Immobiliation of glucose oxidase and polyphenol oxidase in conducting copolymer of pyrrole functionalized polystyrene with pyrrole

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2006
Ekinci, Olçun
Electrochemical polymerization of pyrrole functionalized polystyrene (PStPy) with pyrrole was carried out in water-sodium dodecyl sulfate solvent-electrolyte couple. Characterization of the resulting copolymer was performed via Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and four probe conductivity measurements. Glucose oxidase and polyphenol oxidase enzymes were immobilized in polypyrrole (PPy) and conducting copolymer of pyrrole functionalized polystyrene with pyrrole (P(PStPy-co-Py). Resulting enzyme electrodes were characterized by kinetic parameters; Vmax and Km. Behavior of enzyme electrodes upon temperature and pH changes were investigated. Glucose oxidase electrode was used for the determination of glucose in orange juice and polyphenol oxidase electrode was used for the determination of polyphenolic compounds in red wine.

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Citation Formats
O. Ekinci, “Immobiliation of glucose oxidase and polyphenol oxidase in conducting copolymer of pyrrole functionalized polystyrene with pyrrole,” M.S. - Master of Science, Middle East Technical University, 2006.