Biosensor based on interpenetrated polymer network of alginic acid and poly(1-vinylimidazole )

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2008
Kartal, Müjgan
A new proton conductor polymer was prepared using alginic acid (AA) and poly (1-vinylimidazole) (PVI). The polymer network was obtained by mixing AA and PVI at various stoichiometric ratios, x (molar ratio of the monomer repeat units). The AA/PVI network was characterized by elemental analysis (EA) and FT-IR spectroscopy. Potential use of this network in enzyme immobilization was studied. Enzyme entrapped polymer networks (EEPN) were produced by immobilizing invertase and tyrosinase (PPO) in the AA/PVI network. Additionally, the maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) were investigated for the immobilized invertase and enzymes. Also, temperature and pH optimization, operational stability and shelf life of the polymer network were examined.
Citation Formats
M. Kartal, “Biosensor based on interpenetrated polymer network of alginic acid and poly(1-vinylimidazole ),” M.S. - Master of Science, Middle East Technical University, 2008.