Immobiliation of glucose oxidase and polyphenol oxidase in conducting copolymer of pyrrole functionalized polystyrene with pyrrole

Ekinci, Olçun
Electrochemical polymerization of pyrrole functionalized polystyrene (PStPy) with pyrrole was carried out in water-sodium dodecyl sulfate solvent-electrolyte couple. Characterization of the resulting copolymer was performed via Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and four probe conductivity measurements. Glucose oxidase and polyphenol oxidase enzymes were immobilized in polypyrrole (PPy) and conducting copolymer of pyrrole functionalized polystyrene with pyrrole (P(PStPy-co-Py). Resulting enzyme electrodes were characterized by kinetic parameters; Vmax and Km. Behavior of enzyme electrodes upon temperature and pH changes were investigated. Glucose oxidase electrode was used for the determination of glucose in orange juice and polyphenol oxidase electrode was used for the determination of polyphenolic compounds in red wine.


Covalent immobilization of glucose isomerase on poly(2-hydoxyethyl methacrylate) particles
Yıldız, Ümit Hakan; Hasırcı, Nesrin; Department of Chemistry (2004)
In this study, poly (2-hydroxyethyl methacrylate), P(HEMA), particles were prepared by suspension polymerization of the monomer 2-hydroxyethyl methacrylate with addition of ethylene glycol dimethyacrylate, EGDMA, as cross linker. Glucose isomerase, GI, enzyme was covalently immobilized on the prepared P(HEMA) particles after activation of the particles with cyanuric chloride. The activities of the free and immobilized enzymes were measured with Ethanol-Carbazole method. The immobilization of GI on P(HEMA) p...
Synthesis, characterization and electrical properties of diazophenylene and diazodiphenylene bridged co, ni, cu, ce, and er phthalocyanine polymers
Alkan, Cemil; Alkan, Cemil; Department of Polymer Science and Technology (2004)
In this research, diazophenylene and diazodiphenylene bridged metal-phthalocyanine polymers were produced from diazonium salt of diaminophenylene/bensidin and pre-synthesized tetraamino metal phthalocyanines. Tetraamino metal phthalocyanine complexes of Co, Ni, Cu, Ce, and Er were obtained by reducing tetranitro metal phthalocyanine complexes synthesized from 3-nitrophthalic anhydride, urea, metal salt, and ammonium molybdate catalyst. Complexes and polymers were characterized by Fourier Transform Infrared ...
Biosensor based on interpenetrated polymer network of alginic acid and poly(1-vinylimidazole )
Kartal, Müjgan; Toppare, Levent Kamil; Department of Chemistry (2008)
A new proton conductor polymer was prepared using alginic acid (AA) and poly (1-vinylimidazole) (PVI). The polymer network was obtained by mixing AA and PVI at various stoichiometric ratios, x (molar ratio of the monomer repeat units). The AA/PVI network was characterized by elemental analysis (EA) and FT-IR spectroscopy. Potential use of this network in enzyme immobilization was studied. Enzyme entrapped polymer networks (EEPN) were produced by immobilizing invertase and tyrosinase (PPO) in the AA/PVI netw...
Syntheses of benzotriazole bearing donor acceptor type random copolymers for full visible light absorption
Öktem, Gözde; Toppare, Levent Kamil; Department of Chemistry (2011)
The synthesis and preliminary optoelectronic properties of a series of donor-acceptor (DA) type polymers differing by the acceptor units in the polymer backbone were investigated. Polymers CoP1, CoP2 and CoP3 were designed to yield alternating DA segments with randomly distributed different acceptor units along polymer backbone. The combination of neutral state red colored and neutral state green colored materials resulted in different neutral state colors with respect to their additional acceptor unit. 5,8...
Conductive and electrochromic properties of poly(p-phenylene vinylene)
Öztürk, Tuğba; Küçükyavuz, Zuhal; Department of Chemistry (2005)
P-xylene-bis(diethylsulphonium chloride) (PXBDC) monomer was synthesized by the reaction of a,a-dichloro-p-xylene with tetrahydrothiophene or diethyl sulphide. Electrochemical behavior of this monomer (PXBDC) was examined by cyclic voltametry. Polymerization was achieved both by using electrochemical and chemical polymerization techniques. In the electrochemical technique, PPV was synthesized by constant potential electrolysis in acetonitrile-tetrabutylammonium tetrafluoroborate (TBAFB) solvent-electrolyte ...
Citation Formats
O. Ekinci, “Immobiliation of glucose oxidase and polyphenol oxidase in conducting copolymer of pyrrole functionalized polystyrene with pyrrole,” M.S. - Master of Science, Middle East Technical University, 2006.