
In this study, the effects of pH and different feeding modes on beta-lactamase production and cell metabolism were investigated with Bacillus licheniformis (ATCC 25972). For this purpose, first, the effects of pH on beta-lactamase activity, cell formation, substrate consumption, as well as intracellular sodium, potassium, ammonium ion, amino acid and organic acid concentrations were investigated in V= 3.0 dm3 batch bioreactors consisting of temperature, pH, foam, stirring rate and dissolved oxygen controls....
Effects of antimitotic agents on haploid plant production from unpollinated ovules of sugar beet (beta vulgaris l.)
Gürel, S.; Gürel, E.; Kaya, Zeki; Erdal, M.; Güler, E. (Informa UK Limited, 2003-01-01)
The effects of antimicrotubule agents on haploid embryo formation from unpollinated ovules of sugar beet (Beta vulgaris L.) were investigated. The antimitotic agent colchicine (at 100 and 150 mg/l) and trifluralin (at 5.0 mg/l) increased the frequency of haploid embryo formation whereas pronamide (at 76.9 and 128.2 mg/l) and trifluralin (at 3.4 mg/l) decreased. Ovules that were non-treated with antimicrotuble agents (i.e., ovules of the control treatment) produced higher percentages of haploid embryos (4.25...
Citation Formats
B. Abat, “Growth of agriculturally important Pseudomonas spp. and Azotobacter Chroococcum on beer waste and observation of their survival in peat,” M.S. - Master of Science, Middle East Technical University, 2006.